| Naslov: | Comparison of ex vivo and in vitro human fibroblast ageing models. |
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| Avtorji: | Boraldi, Federica (Avtor)
Annovi, Giulia (Avtor)
Tiozzo, Roberta (Avtor)
Sommer, Pascal (Avtor)
Quaglino, Daniela (Avtor) |
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| Datoteke: | Gradivo nima datotek. Gradivo je morda fizično dosegljivo v knjižnici fakultete, zalogo lahko preverite v COBISS-u.  |
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| Jezik: | Angleški jezik |
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| Vrsta gradiva: | Delo ni kategorizirano (r6) |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: | UNG - Univerza v Novi Gorici |
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| Opis: | Several studies have analyzed modulation of gene expression during physiological ageing with interesting, but often contradictory results, depending on the model used. In the present report we compare age-related metabolic and synthetic parameters in human dermal fibroblasts (HDF) isolated from young and old subjects (ex vivo ageing model) and cultured from early up to late cumulative population doublings (CPD) (in vitro ageing model) in order to distinguish changes induced in vivo by the aged environment and maintained in vitro, from those associated with cell senescence and progressive CPD. Results demonstrate that fibroblasts from aged donors, already at early CPD, exhibit an impaired redox balance, highlighting the importance of this parameter during ageing, even in the presence of standard environmental conditions, which are considered optimal for cell growth. By contrast, several proteins, as those related to heat shock response, or involved in endoplasmic reticulum and membrane trafficking, appeared differentially expressed only during in vitro ageing, suggesting that, at high CPD, the whole cell machinery becomes permanently altered. Finally, given the importance of the elastic component for a long-lasting connective tissue structural and functional compliance, this study focuses also on elastin and fibulin-5 synthesis and deposition, demonstrating a close relationship between fibulin-5 and ageing. |
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| Ključne besede: | Ageing
Fibroblast
Connective tissue
Oxidative stress
Protein expression
Elastin |
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| Leto izida: | 2010 |
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| Št. strani: | 625-635 |
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| Številčenje: | 131 |
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| COBISS_ID: | 5441019 |
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| URN: | URN:SI:UNG:REP:GJIOVNFF |
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| DOI: | 10.1016/j.mad.2010.08.008 |
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| Licenca: | 
To delo je dosegljivo pod licenco Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna |
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| Število ogledov: | 1952 |
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| Število prenosov: | 0 |
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| Metapodatki: |     |
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| Področja: | Gradivo ni uvrščeno v področja. |
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| | | | Skupna ocena: | (0 glasov) |
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| Vaša ocena: | Ocenjevanje je dovoljeno samo prijavljenim uporabnikom. |
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