21. Categorical approach of normal injective and torsionable hypermodulesHashem Bordbar, 2024, objavljeni povzetek znanstvenega prispevka na konferenci Opis: Inspired by the characterization of injective objects in category theory,
our main purpose in this research is to investigate the relationships of di-
visible and injective hypermodules with torsion elements and torsionable
hypermodules with a category approach. These days, it is extremely
tempt- ing to do the research in an Abelian category, so we discuss these
topics in an Abelian category. For a Krasner R-hypermodule M, we ex-
tend the definition of a zero-divisor element of R to a zero-divisor ele-
ment of R over M, and then the definition of divisible R-hypermodule is
introduced. Be- sides, the torsion element and torsionable hypermodule
are introduced and we show that every torsionable R-hypermodule M is a
normal injective, where R is a commutative hyperring.
Ključne besede: hypermodules, hyperrings, normal injective, normal projective
Objavljeno v RUNG: 18.06.2024; Ogledov: 197; Prenosov: 0
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22. Native agarose gels and contact blotting as means to optimize the protocols for the formation of antigen-ligand complexesClaudia D'Ercole, Ario De Marco, 2024, objavljeni povzetek znanstvenega prispevka na konferenci Opis: Background. Protein complexes provide valuable biological information but can be difficult to handle [1]. Therefore, technical advancements designed to improve their manipulation are al-ways useful.
Methods. We investigated the opportunity to exploit native agarose gels and the contact bot method for the transfer of native proteins to membranes as means for optimizing the conditions for obtaining stable complexes [2]. As a simple model of protein-protein interactions, an anti-gen-ligand complex was used in which both proteins were fused to reporters.
Results. At each step, it was possible to visualize both the antigen, fused to a fluorescent pro-tein, and the ligand, fused to a monomeric ascorbate peroxidase (APEX) and, as such, a way to tune the protocol. The conditions for the complex formation were adapted by modifying the buffer conditions, the concentration of the proteins and of the cross-linkers.
Conclusions. The procedure is rapid, inexpensive, and the several detection opportunities al-lows for both the monitoring of complex stability and the preservation of the functionality of its components, which is critical for understanding their biomedical implications and supporting drug discovery. The overall protocol represents a handy alternative to gel filtration, uses very standard and ubiquitous equipment, and can be implemented rapidly and without specific train-ing.
References:
[1] O. Puig, F. Caspary, G. Rigaut, B. Rutz, E. Bouveret, E. Bragado-Nilsson, M. Wilm, B. Séraphin. The tandem affinity purification (TAP) method: a general procedure of protein complex purification. Methods. (San Diego, Calif.), 24(3), 218–229
[2] C. Sakuma, M. Nakagawa, Y. Tomioka, T. Maruyama, K. Entzminger, J.K. Fleming, T. Shibata, Y. Kurosawa, C.J. Okumura, T. Arakawa, T. Akuta. Western blotting of native proteins from agarose gels. Biotechniques. 2022 May;72(5):207-218
Ključne besede: protein complexes, contact blotting, native agarose gels, protein interaction
Objavljeno v RUNG: 17.06.2024; Ogledov: 227; Prenosov: 0
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