Naslov: | Functional characterisation of surfactant protein A as a novel prophylactic means against oncogenic HPV infections |
---|
Avtorji: | ID Carse, Sinead (Avtor) ID Reid, Tim (Avtor) ID Madsen, Jens (Avtor) ID Clark, Howard (Avtor) ID Kirjakulov, Artur (Avtor) ID Bergant Marušič, Martina (Avtor) ID Schäfer, Georgia (Avtor) |
Datoteke: | https://www.mdpi.com/1422-0067/25/14/7712
ijms-25-07712-published2024Sinead.pdf (6,10 MB) MD5: 8A0D589A3E3A66D6742CA96470700FF1
https://www.mdpi.com/1422-0067/25/14/7712/pdf
|
---|
Jezik: | Angleški jezik |
---|
Vrsta gradiva: | Neznano |
---|
Tipologija: | 1.01 - Izvirni znanstveni članek |
---|
Organizacija: | UNG - Univerza v Novi Gorici
|
---|
Opis: | Human papillomavirus (HPV) infection poses a significant health challenge, particularly in low- and middle-income countries (LMIC), where limited healthcare access and awareness hinder vaccine accessibility. To identify alternative HPV targeting interventions, we previously reported on surfactant protein A (SP-A) as a novel molecule capable of recognising HPV16 pseudovirions (HPV16-PsVs) and reducing infection in a murine cervicovaginal HPV challenge model. Building on these findings, our current study aimed to assess SP-A’s suitability as a broad-spectrum HPV-targeting molecule and its impact on innate immune responses. We demonstrate SP-A’s ability to agglutinate and opsonise multiple oncogenic HPV-PsVs types, enhancing their uptake and clearance by RAW264.7 murine macrophages and THP-1 human-derived immune cells. The SP-A opsonisation of HPV not only led to increased lysosomal accumulation in macrophages and HaCaT keratinocytes but also resulted in a decreased infection of HaCaT cells, which was further decreased when co-cultured with innate immune cells. An analysis of human innate immune cell cytokine profiles revealed a significant inflammatory response upon SP-A exposure, potentially contributing to the overall inhibition of HPV infection. These results highlight the multi-layered impact of SP-A on HPV, innate immune cells and keratinocytes and lay the basis for the development of alternative prophylactic interventions against diverse HPV types. |
---|
Ključne besede: | surfactant protein A, SP-A, human papillomavirus, HPV, pseudovirus, cervical cancer, HPV prophylactic, low- and middle-income countries, LMIC |
---|
Status publikacije: | Objavljeno |
---|
Verzija publikacije: | Objavljena publikacija |
---|
Datum objave: | 01.01.2024 |
---|
Leto izida: | 2024 |
---|
Št. strani: | str. 1-22 |
---|
Številčenje: | Vol. 25, issue 14, [article no.] 7712 |
---|
PID: | 20.500.12556/RUNG-9196 ![Povezava se odpre v novem oknu Novo okno](teme/rungDev/img/NovoOkno.png) |
---|
COBISS.SI-ID: | 202458371 ![Povezava se odpre v novem oknu Novo okno](teme/rungDev/img/NovoOkno.png) |
---|
UDK: | 54 |
---|
ISSN pri članku: | 1422-0067 |
---|
eISSN: | 1422-0067 |
---|
DOI: | 10.3390/ijms25147712 ![Povezava se odpre v novem oknu Novo okno](teme/rungDev/img/NovoOkno.png) |
---|
NUK URN: | URN:SI:UNG:REP:Y1ZZ8AGC |
---|
Datum objave v RUNG: | 22.07.2024 |
---|
Število ogledov: | 531 |
---|
Število prenosov: | 7 |
---|
Metapodatki: | ![Izvoz v DC-RDF DC-RDF](teme/rungDev/img/icon_dc_rdf.gif) |
---|
:
|
Kopiraj citat |
---|
| | | Skupna ocena: | (0 glasov) |
---|
Vaša ocena: | Ocenjevanje je dovoljeno samo prijavljenim uporabnikom. |
---|
Objavi na: | ![Bookmark and Share](teme/rungDev/img/addtoany.png) |
---|
Postavite miškin kazalec na naslov za izpis povzetka. Klik na naslov izpiše
podrobnosti ali sproži prenos. |