3481. Analysis of 19 Minerals and Cortisol in Red Deer Hair in Two Different Areas of the Stelvio National Park: A Preliminary StudyMarta Montillo, Tanja Peric, 2019, original scientific article Abstract: The aim of the study was to perform an investigation on the concentration of 19 minerals and
cortisol in red deer (Cervus elaphus) hair, a matrix that is easy to collect with non-invasive and painless
sampling, able to represent an integrative values of long-term substance concentrations, and able to
give useful information, also when performed on dead animals, given its extreme stability over time.
In the study thirty-five animals were included, coming from two different sides of a valley in the Stelvio
National Park, where official water analysis had pointed out elevated concentrations of As in one of the
two orographic sides. Hair cortisol concentrations were measured using a RIA(Radio Immuno Assay),
while minerals were detected using ICP-MS (Inductively Coupled Plasma- Mass Spectrometry).
Results showed a negative relationship between cortisol and some mineral concentrations (Li,
Co, As, Cd, Cr and Tl) and significant differences in some mineral concentrations between park
areas (Al, Co, Cu, Cd and Ni). As, Cr and cortisol differences approached statistical significance.
This preliminary study represents a step forward in the study of wildlife allostatic load and a valid
method for applications in wildlife management programs, in environmental studies and in public
health programs.
Keywords: hair, deer, mineral, cortisol
Published in RUNG: 27.08.2019; Views: 3015; Downloads: 97
 Full text (2,10 MB) |
3482. |
3483. |
3484. |
3485. Fibroblast involvement in soft connective tissue calcificationIvonne Pasquali Ronchetti, Federica Boraldi, Giulia Annovi, Paolo Cianciulli, Daniela Quaglino, 2013, review article Abstract: Soft connective tissue calcification is not a passive process, but the consequence of metabolic changes of local mesenchymal cells that, depending on both genetic and environmental factors, alter the balance between pro- and anti-calcifying pathways. While the role of smooth muscle cells and pericytes in ectopic calcifications has been widely investigated, the involvement of fibroblasts is still elusive. Fibroblasts isolated from the dermis of pseudoxanthoma elasticum (PXE) patients and of patients exhibiting PXE-like clinical and histopathological findings offer an attractive model to investigate the mechanisms leading to the precipitation of mineral deposits within elastic fibers and to explore the influence of the genetic background and of the extracellular environment on fibroblast-associated calcifications, thus improving the knowledge on the role of mesenchymal cells on pathologic mineralization.
Keywords: fibroblasts, PXE, PXE-likedisorders, elastin, extracellularmatrix, ectopiccalcification, mesenchymal
stromalcells
Published in RUNG: 23.08.2019; Views: 3192; Downloads: 0
This document has many files! More... |
3486. Matrix Gla Protein and Alkaline Phosphatase Are Differently Modulated in Human Dermal Fibroblasts from PXE Patients and ControlsFederica Boraldi, Giulia Annovi, Cees Vermeer, Leon J. Schurgers, Tommaso Trenti, Roberta Tiozzo, Deanna Guerra, Daniela Quaglino, 2013, original scientific article Keywords: Fibroblast, Vitamin K, Elastin calcification, Proteomics
Published in RUNG: 23.08.2019; Views: 3444; Downloads: 0
This document has many files! More... |
3487. Ectopic calcification in β-thalassemia patients is associated with increased oxidative stress and lower MGP carboxylationFederica Boraldi, Maria Immaculada Garcia Fernandez, Chiara Paolinelli DeVincenzi, Giulia Annovi, Leon J. Schurgers, Cees Vermeer, Paolo Cianciulli, Ivonne Pasquali Ronchetti, Daniela Quaglino, 2013, original scientific article Keywords: Elastin
Oxidative stress
Matrix Gla protein
Ectopic calcification
Beta-thalassemia
Published in RUNG: 23.08.2019; Views: 3236; Downloads: 0
This document has many files! More... |
3488. Heparan Sulfate Affects Elastin Deposition in Fibroblasts Cultured from Donors of Different AgesGiulia Annovi, Federica Boraldi, Pasquale Moscarelli, Deanna Guerra, Roberta Tiozzo, Bruna Parma, Pascal Sommer, Daniela Quaglino, 2012, original scientific article Abstract: Heparan sulfate (HS), due to its presence on the cell surface and in the extracellular milieu and its ability to
modulate cell signaling, has a fundamental role in both physiological and pathological conditions. For decades
we have demonstrated the occurrence of interactions between glycosaminoglycans (GAGs) and elastic fibers. In
particular, we have recently shown that HS is present inside elastic fibers and plays a role in the assembly and
stability of elastin coacervates. Elastin represents, within the extracellular matrix, the component most severely
affected during aging, and changes in the synthesis and posttranslational modifications of HS have been described,
possibly influencing cellular behavior and protein interactions. Thus, the present study has investigated,
in two different in vitro experimental models, the role of HS on elastin deposition and assembly. Results
demonstrate that: (1) Biological effects of HS are partly dependent on the physicochemical characteristics of the
GAGs; (2) HS does not affect attachment, viability, and growth of human dermal fibroblasts; (3) HS does not
modify elastin gene expression nor elastin synthesis, but favors a-elastin aggregation and, independently from
the age of donors, elastin assembly; (4) HS significantly increases the expression of fibulin 5, and these effects are
especially evident in fibroblasts isolated from aging donors. These data provide a better understanding of the
biological role of HS and offer new perspectives regarding the possibility of restoring and/or preserving the
elastic component with aging.
Keywords: Heparan sulfate, Elastin, Fibroblasts
Published in RUNG: 23.08.2019; Views: 3010; Downloads: 0
This document has many files! More... |
3489. Comparison of ex vivo and in vitro human fibroblast ageing models.Federica Boraldi, Giulia Annovi, Roberta Tiozzo, Pascal Sommer, Daniela Quaglino, 2010, original scientific article Abstract: Several studies have analyzed modulation of gene expression during physiological ageing with interesting, but often contradictory results, depending on the model used. In the present report we compare age-related metabolic and synthetic parameters in human dermal fibroblasts (HDF) isolated from young and old subjects (ex vivo ageing model) and cultured from early up to late cumulative population doublings (CPD) (in vitro ageing model) in order to distinguish changes induced in vivo by the aged environment and maintained in vitro, from those associated with cell senescence and progressive CPD. Results demonstrate that fibroblasts from aged donors, already at early CPD, exhibit an impaired redox balance, highlighting the importance of this parameter during ageing, even in the presence of standard environmental conditions, which are considered optimal for cell growth. By contrast, several proteins, as those related to heat shock response, or involved in endoplasmic reticulum and membrane trafficking, appeared differentially expressed only during in vitro ageing, suggesting that, at high CPD, the whole cell machinery becomes permanently altered. Finally, given the importance of the elastic component for a long-lasting connective tissue structural and functional compliance, this study focuses also on elastin and fibulin-5 synthesis and deposition, demonstrating a close relationship between fibulin-5 and ageing.
Keywords: Ageing
Fibroblast
Connective tissue
Oxidative stress
Protein expression
Elastin
Published in RUNG: 23.08.2019; Views: 3276; Downloads: 0
This document has many files! More... |
3490. |
