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Opis: V dveh .csv datotekah so zbrani vsi eksperimentalni podatki, ki smo jih uporabili pri pisanju članka "O razvrščanju nikalnice v goriških govorih".
Ključne besede: eksperimentalni podatki, zanikanje, slovenščina
Objavljeno v RUNG: 22.04.2025; Ogledov: 127; Prenosov: 2
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Opis: Marine ecosystems are constantly influenced by anthropogenic pressures, including inputs of nutrients, pollutants, and allochthonous microorganisms. These inputs can disrupt the dynamics of marine microbiomes, which encompass the majority of biomass and highest diversity of all life forms, controlling most biogeochemical cycles in the ocean realm. Despite their importance for public safety and potential influence on marine ecosystems functioning, knowledge of the impact of these perturbations on coastal microbiome dynamics remains in its infancy. The research objectives of this dissertation were to: (1) characterize the composition of the coastal microbiome in anthropogenically impacted coastal ecosystem; (2) investigate the genetic potential of selected microorganisms for pathogenicity and ecological adaptations; and (3) explore the effects of wastewater on the dynamics and functioning of the coastal microbiome, as well as its implications for the biogeochemical state of the ecosystem. To address the first aim, we conducted a year-round in situ survey of the pelagic microbiome within anthropogenically impacted coastal ecosystem, focusing on the seasonal and spatial dynamics of traditional and alternative faecal bacterial indicators. We used a culture-independent approach combined with 16S rRNA amplicon sequencing, which overcome limitations of culture-based methods. This analysis revealed that the microbiome was primarily structured by seasonal changes, regardless of proximity to pollution sources. The statistical tool and oceanographic model we applied indicated that riverine water serves as a key vector for introducing allochthonous microbes. This study underscores the importance of molecular approaches combined with statistical and oceanographic modelling for advancing environmental health assessments and detecting microbial indicators. To address the second aim, we applied whole-genome sequencing to assess the pathogenic potential and genomic features of selected Vibrio isolates. During the analysis, we identified cross-contamination in one isolate, which presented an opportunity to evaluate the effectiveness of bioinformatics workflows for contaminant removal and genome recovery. We reconstructed high-quality genomes of one Vibrio isolate from both axenic and contaminated cultures. Genomic analyses revealed that this isolate belongs to a sub-lineage of Vibrio campbellii associated with diseases in marine organisms. Moreover, this genome harboured a novel Vibrio plasmid linked to bacterial defense mechanisms and horizontal gene transfer, potentially offering a competitive advantage to this putative pathogen. This study highlights the utility of WGS and advanced bioinformatics in overcoming challenges posed by non-axenic cultures and provides new insights into the genomic characteristics of V. campbellii. To address the third aim, we conducted a short-term microcosm experiment simulating wastewater discharge into coastal seawater, testing two types of wastewaters: (a) unfiltered, containing nutrients, pollutants, and allochthonous microbes, and (b) pre-filtered, retaining only nutrients and pollutants. Our results showed that wastewater, significantly increased nutrient levels (dissolved organic carbon, ammonium, orthophosphate). Using a multi-omics approach with measurements of microbial metabolic activity, we found that nutrient enrichment significantly influenced bacterial metabolism. This was evidenced by enriched protein profiles and increased leucine aminopeptidase and olease activity, indicating bacterial degradation of complex proteins and lipids. At the same time, the phosphate input resulted in a decreased alkaline phosphatase activity, with important implications for phosphorus cycling. Overall, wastewater primarily induced functional shifts in coastal microbiomes, highlighting the resilience and functional redundancy of coastal microbial communities and hence the biogeochemical processes they operate.
Ključne besede: coastal microbiome, anthropogenic impact, wastewater pollution, bacterial community dynamics, allochthonous microorganisms, potential pathogens, Vibrio campbellii, multi-omics, whole-genome assembly, dissertations
Objavljeno v RUNG: 16.04.2025; Ogledov: 229; Prenosov: 2
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Ključne besede: pripovednost v liriki, lirski cikel
Objavljeno v RUNG: 15.04.2025; Ogledov: 162; Prenosov: 0
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Opis: Enticed by the promise of larger sales and better access to customers, consumer goods compa- nies (CGCs) are increasingly looking to evade traditional retailers and reach their customers directly–with direct-to-customer (DTC) policy. DTC trend has emerged to have major im- pact on logistics operations and distribution channels. It oers significant opportunities for CGCs and wholesale brands to better control their supply chain network by circumventing the middlemen or retailers. However, to do so, CGCs may need to develop their omni-channel strategies and fortify their supply chains parameters, such as fulfillment, inventory flow, and goods distribution. This may give rise to changes in the supply chain network at all strategic, tactical and operational levels. Motivated by recent interests in DTC trend, this master thesis considers the time-evolving supply chain system of an international healthcare company with preordained configuration. The input is bottleneck part of the company’s distribution network and involves 20% ≠ 25% of its total market. A mixed-integer linear programming (MILP) multiperiod optimization model is developed aiming to make tactical decisions for designing the distribution network, or more specifically, for determining the best strategy for distributing the products from manufacturing plant to primary distribution center and/or regional distribution centers and from them to customers. The company has got one manufacturing site (Mfg), one primary distribution center (PDP) and three dierent regional distribution centers (RDPs) worldwide, and the customers can be supplied from dierent plants with various transportation modes on dierent costs and lead times. The company’s motivation is to investigate the possibility of reduction in distribution costs by in-time supplying most of their demand directly from the plants. The model selects the best option for each customer by making trade-os among criteria involving distribution costs and lead times. Due to the seasonal variability and to account the market fluctuability, the model considers the full time horizon of one year. The model is analyzed and developed step by step, and its functionality is demonstrated by conducting experiments on the distribution network from our case study. In addition, the case study distribution network topology is utilized to create random instances with random parameters and the model is also evaluated on these instances. The computational experiments on instances show that the model finds good quality solutions, and demonstrate that significant cost reduction and modality improvement can be achieved in the distribution network. Using one-year actual data, it has been shown that the ratio of direct shipments could substantially improve. However, there may be many factors that can impact the results, such as short-term decisions at operational level (like scheduling) as well as demand fluctuability, taxes, business rules etc. Based on the results and managerial considerations, some possible extensions and final recommendations for distribution chain are oered. Furthermore, an extensive sensitivity analysis is conducted to show the eect of the model’s parameters on its performance. The sensitivity analysis employs a set of data from our case study and randomly generated data to highlight certain features of the model and provide some insights regarding its behaviour.
Ključne besede: optimization, mixed-integer linear programming, supply chain, distribution network, sensitivity analysis
Objavljeno v RUNG: 14.04.2025; Ogledov: 231; Prenosov: 2
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Opis: Prvi vsebinski del zbornika se nanaša na projekt Muzej tovorništva in prevozništva, v katerem avtor in avtorice prispevkov spregovorijo o procesu pridobivanja sredstev, o izvedbi dosedanjega dela, utemeljujejo vizijo obnove mitnice ter pojasnijo vsebinski oris muzejske postavitve. V drugem delu zbornika avtorji in avtorice predstavijo svoje raziskave vezane na arheološko preteklost Tabora pri Vrabčah, jamski turizem skozi čas, kraško zidovje v okolici Rodika in na družbeno vlogo ohranjanja suhih zidov in kulturne krajine.
Ključne besede: Senožeče, Muzej tovorništva in prevozništva, Kras, kulturna krajina, suhi zid, zgodovina, arheologija, jamski turizem, Divača
Objavljeno v RUNG: 14.04.2025; Ogledov: 222; Prenosov: 0
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