| Naslov: | 1,8-dihydroxy naphthalene (DHN) - melanin confers tolerance to cadmium in isolates of melanised dark septate endophytes |
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| Avtorji: | ID Potisek, Mateja (Avtor)
ID Likar, Matevž (Avtor)
ID Vogel-Mikuš, Katarina (Avtor)
ID Arčon, Iztok (Avtor)
ID Grdadolnik, Jože (Avtor)
ID Regvar, Marjana (Avtor) |
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| Datoteke: |
Gradivo nima datotek, ki so prostodostopne za javnost. Gradivo je morda fizično dosegljivo v knjižnici fakultete, zalogo lahko preverite v COBISS-u.  |
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| Jezik: | Angleški jezik |
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| Vrsta gradiva: | Neznano |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: | UNG - Univerza v Novi Gorici
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| Opis: | The contribution of 1,8-dihydroxy naphthalene (DHN) melanin to cadmium (Cd) tolerance in two dark septate
endophytes (DSE) of the genus Cadophora with different melanin content was investigated in vitro. The DSE
isolate Cad#148 with higher melanin content showed higher tolerance to Cd than the less melanised Cad#149.
Melanin synthesis was significantly reduced by Cd in both isolates with uninhibited melanin synthesis, in a dosedependent
manner. Inhibition of melanin synthesis by tricyclazole reduced the relative growth of Cad#148
exposed to Cd and did not affect Cad#149. Cd accumulation was not altered by tricyclazole in the two isolates,
but it increased catalase and reduced glutathione reductase activity in more melanised Cad#148, indicating
higher stress levels. In contrast, in Cad#149 the enzyme activity was less affected by tricyclazole, indicating a
more pronounced role of melanin-independent Cd tolerance mechanisms. Cd ligand environment in fungal
mycelia was analysed by extended EXAFS (X-ray absorption fine structure). It revealed that Cd was mainly bound
to O- and S-ligands, including hydroxyl, carboxyl, phosphate and thiol groups. A similar proportion of S- and Oligands
(~35% and ~65%) were found in both isolates with uninhibited melanin synthesis. Among O-ligands
two types with Cd-O-C- and Cd-O-P- coordination were identified. Tricyclazole altered Cd-O- ligand environment
in both fungal isolates by reducing the proportion of Cd-O-C- and increasing the proportion of Cd-O-P coordination.
DHN-melanin, among other tolerance mechanisms, significantly contributes to Cd tolerance in more
melanised DSE fungi by immobilising Cd to hydroxyl groups and maintaining the integrity of the fungal cell wall. |
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| Ključne besede: | DSE, melanin, Cd tolerance, inhibitor tricyclazole, antioxidant enzymes, EXAFS |
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| Leto izida: | 2021 |
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| Št. strani: | str. 1-9 |
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| Številčenje: | Vol. 222, 222 |
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| PID: | 20.500.12556/RUNG-6646 |
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| COBISS.SI-ID: | 70067203 |
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| UDK: | 577 |
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| ISSN pri članku: | 0147-6513 |
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| DOI: | 10.1016/j.ecoenv.2021.112493 |
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| NUK URN: | URN:SI:UNG:REP:9QTOEXOO |
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| Datum objave v RUNG: | 13.07.2021 |
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| Število ogledov: | 2899 |
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| Število prenosov: | 0 |
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| Metapodatki: |    |
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