51. DOLOČANJE ACETILHOLINESTERAZE V HUMANIH KRVNIH VZORCIHTjaša Birsa, 2018, master's thesis Abstract: V strokovni literaturi zasledimo encim acetilholinesterazo (AChE) v različnih raziskavah s področja okoljevarstva, medicine in veterine. Zmanjšana encimska aktivnost je lahko indikator onesnaženosti okolja z organofosfatnimi in karbamatnimi pesticidi, policikličnimi aromatskimi ogljikovodiki ter težkimi kovinami. Spremembe v encimski aktivnosti AChE pa so tudi biološki označevalec nekaterih nevrodegenerativnih bolezni pri človeku.
V človeškem telesu se encim AChE primarno nahaja v živčnem sistemu, najdemo pa ga tudi v krvi, zlasti v membranah eritrocitov. V krvi je v večji meri prisoten tudi encim butirilholinesteraza (BChE), ki ravno tako spada v družino holinesteraz. Naš cilj je bil razviti metodo, ki bi omogočala specifično merjenje encimske aktivnosti AChE v okviru celotnih holinesteraz v krvnem serumu.
Za merjenje encimske aktivnosti smo uporabili Ellmanovo metodo. Količino obarvanih produktov reakcije smo določali s spektroskopijo s toplotnimi lečami TLS in z UV-VIS spektrofotometrijo. Z različnimi koncentracijami čistega encima AChE smo najprej določili mejo zaznavnosti obeh metod (LOD). Detekcija s TLS je bila bolj občutljiva, saj je bil LOD 3,13-krat manjši kot pri detekciji z UV-VIS. Potem smo izmerili encimsko aktivnost celotnih holinesteraz AChE in BChE v serumskih vzorcih zdravih ljudi. Izračunana encimska aktivnost holinesteraz se je v analiziranih vzorcih gibala med 3004 mU mL-1 in 5698 mU mL-1. Po dodatku specifičnega inhibitorja BChE se je celotna encimska aktivnost znižala za najmanj 60 %, kar kaže, da večina holinesteraz v krvnem serumu pripada BChE. Preizkusili smo tudi metodo enojne imunske precipitacije, vendar se je le-ta izkazala za neuspešno pri ločevanju posameznih holinesteraz iz serumskih vzorcev.
Na podlagi naših rezultatov smo prišli do zaključka, da je za merjenje encimske aktivnosti posameznih holinesteraz AChE in BChE v krvnem serumu najprimernejša uporaba specifičnih inhibitorjev, vendar bi bilo to metodo potrebno še dodatno optimizirati. Keywords: acetilholinesteraza, krvni serum, nevrodegenerativne bolezni, spektroskopija s toplotnimi lečami, UV-VIS spektrofotometrija, imunska precipitacija. Published in RUNG: 07.11.2018; Views: 6183; Downloads: 175 Full text (1,65 MB) |
52. Vpliv proteinov APOBEC na infekcijo z virusi HPVTjaša Lojpur, 2018, master's thesis Abstract: Humani virusi papiloma (HPV) so povzročitelji najpogostejše spolno prenosljive okužbe, povezani pa so tudi z razvojem raka materničnega vratu. Proteini APOBEC so DNA/RNA editirajoči encimi, ki igrajo ključno vlogo pri prirojenih protivirusnih imunskih odzivih in so vpleteni tako v infekcijo s HPV kot tudi v razvoj rakavih obolenj pri dolgotrajni okužbi s HPV. Glavni cilj magistrskega dela je bil proučiti protivirusno delovanje proteinov APOBEC in določiti tudi ožji nabor proteinov APOBEC, vključenih v protivirusni odziv pri infekciji s HPV. Vpliv proteinov APOBEC na primarno infekcijo smo ugotavljali z uporabo psevdovirusnih delcev (PsV) HPV, v plašču katerih je bil vgrajen reporterski gen za luciferazo. Stopnjo infekcije virusov HPV v gostiteljskih celičnih linijah, transficiranih s proteini hAPOBEC, smo določali posredno preko encimske aktivnosti luciferaze. Ugotovili smo, da izražanje večine proteinov hAPOBEC zniža infektivnost PsV HPV. Izmed proučevanih proteinov hAPOBEC so imeli najmočnejši protivirusni odziv hAID, hA1, hA2, hA3A, hA3B, hA3C in hA3DE, saj so zmanjšali infektivnost PsV HPV-16 za več kot 50 %. Čeprav smo protivirusni učinek proteinov hAPOBEC ugotovili tudi pri dveh kožnih tipih, HPV-2 in HPV-5, je bil le ta značilno višji pri visokorizičnem sluzničnem tipu HPV-16. Mehanizme delovanja proteinov hAPOBEC smo ugotavljali s katalitskimi in nekatalitskimi mutantami proteinov hA3A (E72A) in hA3B (E255Q in E68Q), ki so za razliko od divjih tipov, infektivnost HPV-16 povečale. Mikroskopska analiza znotrajcelične lokalizacije izbranih proteinov hAPOBEC med infekcijo s HPV-16 pa je pokazala, da se 24 ur po infekciji zgodi premik deleža proteina hA3A iz citoplazme v jedro, kar bi lahko vplivalo na potek infekcije. V magistrskem delu smo potrdili prvotno hipotezo, da proteini APOBEC sodelujejo pri protivirusnem odzivu virusov HPV, njihov protivirusni učinek pa je odvisen tako od vrste proteina APOBEC kot tudi od tipa virusa HPV. Keywords: HPV, proteini APOBEC, protivirusni odziv, infektivnost, znotrajcelična lokalizacija Published in RUNG: 05.11.2018; Views: 5515; Downloads: 164 Full text (1,64 MB) |
53. Arsenic in natural waters: hydrogeochemistry characterization and toxicity effectsDoroteja Gošar, 2018, master's thesis Abstract: Arsenic contamination in natural water is a worldwide problem and a major health concern. In master thesis hydrogeochemistry and toxicity effects of natural waters rich in arsenic were studied. The main objective of the master thesis was to evaluate As pollution of the Freixeda stream and groundwater in abandoned Freixeda gold mine area in NE Portugal near Mirandela city (41.413767 N 7.103562 W) and compare it with the data from previous studies. Further on, toxicological evaluation of selected water samples was performed in the in vitro system of human cell line Caco-2.
Chemical analyses of sampled water samples with use of different modelling sofware show that groundwater have higher sulphate and bicarbonate values than surface water, which could be the reason for As desorption and higher As values in groundwater. Water-rock interaction promotes reduction and dissolution of sulphide minerals and in reductive environments dissolution of secondary Fe minerals releases adsorbed As into solution.
Toxicological testing on human cells included cytotoxicity assay, genotoxicity assay and production of reactive oxigene species (ROS). Genotoxicity was only modestly affected by a short-term exposure to As-contaminated water samples, however, higher concentrations of As in real samples lead to higher level of oxidative stress and decreased cell viability. Exposure of cells to pure As(III) solution show clear concentration dependent decrease in cell metabolism and viability, strong genotoxicity and increased ROS generation.
Considering the worldwide extent of As contamination in natural waters and ability of intestinal epithelium to reduce the potential harmful effects of As, more studies evaluating the human intestinal permeability for As should be done in the future. Keywords: Water quality, arsenic, hydrogeochemistry, toxicity, Caco-2 cell line Published in RUNG: 26.09.2018; Views: 5470; Downloads: 197 Full text (3,76 MB) |
54. How to react to the necessity of a sustainable animal production? The EcoLamb projectTanja Peric, Martina Bergant Marušič, Ario De Marco, Petra Makorič, Sinan Ogun, 2018, published scientific conference contribution abstract Abstract: EcoLamb assesses the sustainability of diverse European sheep production systems focusing on the ecological footprint, animal welfare aspects and nutrition value of lamb meat. The outcomes of these assessments will be used to understand the potential future barriers that limit the innovative capacity and development of the sector and the opportunities that may provide a future market niche against competitive products from other global markets. Farm solutions that incorporate consumer expectations for animal welfare and meat quality will enhance the competitiveness of Europe's lamb meat sector. The project engages trans-national research and industry stakeholders from 6 countries made up of Germany, Italy, Portugal, Slovenia, Spain and Turkey to analyse on 20 case study farms resource-efficient, competitive and low-carbon lamb production models. Direct linkage between animal welfare, meat quality and pharmaceutical use will also be determined using innovative Precision Farming techniques. The project will produce a tool-box of recommendations for productive sheep farm management, supply chain and marketing on how to improve the acceptability of lamb meat by consumers. Thus, the multidisciplinary approach and the multi-actor involvement of the EU sheep sector will assist in re-designing critical aspects to increase society acceptance and the place of lamb meat in future diets and the outcomes of the project will be used by stakeholders to promote changes in farm management, marketing and processing of meat from sheep. Additionally, results will be used by farm consultants, farmer groups and policy officers to re-design consulting approaches and plan new initiatives to make all aspects of the European sheep industry more sustainable. Keywords: sustainable, animal production, lamb, sheep, Jezersko-Solčavska, EcoLamb Published in RUNG: 05.09.2018; Views: 3698; Downloads: 0 This document has many files! More... |
55. Rapid and Sensitive Determination of ChE Activity in Blood PlasmaAndrej Jerkič, Tjaša Birsa, Mojca Žorž, Dorota Korte, Martina Bergant Marušič, Mladen Franko, 2018, published scientific conference contribution abstract Keywords: flow injection analysis, thermal lens spectrometric detection, acetylcholinesterase, butyrylcholinesterase, enzyme activity Published in RUNG: 16.07.2018; Views: 4386; Downloads: 0 This document has many files! More... |
56. Procjena genotoksičnog potencijala uzoraka aerosola iz TuzleMaida Šljivić Husejnović, Dalila Imamović, Ermina Cilović, Aida Smajlović, Nahida Srabović, Martina Bergant Marušič, 2018, published scientific conference contribution Keywords: aerozagađenje, genotoksičnost, zdravlje ljudi, aerosol, Tuzla Published in RUNG: 30.03.2018; Views: 3863; Downloads: 0 This document has many files! More... |
57. Univerza v Novi Gorici, Spremljanje, ugotavljanje in zagotavljanje kakovosti, Poročilo za študijsko leto 2016/2017Martina Bergant Marušič, Blaž Belec, Lorena Butinar, IMRE CIKAJLO, Saša Dobričič, Renata Kop, Martin Knez, Boštjan Potokar, Branka Mozetič Vodopivec, Egon Pavlica, Samo Stanič, Tea Stibilj Nemec, Arthur Stepanov, Veronika Piccinini, Anika Velišček, Peter Purg, Aljaž Rener, Nives Štefančič, Ana Toroš, Tanja Urbančič, Matjaž Valant, Aleš Vaupotič, Sabina Zelinšček, 2017, treatise, preliminary study, study Keywords: zagotavljanje kakovosti, visoko šolstvo, univerza Published in RUNG: 05.02.2018; Views: 4324; Downloads: 0 This document has many files! More... |
58. Univerza v Novi Gorici, Spremljanje, ugotavljanje in zagotavljanje kakovosti, Poročilo za študijsko leto 2015/2016Martina Bergant Marušič, Lorena Butinar, IMRE CIKAJLO, Saša Dobričič, Renata Kop, Urška Lavrenčič Štangar, Martin Knez, Boštjan Potokar, Branka Mozetič Vodopivec, Egon Pavlica, Samo Stanič, Tea Stibilj Nemec, Arthur Stepanov, Veronika Piccinini, Tamara Podveršič, Peter Purg, Aljaž Rener, Nives Štefančič, Ana Toroš, Tanja Urbančič, Vanesa Valentinčič, Aleš Vaupotič, Sabina Zelinšček, 2016, treatise, preliminary study, study Keywords: zagotavljanje kakovosti, visoko šolstvo, univerza Published in RUNG: 05.02.2018; Views: 4216; Downloads: 0 This document has many files! More... |
59. Univerza v Novi Gorici, Spremljanje, ugotavljanje in zagotavljanje kakovosti, Poročilo za študijsko leto 2014/2015Martina Bergant Marušič, Aleš Bogovič, Lorena Butinar, IMRE CIKAJLO, Saša Dobričič, Elsa Fabbretti, Renata Kop, Urška Lavrenčič Štangar, Martin Knez, Katja Mihurko, Branka Mozetič Vodopivec, Egon Pavlica, Samo Stanič, Tea Stibilj Nemec, Arthur Stepanov, Boštjan Potokar, Veronika Piccinini, Peter Purg, Aljaž Rener, Nives Štefančič, Ana Toroš, Tanja Urbančič, Vanesa Valentinčič, Aleš Vaupotič, Sabina Zelinšček, 2015, treatise, preliminary study, study Keywords: zagotavljanje kakovosti, visoko šolstvo, univerza Published in RUNG: 05.02.2018; Views: 4426; Downloads: 0 This document has many files! More... |
60. Univerza v Novi Gorici, Spremljanje, ugotavljanje in zagotavljanje kakovosti, Poročilo za študijsko leto 2013/2014Iztok Arčon, Martina Bergant Marušič, Aleš Bogovič, Lorena Butinar, Gvido Bratina, IMRE CIKAJLO, Saša Dobričić, Elsa Fabbretti, Mladen Franko, Renata Kop, Urška Lavrenčič Štangar, Martin Knez, Katja Mihurko, Jana Laganis, Branka Mozetič Vodopivec, Egon Pavlica, Ingrid Petrič, Samo Stanič, Tea Stibilj Nemec, Arthur Stepanov, Boštjan Potokar, Veronika Piccinini, Peter Purg, Aljaž Rener, Nives Štefančič, Ana Toroš, Tanja Urbančič, Vanesa Valentinčič, Aleš Vaupotič, Sabina Zelinšček, 2014, treatise, preliminary study, study Keywords: zagotavljanje kakovosti, visoko šolstvo, univerza Published in RUNG: 05.02.2018; Views: 4309; Downloads: 0 This document has many files! More... |