21. Matrix Gla protein (MGP) is involved in elastic fiber calcification in the dermis of Pseudoxanthoma Elasticum (PXE) patients.Gheduzzi Dealba, Federica Boraldi, Annovi Giulia, Paolinelli Devincenzi Chiara, Schurgers Leon J, Vermeer Cees, Quaglino Daniela, Pasquali Ronchetti Ivonne, 2007, izvirni znanstveni članek Opis: Mature MGP (Matrix g-carboxyglutamic acid protein) is known to inhibit soft connective tissues calcification. We
investigated its possible involvement in pseudoxanthoma elasticum (PXE), a genetic disorder whose clinical manifestations
are due to mineralization of elastic fibers. PXE patients have lower serum concentration of total MGP compared to
controls (Po0.001). Antibodies specific for the noncarboxylated (Glu-MGP) and for the g-carboxylated (Gla-MGP) forms of
MGP were assayed on ultrathin sections of dermis from controls and PXE patients. Normal elastic fibers in controls and
patients were slightly positive for both forms of MGP, whereas Gla-MGP was more abundant within control’s than within
patient’s elastic fibers (Po0.001). In patients’ calcified elastic fibers, Glu-MGP intensively colocalized with mineral
precipitates, whereas Gla-MGP precisely localized at the mineralization front. Data suggest that MGP is present within
elastic fibers and is associated with calcification of dermal elastic fibers in PXE.
Ključne besede: calcification, dermal fibroblast, elastic fiber, human skin, MGP, pseudoxanthoma elasticum
Objavljeno v RUNG: 22.07.2019; Ogledov: 3264; Prenosov: 0
Gradivo ima več datotek! Več... |
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23. Cardiac stem cell aging and heart failureDaniela Cesselli, Aneta Aleksova, Elisa Mazzega, Angela Caragnano, Antonio Paolo Beltrami, 2017, pregledni znanstveni članek Opis: A side effect of the medical improvements of the last centuries is the progressive aging of the world population, which is estimated to reach the impressive number of 2 billion people with more than 65 years by 2050. As a consequence, age-related diseases, such as heart failure, will affect more and more patients in the next years. To understand the biological bases of these diseases will be a crucial task in order to find better treatments, and possibly slow age-related morbidity and mortality.
Cardiac stem cells have been at the center of a heated debate and their potential involvement in cardiac homeostasis has been questioned. In this review, we summarize evidence obtained by independent groups, on different animal models and humans, that strongly support the important role played by immature, cardiac resident cells in the cardioprotection against heart failure.
Ključne besede: Aging, Heart failure, Cardiomyocyte turnover, Stem cells, Cell senescence, cKit, Sca1, PDGFRα, Cardiospheres
Objavljeno v RUNG: 28.03.2017; Ogledov: 4481; Prenosov: 0
Gradivo ima več datotek! Več... |
24. Ex Vivo Molecular Rejuvenation Improves the Therapeutic Activity of Senescent Human Cardiac Stem Cells in a Mouse Model of Myocardial InfarctionElisa Avolio, Giuseppe Gianfranceschi, Angela Caragnano, Emmanouil Athanasakis, Rajesh Katare, Daniela Cesselli, Marco Meloni, Anita Palma, Arianna Barchiesi, Carlo Vascotto, Barbara Toffoletto, Elisa Mazzega, Nicoletta Finato, Giuseppe Aresu, Ugolino Livi, Costanza Emanueli, Giacinto Scoles, Carlo Alberto Beltrami, Paolo Madeddu, Antonio Paolo Beltrami, 2014, izvirni znanstveni članek Opis: Cardiac stem cells (CSC) from explanted decompensated hearts (E-CSC) are, with respect to those obtained from healthy donors (D-CSC), senescent and functionally impaired. We aimed to identify alterations in signaling pathways that are associated with CSC senescence. Additionally, we inves- tigated if pharmacological modulation of altered pathways can reduce CSC senescence in vitro and enhance their reparative ability in vivo. Measurement of secreted factors showed that E-CSC release larger amounts of proinflammatory cytokine IL1b compared with D-CSC. Using blocking antibodies, we verified that IL1b hampers the paracrine protective action of E-CSC on cardiomyo- cyte viability. IL1b acts intracranially inducing IKKb signaling, a mechanism that via nuclear factor-jB upregulates the expression of IL1b itself. Moreover, E-CSC show reduced levels of AMP protein kinase (AMPK) activating phosphorylation. This latter event, together with enhanced IKKb signaling, increases TORC1 activity, thereby impairing the autophagic flux and inhibiting the phos- phorylation of Akt and cAMP response element-binding protein. The combined use of rapamycin and resveratrol enhanced AMPK, thereby restoring downstream signaling and reducing IL1b secretion. These molecular corrections reduced E-CSC senescence, re-establishing their protective activity on cardiomyocytes. Moreover ex vivo treatment with rapamycin and resveratrol improved E-CSC capacity to induce cardiac repair upon injection in the mouse infarcted heart, leading to reduced cardiomyocyte senescence and apoptosis and increased abundance of endog- enous c-Kit1 CSC in the peri-infarct area. Molecular rejuvenation of patient-derived CSC by short pharmacologic conditioning boosts their in vivo reparative abilities. This approach might prove useful for refinement of CSC-based therapies.
Ključne besede: Stem cells, Myocardial infarction, Cellular senescence, Heart failure
Objavljeno v RUNG: 21.03.2017; Ogledov: 3991; Prenosov: 0
Gradivo ima več datotek! Več... |
25. In silico generation of peptides by replica exchange Monte Carlo: Docking-based optimization of maltose-binding-protein ligandsAnna Russo, Pasqualina Liana Scognamiglio, Rolando Pablo Hong Enriquez, Carlo Santambrogio, Rita Grandori, Daniela Marasco, Antonio Giordano, Giacinto Scoles, Sara Fortuna, 2015, izvirni znanstveni članek Opis: Short peptides can be designed in silico and synthesized through automated techniques, making them advantageous and versatile protein binders. A number of docking-based algorithms allow for a computational screening of peptides as binders. Here we developed ex-novo peptides targeting the maltose site of the Maltose Binding Protein, the prototypical system for the study of protein ligand recognition. We used a Monte Carlo based protocol, to computationally evolve a set of octapeptides starting from a polialanine sequence. We screened in silico the candidate peptides and characterized their binding abilities by surface plasmon resonance, fluorescence and electrospray ionization mass spectrometry assays. These experiments showed the designed binders to recognize their target with micromolar affinity. We finally discuss the obtained results in the light of further improvement in the ex-novo optimization of peptide based binders.
Ključne besede: peptides, docking, optimisation, computation, maltose binding protein, probe, ligand
Objavljeno v RUNG: 12.10.2016; Ogledov: 3958; Prenosov: 140
 Celotno besedilo (4,27 MB) |
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27. “LESSONS LEARNED” (PARTIAL TEST DEPLOYMENTS REPORT) : ADRIART PROJECTTomislav Brajnović,, Peter Purg, Daniela Brasil, Alessandro Bordina, Miljana Babić, 2014, druge monografije in druga zaključena dela Opis: The report consist of individual test-run reports by participating teachers and consortium-level observers that gathered qualitative and quantitative data through short interviews, surveys and QA questionnaires mostly with students, but also among themselves. A joint evaluation is delivered and a “lessons learned” compendium published, offered digitally through the project’s dissemination system (mostly newsletter and website-promotion), and spread locally through the teaching communities – also partly entering the professional article as “experience report” (see next deliverable). These reports and the “lessons learned” were discussed and confirmed in the below structure at the September 2013 meeting, organised by P4. Notably, a total of three sceintific or porfessional articles were (to be) published (as separate deliverables) refereing to the lessons learned in this project, refering to the summative experience of the project.
Ključne besede: Curriculum, module, course, study programme, degree, international, comparison, study, national, studio, MA
Objavljeno v RUNG: 05.07.2016; Ogledov: 4868; Prenosov: 215
 Povezava na celotno besedilo |
28. New Collaboration Forms in Site-specific Blended Courses Abroad: : Lessons Learned in the ADRIART.net Curriculum Development ProjectPeter Purg, Daniela Brasil, 2016, izvirni znanstveni članek Opis: A condensed programme of international courses with site-specific focus was developed as part of the collaborative art study programme Media Arts and Practices. Among higher-education partners from Italy, Austria, Croatia, and Slovenia the collaboration crossed the realms of new-media and contemporary art, film, animation, photography and scenography. Aiming at art pedagogy practitioners including art-school managers, who plan to develop or implement similar forms of intensive courses or programmes, the article discusses several key phenomena emerging among different stakeholders of the artistic or media-production education process. A plethora of research-and-development data, gathered along the three years of the collaborative study programme provision and intensive short-term course deployments, were condensed into lessons-learned that focus around the aspects of (blended) course design, interdisciplinary teaching and production methods, academic feedback and critique, as well as impact on local stakeholders. By shedding multi-layered light at site-specific art pedagogy along four short case-comparisons (from Graz, Komiža, Rijeka, and Venice), the article reflects an important trend in the arts—increasingly hybrid, multidisciplinary practices. It shows how social and (aesth)ethical change is well possible within a culturally reflected art pedagogy-cum-production setting that can materialize a collective and meaningful impact on a specific site, and its social tissue.
Ključne besede: Site-specific, Academic Experience Abroad, Collaboration
Objavljeno v RUNG: 23.05.2016; Ogledov: 4910; Prenosov: 0
Gradivo ima več datotek! Več... |
29. Development of Slow Control Boards for the Large Size Telescopes of the Cherenkov Telescope ArrayDaniela Hadasch, Andrej Filipčič, Gašper Kukec Mezek, Samo Stanič, Serguei Vorobiov, Lili Yang, Gabrijela Zaharijas, Danilo Zavrtanik, Marko Zavrtanik, objavljeni znanstveni prispevek na konferenci Ključne besede: Cherenkov Telescope Array (CTA), Large Size Telescopes (LST), imaging camera, slow control board (SCB)
Objavljeno v RUNG: 21.03.2016; Ogledov: 4316; Prenosov: 195
Celotno besedilo (4,05 MB) |
30. EFFECT OF TIMING OF LEAF REMOVAL ON YIELD PARAMETERS, GRAPE AND WINE QUALITY OF VITIS VINIFERA L. CV. 'SAUVIGNON BLANC' Daniela MARKOVICDaniela Markovic, 2016, diplomsko delo Opis: Z odstranjevanjem listov v predelu grozdov se lahko spremeni mikroklima grozdov, ki lahko izzove spremembe v sekundarnem metabolizmu vinske trte, ki so odvisne tudi od časa izvedbe ukrepa. Uravnavanje sinteze aromatskih komponent je zanimivo pri belih sortah kot je sorta 'Sauvignon blanc', kjer so metoksipirazini in tioli tisti, ki oblikujejo značilno aromo vina te sorte.
Eksperiment diplomske naloge smo zastavili na Oslavju (Gorica, Italija) v vinogradu sorte grozdja 'Sauvignon blanc' (Vitis vinifera L.). Želeli smo preveriti vpliva odstranjevanja listov v predelu grozdja pred in po cvetenju na količino in kakovost pridelka ter aromatske lastnosti grozdja in vina.
Količinski parametri pridelka so pokazali majhne vplive izvedenih ukrepov razlistanja; pozno razlistanje na količino pridelka ni vplivalo, v nasprotju z razlistanjem pred cvetenjem, kjer smo opazili rahlo zmanjšanje pridelka po trsu in mase grozdov. Tudi pri osnovnih zrelostnih parametrih smo opazili manjše spremembe, predvsem večje vsebnosti topne suhe snovi pri razlistanju pred cvetenjem.
Pri aromatskih komponentah smo zaznali vpliv razlistanja pred cvetenjem na količino prekurzorjev tiolov tako v grozdju kot tudi vinu; večjo vsebnost 4-merkapto-4-metilpentan-2-on (4MMP) in nižje koncentracije 3-merkaptoheksan-1-ol (3MH). S poznejšim odstranjevanjem listov smo zmanjšali koncentracije 4MMP, na vsebnost 3MH ta ukrep ni vplival.
Tudi s senzorično oceno vina smo potrdili pomembne vplive razlistanja pred in po cvetenju na določene note v aromi vina 'Sauvignon blanc'.
Leaf removal changes cluster microclimate, and thus modifications in the secondary metabolism are triggered, but differently as regard to the timing of application. In case of white grape varieties, the interest on the modification of aroma characteristics is searched, and in case of 'Sauvignon blanc', the shift in concentration of methoxypyrazines and thiols is responsible for the bouquet of the wines.
An experimental trial was set up in Oslavia (Gorizia, Italy) with the aim to evaluate how pre-flowering and post-flowering leaf removal applied on 'Sauvignon blanc' vines could change the aromatic occurrence in grapes and wines.
The yield parameters were slightly affected by the treatments of leaf removal; while late leaf removal did not impact on production, pre-flowering leaf removal slightly promoted a reduction on yield and cluster weight. Also basic maturation parameters were slightly changed, mainly higher soluble solids in case of pre-flowering leaf removal.
As regard aromatics, both thiol precursors in grapes and thiols in wines revealed to be shifted in case of pre-flowering leaf removal, with higher values of 4-mercapto-4-methyl-pentan-2-one and lower values of 3-mercaptohexan-1-ol. In case of late leaf removal lower concentration of 4MMP were revealed and similar of 3MH.
Also the degustation of the wines revealed some peculiarities of pre-flowering and late leaf removal treatments for some important sensorial notes of 'Sauvignon blanc' wines.
Ključne besede: Vitis vinifera L. cv. 'Sauvignon blanc', razlistanje pred cvetenjem, razlistanje po cvetenju, aromatske komponente, tioli, LC-MS
Vitis vinifera L. cv. 'Sauvignon blanc', pre-flowering leaf removal, post-flowering leaf removal, aromatic compounds, thiols, LC-MS
Objavljeno v RUNG: 14.01.2016; Ogledov: 7862; Prenosov: 303
Celotno besedilo (1,30 MB) |
