| Naslov: | Enhancing optical biosensing : comparing two physical treatments for GPTES chemical functionalization of cyclo-olefin copolymer foil |
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| Avtorji: | ID Ressel, Barbara (Avtor)
ID Urbančič, Jurij (Avtor)
ID Beltrami, Marco (Avtor)
ID Betz-Güttner, Erik (Avtor)
ID Cepek, Cinzia (Avtor)
ID Conti, Martina (Avtor)
ID Farooq, Ayesha (Avtor)
ID Melpignano, Patrizia (Avtor) |
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| Datoteke: |  https://www.sciencedirect.com/science/article/pii/S2468023024008216?via%3Dihub
 1-s2.0-S2468023024008216-main.pdf (1,88 MB)
MD5: 8004DB782FF2D1F323A65D72B0194ECD
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| Jezik: | Angleški jezik |
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| Vrsta gradiva: | Neznano |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: | UNG - Univerza v Novi Gorici
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| Opis: | Cyclo-olefin-copolymer (COC) transparent films are currently the best choice for micro-fluidic bio-sensors for point-of-care diagnostic applications using optical signal detection. However, while the optical and mechanical properties of this polymer are extremely good, the adhesion of the bio-probes on this surface is not optimal, due to its chemical structure, that presents only saturated carbon bonds. The deposition of organo-silane molecules on the COC surface is one of the most effective ways to overcome this problem. But, for the surface functionalization, a surface physical treatment is necessary before the chemical modification of the COC surface. In this paper a comparison of the effectiveness of two different physical treatments, oxygen plasma and UV-ozone, is reported. In particular, the exposure time of the UV-Ozone treatment has been selected to avoid the problem of auto-fluorescence of the modified COC surface, that was observed also for relatively short UV exposure (around 10 minutes). An investigation of the reactive radicals created on the surface after the physical treatments and the following chemical modification with the organo-silane molecule (GPTES) has been performed using X-ray photoemission spectroscopy. The surface energy and morphology of the films have been also measured by contact angle and optical profilometry. Finally, the bio-probes adhesion performances of the COC surfaces obtained with the two physical treatments and the chemical modification were tested in a fluorescence-based assay, using an organic light emission diode to excite the fluorescence. We observed that the UV-ozone treatment allows to obtain a siloxane network with some reactive epoxy radicals on the COC surface, however, their quantity and distribution are less important and homogeneous than in the oxygen plasma treated surfaces. |
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| Ključne besede: | cyclo-olefin-copolymers, organo-silane, oxygen plasma, UV-ozone, XPS, OLED |
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| Status publikacije: | V tisku |
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| Verzija publikacije: | Recenzirani rokopis |
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| Datum objave: | 01.01.2024 |
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| Leto izida: | 2024 |
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| Št. strani: | str. 1-24 |
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| Številčenje: | Vol. , [article no.] ǂ104663 |
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| PID: | 20.500.12556/RUNG-9153  |
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| COBISS.SI-ID: | 199943683 |
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| UDK: | 53 |
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| ISSN pri članku: | 2468-0230 |
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| DOI: | 10.1016/j.surfin.2024.104663 |
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| NUK URN: | URN:SI:UNG:REP:MISFNVQX |
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| Datum objave v RUNG: | 26.06.2024 |
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| Število ogledov: | 95 |
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| Število prenosov: | 0 |
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| Metapodatki: |     |
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