1. The spectrum of building block conformers sustains the biophysical properties of clinically-oriented self-assembling protein nanoparticlesEric Voltà-Durán, Julieta M. Sánchez, Hèctor López-Laguna, Eloi Parladé, Laura Sánchez-García, Alejandro Sánchez-Chardi, Ario De Marco, Ugutz Unzueta, Esther Vázquez, Antonio Villaverde, 2022, izvirni znanstveni članek Ključne besede: protein nanoparticles, nanobodies, protein self-assembling Objavljeno v RUNG: 15.02.2022; Ogledov: 1573; Prenosov: 36 Celotno besedilo (5,71 MB) |
2. Biofabrication of functional protein nanoparticles through simple His-tag engineeringHèctor López-Laguna, Julieta M. Sánchez, José Vicente Carratalá, Mauricio Rojas-Peña, Laura Sánchez-García, Eloi Parladé, Alejandro Sánchez-Chardi, Eric Voltà-Durán, Naroa Serna, Ario De Marco, 2021, izvirni znanstveni članek Ključne besede: nanobodies, protein nanoparticles, controlled aggregation Objavljeno v RUNG: 24.09.2021; Ogledov: 1872; Prenosov: 37 Povezava na celotno besedilo Gradivo ima več datotek! Več... |
3. Self-assembled nanobodies as selectively targeted, nanostructured, and multivalent materialsLaura Sánchez-García, Eric Voltà-Durán, Eloi Parladé, Elisa Mazzega, Alejandro Sánchez-Chardi, Naroa Serna, Hèctor López-Laguna, Mara Mitstorfer, Ugutz Unzueta, Esther Vázquez, Antonio Villaverde, Ario De Marco, 2021, pregledni znanstveni članek Ključne besede: nanobodies, nanoparticles, fluorescent reagents, protein self-assembling Objavljeno v RUNG: 30.06.2021; Ogledov: 2144; Prenosov: 0 Gradivo ima več datotek! Več... |
4. Simultaneous ternary extension of DNA catalyzed by a trimeric replicase assembled in vivoAlejandro Montón Silva, Fabio Lapenta, Alessandra Stefan, Fabrizio Dal Piaz, Alessandro Ceccarelli, Alessandro Perrone, Alejandro Hochkoeppler, 2015, izvirni znanstveni članek Opis: According to current models, dimeric DNA Polymerases coordinate the replication of DNA leading and lagging strands. However, it was recently shown that trimeric DNA Polymerases, assembled in vitro, replicate the lagging strand more efficiently than dimeric replicases. Here we show that the τ, α, ε, and θ subunits of Escherichia coli DNA Polymerase III can be assembled in vivo, yielding the trimeric τ3α3ε3θ3 complex. Further, we propose a molecular model of this complex, whose catalytic action was investigated using model DNA substrates. Our observations indicate that trimeric DNA replicases reduce the gap between leading and lagging strand synthesis. Ključne besede: Trimeric DNA Polymerase, E. coli, DNA Polymerase III, τ3α3ε3θ3 complex, Triple DNA extension Objavljeno v RUNG: 27.01.2021; Ogledov: 2041; Prenosov: 0 Gradivo ima več datotek! Več... |
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