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Ključne besede: clitics, enclitics, Slavic, syntax, Slovenian, Slovak, Czech, Bulgarian, Macedonian, BCMS, Polish, clitic cluster, stress
Objavljeno v RUNG: 31.05.2024; Ogledov: 107; Prenosov: 0
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Opis: Forest environments are exposed to multiple stressful factors of both abiotic and biotic nature which may lead to their massive decline [1]. Understanding the molecular mechanisms of specific stress conditions and monitoring the fluctuations of reliable forest plant biomarkers with affordable methods would be instrumental for assessing stress levels over the time. Ascorbate peroxidase (APX) represents a suitable plant biomarker. APX is a hydrogen peroxide-scavenging enzyme the critical role of which has been described in several plants, both herbaceous and woody. Its activity generally increases under oxidative stress during which its peroxide detoxifying function is part of the wider ascorbate-glutathione cycle [2]. The development of reagents to detect such fluctuations would help the evaluation of plant physiological conditions. In this study, nanobodies (Nbs) targeting APX have been identified. Nbs correspond to the variable domain of heavy chain-only antibodies derived from camelids. They are small (15 kDa), stable, and can be easily produced in bacteria fused to different protein tags according to the downstream applications [3]. After their isolation by biopanning against soluble APX, they have been produced and underwent a biophysical characterization in combination with their antigen (APX-Nb complex) to identify the best binders in terms of stability and affinity. The protein complex characterization was supported by Instruct-ERIC and mainly performed at the BIOCEV institute of Prague. Data from Mass Photometry and Dynamic Light scattering evidenced the formation of the protein complexes, whereas the preliminary data of Hydrogen-Deuterium Exchange Mass Spectrometry, performed with the aim of identifying the residues involved in the paratope/epitope interface, were insufficient to clarify the issue and rather suggested that the interaction has low affinity. This indication was then confirmed by ELISA assay. The combination of multiple methods allowed a comprehensive sample characterization which will require further structural analyses to provide a complete picture of the APX-Nb complex. [1] G. Marie. B. C. M. B. C. Walters, “Forest decline and tree mortality in a southeastern Ohio oak-hickory forest,” Ohio Journal of Science , vol. 97, 1997. [2] O. Chew, J. Whelan, and A. H. Millar, “Molecular Definition of the Ascorbate-Glutathione Cycle in Arabidopsis Mitochondria Reveals Dual Targeting of Antioxidant Defenses in Plants,” Journal of Biological Chemistry, vol. 278, no. 47, 2003, doi: 10.1074/jbc.M307525200. [3] S. Muyldermans, “A guide to: generation and design of nanobodies,” FEBS J, vol. 288, no. 7, pp. 2084–2102, Apr. 2021, doi: 10.1111/febs.15515.
Ključne besede: nanobody, ascorbate peroxidase, plant stress, protein complex, biophysical methodologies
Objavljeno v RUNG: 31.05.2024; Ogledov: 115; Prenosov: 0
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Opis: This paper is about two pilot studies conducted in 2022 that aimed to develop a model for preserving and archiving new media art work in the context of a research project on the sustainable digital preservation of new media art that is being co-hosted by the Museum of Modern Art in Ljubljana. As the works of art selected for the study by Slovenian new media art pioneers Vuk Ćosić (2000) and Srečo Dragan (2005) were technically obsolete or non-functional by the time of the study, the question of how to bring the artworks back into existence and what components of each artwork to include in the collection and preservation process constituted one aspect of our research. But this process of reconstruction also raised questions about how the preservation of media art is reshaping the practice of archiving within an institution whose holdings were, until recently, largely in traditional mediums. An interdisciplinary approach addressed the problem from different points of view, involving the practitioners, experts from art-history, museology, computer science, media theory and intellectual property rights.
Ključne besede: digital cultural heritage, new media art preservation, new media art archives
Objavljeno v RUNG: 28.05.2024; Ogledov: 179; Prenosov: 0
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Ključne besede: strong lensing, LSST
Objavljeno v RUNG: 22.05.2024; Ogledov: 239; Prenosov: 1
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Opis: Differential Scanning Calorimetry (DSC) is a regular and powerful tool to measure the specific heat profile of various materials. In order to connect the measured profile to the properties of a particular protein, a model is required to fit. We discuss here the application of an exact two-state formula with its approximation and process the DSC experimental data on protein folding in water. The approximate formula relies on the smallness of the transition interval, which is different for each protein. With an example of the set of 33 different proteins, we show the practical validity of the approximation and the equivalence of exact and approximate two-state formulas for processing DSC data.
Ključne besede: protein folding, differential scanning calorimetry, heat capacity, two-state model, Hawley model
Objavljeno v RUNG: 21.05.2024; Ogledov: 278; Prenosov: 3
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