Repozitorij Univerze v Novi Gorici

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Naslov:Microbial volatiles as diagnostic biomarkers of bacterial lung infection in mechanically ventilated patients
Avtorji:ID Ahmed, Waqar M, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, UK (Avtor)
ID Fenn, Dominic, Department of respiratory medicine, Amsterdam UMC location AMC, University of Amsterdam, Amsterdam, the Netherlands (Avtor)
ID White, Iain R., Laboratory for Environmental and Life Science, University of Nova Gorica, Nova Gorica, Slovenia (Avtor)
ID Dixon, Breanna, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, UK (Avtor)
ID Nijsen, Tamara M E, Philips Research, Philips B.V., Eindhoven, the Netherlands (Avtor)
ID Knobel, Hugo H, Eurofins Materials Science Netherlands BV, High Tech Campus, Eindhoven, the Netherlands (Avtor)
ID Brinkman, Paul, Department of respiratory medicine, Amsterdam UMC location AMC, University of Amsterdam, Amsterdam, the Netherlands (Avtor)
ID van Oort, Pouline M P, Department of Anaesthesiology, Amsterdam UMC location VUmc, Amsterdam, the Netherlands (Avtor)
ID Schultz, Marcus J, Intensive Care, Amsterdam UMC location AMC, Amsterdam, the Netherlands (Avtor)
ID Dark, Paul, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, UK (Avtor)
ID Goodacre, Royston, Centre for Metabolomics Research, Department of Biochemistry and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, UK (Avtor)
ID Felton, Timothy, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, UK (Avtor)
ID Bos, Lieuwe D J, Department of respiratory medicine, Amsterdam UMC location AMC, University of Amsterdam, Amsterdam, the Netherlands (Avtor)
ID Fowler, Stephen J., Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, UK (Avtor)
Datoteke: Gradivo nima datotek, ki so prostodostopne za javnost. Gradivo je morda fizično dosegljivo v knjižnici fakultete, zalogo lahko preverite v COBISS-u. Povezava se odpre v novem oknu
Jezik:Angleški jezik
Vrsta gradiva:Delo ni kategorizirano
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:UNG - Univerza v Novi Gorici
Opis:Background Early and accurate recognition of respiratory pathogens is crucial to prevent increased risk of mortality in critically ill patients. Microbial-derived volatile organic compounds (mVOCs) in exhaled breath could be used as non-invasive biomarkers of infection to support clinical diagnosis. Methods In this study, we investigated the diagnostic potential of in vitro confirmed mVOCs in the exhaled breath of patients under mechanically ventilation from the BreathDx study. Samples were analysed by thermal desorption-gas chromatography-mass spectrometry (TD-GC-MS). Results Pathogens from bronchoalveolar lavage (BAL) cultures were identified in 45/89 patients and S. aureus was the most commonly identified pathogen (n = 15). Out of 19 mVOCs detected in the in vitro culture headspace of four common respiratory pathogens (Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli), 14 were found in exhaled breath samples. Higher concentrations of two mVOCs were found in the exhaled breath of patients infected with S. aureus compared to those without (3-methylbutanal p < 0.01. AUROC = 0.81-0.87 and 3-methylbutanoic acid p = 0.01. AUROC = 0.79-0.80). In addition, bacteria identified from BAL cultures which are known to metabolise tryptophan (Escherichia coli, Klebsiella oxytoca and Haemophilus influenzae) were grouped and found to produce higher concentrations of indole compared to breath samples with culture-negative (p = 0.034) and other pathogen-positive (p = 0.049) samples. Conclusions This study demonstrates the capability of using mVOCs to detect the presence of specific pathogen groups with potential to support clinical diagnosis. Although not all mVOCs were found in patient samples within this small pilot study, further targeted and qualitative investigation is warranted using multi-centre clinical studies.
Ključne besede:Breath, VOCs, infection, respiratory pathogens, VAP
Verzija publikacije:Recenzirani rokopis
Leto izida:2022
Št. strani:23
Številčenje:ciac859
PID:20.500.12556/RUNG-7758 Novo okno
COBISS.SI-ID:130984195 Novo okno
DOI:10.1093/cid/ciac859 Novo okno
NUK URN:URN:SI:UNG:REP:ZWUI9CG4
Datum objave v RUNG:28.11.2022
Število ogledov:1443
Število prenosov:0
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
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Gradivo je del revije

Naslov:Clinical Infectious Diseases
Skrajšan naslov:Clin. Infect. Dis.
Založnik:Oxford University Press
Leto izida:2022
ISSN:1058-4838

Licence

Licenca:CC BY-NC-SA 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Deljenje pod enakimi pogoji 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by-nc-sa/4.0/deed.sl
Opis:Licenca Creative Commons, ki prepoveduje komercialno uporabo in zahteva, da uporabnik predelana dela objavi z enako licenco.
Začetek licenciranja:27.11.2022

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