Repozitorij Univerze v Novi Gorici

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Naslov:In vitro tumor hypoxia imaging with fluorescent covalent organic frameworks
Avtorji:ID Skorjanc, Tina (Avtor)
ID Shetty, Dinesh (Avtor)
ID Makuc, Damjan (Avtor)
ID Mali, Gregor (Avtor)
ID Bergant Marušič, Martina (Avtor)
ID Valant, Matjaž (Avtor)
Datoteke:URL https://skd2023.chem-soc.si/wp-content/uploads/2023/08/SKD-2023-Programska-knjizica-110823-3.pdf
 
Jezik:Slovenski jezik
Vrsta gradiva:Delo ni kategorizirano
Tipologija:1.12 - Objavljeni povzetek znanstvenega prispevka na konferenci
Organizacija:UNG - Univerza v Novi Gorici
Opis:Hypoxia refers to a condition where cells and tissues experience low, inadequate levels of O2. While healthy tissues are typically supplied with sufficient O2 (normoxia), cancerous tissues commonly face hypoxia due to the tumor’s extraordinarily high demand for oxygen. Various fluorescent small-molecule probes have been designed for selective detection of hypoxia in living cells, but few nanomaterials have been investigated for this type of bioimaging. Herein, we prepare a fluorescent covalent organic framework (COF) with β-ketoenamine linkages and post-synthetically modify it to conjugate hypoxia-sensitive nitroimidazole moieties into its pores (NI-COF). Stacks of sheets in NI-COF observed under electron microscopy were exfoliated by ultrasonication, and dynamic light scattering measurements confirmed particle size of less than 200 nm. Thus-prepared material exhibited good stability in physiological conditions and low cytotoxicity in in vitro experiments. NI-COF also showed useful fluorescence properties with an emission peak at 490 nm (λex = 420 nm) at both neutral and mildly acidic pH levels that are characteristic of tumor tissues. Encouraged by the favorable properties of the material, we incubated HeLa cells pre-treated in either hypoxic or normoxic conditions with NI-COF. Fluorescence microscopy images demonstrated that the material was preferentially taken up by hypoxic cells, which showed higher fluorescence signal in their interior than cells cultured under normoxia conditions. It is anticipated that this study will stimulate further developments of COFs for imaging various biological conditions.
Ključne besede:hypoxia, fluorescence, covalent organic frameworks, imaging, tumor cells
Leto izida:2023
PID:20.500.12556/RUNG-8438 Novo okno
COBISS.SI-ID:164896003 Novo okno
NUK URN:URN:SI:UNG:REP:UNKHHIIL
Datum objave v RUNG:19.09.2023
Število ogledov:981
Število prenosov:4
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Gradivo je del monografije

Naslov:Slovene Chemical Days 2023
Kraj izida:Portorož
Leto izida:2023
Prireditelj konference:Slovensko kemijsko društvo

Gradivo je financirano iz projekta

Financer:EC - European Commission
Program financ.:H2020
Številka projekta:101038091
Naslov:Novel COF-based sensors for detecting organic agents in water
Akronim:COFsensor

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