Repozitorij Univerze v Novi Gorici

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Naslov:The effect of serum withdrawal on the protein profile of quiescent human dermal fibroblasts in primary cell culture.
Avtorji:ID Federica, Boraldi, University of Modena and Reggio Emilia (Avtor)
ID Giulia, Annovi, University of Modena and Reggio Emilia (Avtor)
ID Chiara, Paolinelli Devincenzi, University of Modena and Reggio Emilia (Avtor)
ID Roberta, Tiozzo, University of Modena and Reggio Emilia (Avtor)
ID Daniela, Quaglino, University of Modena and Reggio Emilia (Avtor)
Datoteke: Gradivo nima datotek, ki so prostodostopne za javnost. Gradivo je morda fizično dosegljivo v knjižnici fakultete, zalogo lahko preverite v COBISS-u. Povezava se odpre v novem oknu
Jezik:Angleški jezik
Vrsta gradiva:Delo ni kategorizirano
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:UNG - Univerza v Novi Gorici
Opis:The effect of serum deprivation on proliferating cells is well known, in contrast its role on primary cell cultures, at confluence, has not been deeply investigated. Therefore, in order to explore the response of quiescent cells to serum deprivation, ubiquitous mesenchymal cells, as normal human dermal fibroblasts, were grown, for 48 h after confluence, in the presence or absence of 10% FBS. Fibroblast behaviour (i.e. cell morphology, cell viability, ROS production and elastin synthesis) was evaluated morphologically and biochemically. Moreover, the protein profile was investigated by 2-DE and differentially expressed proteins were identified by MS. Serum withdrawal caused cell shrinkage but did not significantly modify the total cell number. ROS production, as evaluated by the dihydroethidium (DH2) probe, was increased after serum deprivation, whereas elastin synthesis, measured by a colorimetric method, was markedly reduced in the absence of serum. By proteome analysis, 41 proteins appeared to significantly change their expression, the great majority of protein changes were related to the cytoskeleton, the stress response and the glycolytic pathway. Data indicate that human dermal fibroblasts in primary cell culture can adapt themselves to environmental changes, without significantly altering cell viability, at least after a few days of treatment, even though serum withdrawal represents a stress condition capable to increase ROS production, to influence cell metabolism and to interfere with cell behaviour, favouring the expression of several age-related features.
Ključne besede:Dermal fibroblasts / Primary cell culture / ROS production / Serum withdrawal
Leto izida:2008
Št. strani:66-82
Številčenje:8
PID:20.500.12556/RUNG-4663-ef5d1e98-1a19-1437-d28b-68f25a6fbb13 Novo okno
COBISS.SI-ID:5425659 Novo okno
NUK URN:URN:SI:UNG:REP:UXJGPMJW
Datum objave v RUNG:22.07.2019
Število ogledov:3575
Število prenosov:0
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Gradivo je del revije

Naslov:Proteomics
Leto izida:2008

Licence

Licenca:CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
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Začetek licenciranja:19.07.2019

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