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Naslov:Effects of high relative humidity and dry purging on VOCs obtained during breath sampling on common sorbent tubes
Avtorji:ID Wilkinson, Maxim, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United Kingdom (Avtor)
ID White, Iain R., Laboratory for Environmental and Life Sciences, University of Nova Gorica, Vipavska 13, Nova Gorica SI-5000, Slovenia (Avtor)
ID Goodacre, Roy, Department of Biochemistry, Institute of Integrative Biology, University of Liverpool, Biosciences Building, Crown Street, Liverpool L69 7ZB, United Kingdom (Avtor)
ID Nijsen, Tamara, Philips Research, Philips, Eindhoven, The Netherlands (Avtor)
ID Fowler, Stephen, Manchester Academic Health Science Centre and NIHR Biomedical Research Centre, Manchester University Hospitals NHS Foundation Trust, Manchester, United Kingdom (Avtor)
Datoteke:.pdf Wilkinson_2020_J._Breath_Res._14_046006.pdf (1,18 MB)
MD5: 313FC92EE111840170BBD9278191E16C
 
Jezik:Angleški jezik
Vrsta gradiva:Delo ni kategorizirano
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:UNG - Univerza v Novi Gorici
Opis:Offline breath analysis by thermal desorption gas chromatography mass spectrometry (TD-GC-MS) requires the use of sorbent traps to concentrate and store volatile compounds. The selection of which sorbent to use and best practices for managing high relative humidity are important considerations to allow for reproducible, untargeted, biomarker discovery in water saturated breath samples. This work aims to assess three commonly used sorbent materials for their use in breath volatile sampling and determine how the high relative humidity inherent in such samples effects the capture of volatile compounds of interest. TenaxGR, TenaxTA/Carbograph1TD and TenaxTA/Carbograph5TD tubes were selected as they are the most commonly used sorbents in the breath sampling literature. The recovery of 29 compounds in a standard mix loaded using high humidity gas was tested for each sorbent and compared to loading in dry gas. Water retention and dry purge rates were determined for each sorbent for 500 ml and 1000 ml breath collections. Finally, breath samples were collected simultaneously on to each sorbent type using the ReCIVA and analysed by TD-GC-MS. All three sorbents exhibited acceptable reproducibility when loaded with the standard mix in dry gas (RSD < 10%). Loading the standard mix in humid gas led to reduced recovery of compounds based on their chemical properties. Dry purging performance for each sorbent material was assessed and was shown to be 1.14, 1.13 and 0.89 mg H2O min−1 for TenaxGR, TenaxTA/Carbograph1TD and TenaxTA/Carbograph5TD respectively when flushed with 50 ml min−1 of N2. A comparison of breath profiles on different sorbents showed differences in background artefacts (sulfur dioxide, cyclopenten-1-one and 3-nonene) and endogenous breath compounds (2-methyl-furan and furfural). This work demonstrates that high relative humidity during sampling reduces the ability of sorbent tubes to capture volatile compounds and could impact method detection limits during breath sampling. Sufficient water to impair accurate analysis was retained on all tubes. Minimal differences were observed between sorbent materials when used to sample breath, however, suggestions are provided for sorbent selection for future studies.
Ključne besede:VOCs, Breath sampling, ReCIVA
Leto izida:2020
Št. strani:10
Številčenje:14
PID:20.500.12556/RUNG-5676 Novo okno
COBISS.SI-ID:23652099 Novo okno
DOI:10.1088/1752-7163/ab7e17 Novo okno
NUK URN:URN:SI:UNG:REP:LNTTDGJJ
Datum objave v RUNG:27.07.2020
Število ogledov:3029
Število prenosov:112
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
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Gradivo je del revije

Naslov:Journal of Breath Research
Skrajšan naslov:JBR
Založnik:IOPPublishingLtd
Leto izida:2020
ISSN:1752-7155

Licence

Licenca:CC BY-NC-SA 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Deljenje pod enakimi pogoji 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by-nc-sa/4.0/deed.sl
Opis:Licenca Creative Commons, ki prepoveduje komercialno uporabo in zahteva, da uporabnik predelana dela objavi z enako licenco.
Začetek licenciranja:27.07.2020

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