31. The filter loading effect by ambient aerosols in filter absorption photometers depends on the mixing state of the sampled particlesLuka Drinovec, Asta Gregorič, Peter Zotter, Robert Wolf, Emily Anne Bruns, Andre S.H. Prevot, Jean-Eudes Petit, Olivier Favez, JEAN SCIARE, Ian J. Arnold, Rajan K. Chakrabarty, Hans Moosmüller, Agnes Fülöp-Miller, Griša Močnik, 2016, original scientific article Keywords: black carbon, mixing state, Aethalometer, filter-loading effect
Published in RUNG: 02.11.2016; Views: 5671; Downloads: 281
 Full text (2,07 MB) |
32. NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodiesSandrine Moutel, Nicolas Bery, Virginie Bernard, Laura Keller, Emilie Lemesre, Ario De Marco, Laetitia Ligat, Jean-Christophe Rain, Gilles Fevre, Aurelien Olichon, Franck Perez, 2016, original scientific article Abstract: In vitro selection of antibodies allows to obtain highly functional binders, rapidly and at
lower cost. Here, we describe the first fully synthetic phage display library of humanized llama
single domain antibody (NaLi-H1: Nanobody Library Humanized 1). Based on a humanized synthetic
single domain antibody (hs2dAb) scaffold optimized for intracellular stability, the highly diverse
library provides high affinity binders without animal immunization. NaLi-H1 was screened following
several selection schemes against various targets (Fluorescent proteins, actin, tubulin, p53, HP1).
Conformation antibodies against active RHO GTPase were also obtained. Selected hs2dAb were
used in various immunoassays and were often found to be functional intrabodies, enabling tracking
or inhibition of endogenous targets. Functionalization of intrabodies allowed specific protein
knockdown in living cells. Finally, direct selection against the surface of tumor cells produced
hs2dAb directed against tumor-specific antigens further highlighting the potential use of this
library for therapeutic applications.
Keywords: nanobodies, synthetic phage display library, in vitro panning
Published in RUNG: 17.08.2016; Views: 5360; Downloads: 243
Full text (4,32 MB) |
33. The effect of bilingualism on the processing of scalar implicaturesPenka Stateva, Sara Andreetta, Ludivine Dupuy, Anne Cheylus, Viviane Déprez, Jean-Baptiste van der Henst, Jacques Jayez, Arthur Stepanov, Anne Reboul, 2016, published scientific conference contribution abstract Abstract: Scalar implicatures have been extensively investigated in the experimental literature, but almost exclusively in monolingual speakers. Very little research has been conducted on the pragmatic abilities of multilingual populations, including early bilinguals to L2 learners, a gap the current study aims to remedy.
Keywords: L2 learning and early bilingualism, comprehension of scalar implicatures
Published in RUNG: 22.04.2016; Views: 5055; Downloads: 0
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