11. Laser ablation-single-particle-inductively coupled plasma mass spectrometry as a multimodality bioimaging tool in nano-based omicsDino Metarapi, Johannes Teun van Elteren, Martin Šala, Katarina Vogel-Mikuš, Iztok Arčon, Vid Simon Šelih, Mitja Kolar, Samo B. Hočevar, 2021, original scientific article Abstract: Advanced data processing and visualization techniques were applied to laser ablation-single particleinductively
coupled plasma mass spectrometry (LA-sp-ICPMS) data obtained from imaging of roots of
sunflower (Helianthus annuus L.) which were exposed to ionic silver (Ag+). Highly multiplexed images with
tissue level resolution were generated, showing the uptake and transformation of Ag+ to silver
nanoparticles (AgNPs) in whole root cross-sections, and revealing that the size of biosynthesized AgNPs is
predisposed by the reducing power of particular root compartments. Various Ag+ and AgNP visualization
strategies are shown with special emphasis on spatially resolved information associated with the number
and spherical-equivalent size of individual AgNPs in user-selected root cross-sectional regions, including
statistical analysis of the AgNP distribution. Normally, this multimodality information can only be obtained
by combining the results of techniques from several complementary platforms. Keywords: Laser ablation, Ag XANES, bioimaging Published in RUNG: 03.06.2021; Views: 2133; Downloads: 50 Link to full text This document has many files! More... |
12. Delo jezikovnega asistenta za slovenščino v okviru manjšinskega srednješolskega izobraževanja na TržaškemKatarina Gomboc Čeh, 2020, independent scientific component part or a chapter in a monograph Abstract: V šolskem letu 2018/2019 je bilo prvič razpisano mesto jezikovnega asistenta za slovenščino na višjih srednjih šolah s slovenskim učnim jezikom v Trstu. Članek izhaja iz izkušenj avtorice, jezikovne asistentke za slovenščino v šolskih letih 2018/2019 in 2019/2020. V prvem delu utemeljuje vlogo jezikovnega asistenta za slovenščino v okviru manjšinskega srednješolskega izobraževanja na Tržaškem, nato pa na podlagi orisa sociolingvistične situacije ponuja nastavke didaktičnih pristopov in možnosti za razvijanje sporazumevalne zmožnosti v slovenskem knjižnem jeziku na višjih srednjih šolah s slovenskim učnim jezikom na Tržaškem. Keywords: slovenščina, pouk slovenščine, manjšinsko šolstvo, slovenska šola v Italiji, učni jezik, jezikovni asistent, sporazumevalna zmožnost Published in RUNG: 16.03.2021; Views: 2201; Downloads: 0 This document has many files! More... |
13. Marketinška raziskava o potrebah usposabljanj s področja vinogradništva, vinarstva, promocije in trženja vina na področju primorske vinorodne dežele : projektno delo z gospodarstvom in negospodarstvom v lokalnem in regionalnem okolju - Po kreativni poti do znanjaVanesa Klinec, Tereza Valentinčič, Kristina Cotič, Katarina Marković, Natallia Person, Yue Ma, final research report Keywords: projekti, marketinške raziskave, potrebe, izobraževanje, vinogradništvo, vinarstvo Published in RUNG: 23.02.2021; Views: 2578; Downloads: 0 This document has many files! More... |
14. Temporal and spatial patterns of zinc and iron accumulation during barley (Hordeum vulgare L.) grain development. Journal of agricultural and food chemistry.Amelie Detterbeck, Paula Pongrac, Daniel Persson, Katarina Vogel-Mikuš, Mitja Kelemen, Primož Vaupetič, Primož Pelicon, Iztok Arčon, Søren Husted, Jan Kofod Shjoerring, Stephan Clemens, 2020, original scientific article Abstract: Breeding and engineering of biofortified crops will benefit from a better understanding of bottlenecks controlling
micronutrient loading within the seeds. However, few studies have addressed the changes in micronutrient concentrations,
localization, and speciation occurring over time. Therefore, we studied spatial patterns of zinc and iron accumulation during grain
development in two barley lines with contrasting grain zinc concentrations. Microparticle-induced-X-ray emission and laser ablationinductively
coupled plasma mass spectrometry were used to determine tissue-specific accumulation of zinc, iron, phosphorus, and
sulfur. Differences in zinc accumulation between the lines were most evident in the endosperm and aleurone. A gradual decrease in
zinc concentrations from the aleurone to the underlying endosperm was observed, while iron and phosphorus concentrations
decreased sharply. Iron co-localized with phosphorus in the aleurone, whereas zinc co-localized with sulfur in the sub-aleurone. We
hypothesize that differences in grain zinc are largely explained by the endosperm storage capacity. Engineering attempts should be
targeted accordingly. Keywords: barley (Hordeum vulgare L.), biofortification, grain development, grain loading, LA-ICP-MS, μ-PIXE Published in RUNG: 20.10.2020; Views: 2794; Downloads: 0 This document has many files! More... |
15. Pogovor brez koncaKatarina Blažič, 2020, undergraduate thesis Abstract: Za svojo diplomsko nalogo sem se odločila opisati proces nastajanja animiranega
filma, narejenega na temo slepih in slabovidnih. Namen animiranega filma je približati
svet slepih in slabovidnih ljudem, ki vidimo. V svetu, v katerem živimo, je velik del
našega počutja odvisen od zunanjih dejavnikov (socialna omrežja, različne reklame
ipd.). Pomembno je zavedanje, da ni vse, kar vidimo, zlato. Animirani film se ukvarja
z različnimi vprašanji kot so: kako se slep človek spoprijema z različnimi tematikami,
kako se sooča z vsakdanjimi opravili, razmerji z drugimi ljudmi in na splošno, kako se
spoprijema s svetom in okolico in to bom tudi opisala podrobneje v diplomski nalogi.
Animirani film sem želela približati slepim in slabovidnim s tem, da sem dala največji
poudarek zvoku, zvočnim učinkom in spremljajočemu besedilu animiranega filma.
Izbrala sem barve, ki so računalniško izračunane in prilagojene za barvno slepe ljudi. Z
uporabo preprostih oblik v animaciji sem hotela doseči to, da slaboviden gledalec lažje
sledi animaciji in da ga hkrati spomni na to, kako bi lahko slepa oseba narisala različne
objekte. V diplomskem delu bom opisala torej celoten proces izdelave animiranega
filma in uvod v temo slepih in slabovidni z opisom umetnikov in kratkem povzetku
kako slepi rišejo in kaj si mislijo o tej temi. Povzela bom vse, kar je zgoraj navedeno
in podrobno opisala nastanek animiranega filma. Keywords: animirani film, slepi, slepota, slabovidni Published in RUNG: 27.05.2020; Views: 3575; Downloads: 112 Full text (4,57 MB) This document has many files! More... |
16. Arabidopsis halleri shows hyperbioindicator behaviour for Pb and leaf Pb accumulation spatially separated from ZnStephan Höreth, Paula Pongrac, Marta Debeljak, Katarina Vogel-Mikuš, Matic Pečovnik, Primož Vavpetič, Iztok Arčon, original scientific article Abstract: Lead (Pb) ranks among the most problematic environmental pollutants. Background contamination of soils is nearly ubiquitous, yet plant Pb accumulation is barely understood. In a survey covering 165 European populations of the metallophyte Arabidopsis halleri, several field samples had indicated Pb hyperaccumulation, offering a chance to dissect plant Pb accumulation.
Accumulation of Pb was analysed in A. halleri individuals from contrasting habitats under controlled conditions to rule out aerial deposition as a source of apparent Pb accumulation. Several elemental imaging techniques were employed to study the spatial distribution and ligand environment of Pb.
Regardless of genetic background, A. halleri individuals showed higher shoot Pb accumulation than A. thaliana. However, dose–response curves revealed indicator rather than hyperaccumulator behaviour. Xylem sap data and elemental imaging unequivocally demonstrated the in planta mobility of Pb. Highest Pb concentrations were found in epidermal and vascular tissues. Distribution of Pb was distinct from that of the hyperaccumulated metal zinc. Most Pb was bound by oxygen ligands in bidentate coordination.
A. halleri accumulates Pb whenever soil conditions render Pb phytoavailable. Considerable Pb accumulation under such circumstances, even in leaves of A. thaliana, strongly suggests that Pb can enter food webs and may pose a food safety risk. Keywords: Pb accumulation, XANES, EXAFS, Arabidopsis halleri Published in RUNG: 16.01.2020; Views: 3099; Downloads: 0 This document has many files! More... |
17. Mineral element composition in grain of awned and awnletted wheat (Triticum aestivum L.) cultivars tissue-specific iron speciation and phytate and non-phytate ligand ratioPaula Pongrac, Iztok Arčon, Hiram Castillo Michel, Katarina Vogel-Mikuš, 2020, original scientific article Abstract: In wheat (Triticum aestivum L.), the awns—the bristle-like structures extending from
lemmas—are photosynthetically active. Compared to awned cultivars, awnletted cultivars produce
more grains per unit area and per spike, resulting in significant reduction in grain size, but their
mineral element composition remains unstudied. Nine awned and 11 awnletted cultivars were
grown simultaneously in the field. With no difference in 1000-grain weight, a larger calcium and
manganese—but smaller iron (Fe) concentrations—were found in whole grain of awned than in
awnletted cultivars. Micro X-ray absorption near edge structure analysis of different tissues of
frozen-hydrated grain cross-sections revealed that differences in total Fe concentration were not
accompanied by differences in Fe speciation (64% of Fe existed as ferric and 36% as ferrous species) or
Fe ligands (53% were phytate and 47% were non-phytate ligands). In contrast, there was a distinct
tissue-specificity with pericarp containing the largest proportion (86%) of ferric species and nucellar
projection (49%) the smallest. Phytate ligand was predominant in aleurone, scutellum and embryo
(72%, 70%, and 56%, respectively), while nucellar projection and pericarp contained only non-phytate
ligands. Assuming Fe bioavailability depends on Fe ligands, we conclude that Fe bioavailability from
wheat grain is tissue specific. Keywords: biofortification, phytate, iron, awn, X-ray fluorescence, X-ray absorption spectrometry, phosphorus, sulphur, nicotianamine Published in RUNG: 16.01.2020; Views: 2922; Downloads: 0 This document has many files! More... |
18. Localization, ligand environment, bioavailability and toxicity of mercury in Boletus spp. and Scutiger pes-caprae mushroomsAnja Kavčič, Klemen Mikuš, Marta Debeljak, Johannes Teun van Elteren, Iztok Arčon, Alojz Kodre, Peter Kump, Andreas-Germanos Karydas, Alessandro Migliori, Mateusz Czyzycki, Katarina Vogel-Mikuš, 2019, original scientific article Abstract: This study provides information on mercury (Hg) localization, speciation and ligand environment in edible mushrooms: Boletus edulis, B. aereus and Scutiger pes-caprae collected at non-polluted and Hg polluted sites, by LA-ICP-MS, SR-μ-XRF and Hg L3-edge XANES and EXAFS. Mushrooms (especially young ones) collected at Hg polluted sites can contain more than 100 μg Hg g−1 of dry mass. Imaging of the element distribution shows that Hg accumulates mainly in the spore-forming part (hymenium) of the cap. Removal of hymenium before consumption can eliminate more than 50% of accumulated Hg.
Mercury is mainly coordinated to di-thiols (43–82%), followed by di-selenols (13–35%) and tetra-thiols (12–20%). Mercury bioavailability, as determined by feeding the mushrooms to Spanish slugs (known metal bioindicators owing to accumulation of metals in their digestive gland), ranged from 4% (S. pes-caprae) to 30% (B. aereus), and decreased with increasing selenium (Se) levels in the mushrooms. Elevated Hg levels in mushrooms fed to the slugs induced toxic effects, but these effects were counteracted with increasing Se concentrations in the mushrooms, pointing to a protective role of Se against Hg toxicity through HgSe complexation. Nevertheless, consumption of the studied mushroom species from Hg polluted sites should be avoided. Keywords: edible mushrooms, HgSe complex, imaging of elemental distribution, LA-ICP-MS, alpha-XRF, XAS Published in RUNG: 24.10.2019; Views: 4611; Downloads: 0 This document has many files! More... |
19. Recent advances in 2D imaging of element distribution in Plants by focused beam techniquesKatarina Vogel-Mikuš, Johannes Teun van Elteren, Marjana Regvar, Jitrin Chaiprapa, Boštjan Jenčič, Iztok Arčon, Alojz Kodre, Peter Kump, Anja Kavčič, Mitja Kelemen, Dino Metarapi, Marijan Nečemer, Primož Vavpetič, Primož Pelicon, Paula Pongrac, 2019, independent scientific component part or a chapter in a monograph Keywords: 2D Imiging, XRF, XANES, synchrotron light Published in RUNG: 05.09.2019; Views: 3276; Downloads: 0 This document has many files! More... |
20. Evaluation of policy and technical factors for the protection of karst aquifersKatarina Kosič Ficco, 2019, doctoral dissertation Abstract: Karst aquifer protection remains inchoate. National and international legally-binding mechanisms rarely consider implementation of karst-specific measures for their evaluation and protection. Although developing suitable protection measures for karst aquifers is challenging because their variable character hampers implementation of generalized methods, it is not impossible. As presented in this dissertation, consideration of scientific aspects and inclusion of karst-specific evaluation methods is of great importance. However, the additional inclusion of socio-political aspects is also crucial for advances in karst aquifer protection. Analyses of current European Union and United States of America policies performed in this study have shown that they can provide valuable input regarding the topic. It is also clear that accounting for the interests of affected entities, such as policy-makers, stakeholders and the public, is critical. Satisfying their needs, and assuring their understanding of karst aquifers, can ease and improve implementations of adopted measures. By recognizing these aspects, an interdisciplinary framework for karst aquifer protection was developed, and is presented in this study. The so-called K-framework, developed as a checklist, assures implementation of necessary steps for comprehensive karst aquifer evaluation and management. It further defines how each entity can contribute to the process by providing their knowledge, and allocates responsibilities of each sphere. An important attribute of the proposed framework is that it can be used on varying karst terrains and for various impacts that might threaten karst aquifers. Additionally, it is cost- and time-efficient, while still considering important aspects of karst and insuring the inclusion of important affected parties. Keywords: aquifer, groundwater, karst, protection, environmental legislation, confined animal feeding operation, vulnerability mapping Published in RUNG: 17.06.2019; Views: 3851; Downloads: 144 Full text (11,63 MB) |