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2193. |
2194. Simultaneous ternary extension of DNA catalyzed by a trimeric replicase assembled in vivoAlejandro Montón Silva, Fabio Lapenta, Alessandra Stefan, Fabrizio Dal Piaz, Alessandro Ceccarelli, Alessandro Perrone, Alejandro Hochkoeppler, 2015, original scientific article Abstract: According to current models, dimeric DNA Polymerases coordinate the replication of DNA leading and lagging strands. However, it was recently shown that trimeric DNA Polymerases, assembled in vitro, replicate the lagging strand more efficiently than dimeric replicases. Here we show that the τ, α, ε, and θ subunits of Escherichia coli DNA Polymerase III can be assembled in vivo, yielding the trimeric τ3α3ε3θ3 complex. Further, we propose a molecular model of this complex, whose catalytic action was investigated using model DNA substrates. Our observations indicate that trimeric DNA replicases reduce the gap between leading and lagging strand synthesis.
Keywords: Trimeric DNA Polymerase, E. coli, DNA Polymerase III, τ3α3ε3θ3 complex, Triple DNA extension
Published in RUNG: 27.01.2021; Views: 2029; Downloads: 0
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2195. A pyrrolopyridazinedione-based copolymer for fullerene-free organic solar cellsAstrid-Caroline Knall, Samuel Rabensteiner, Sebastian F. Hoefler, Matiss Reinfelds, Mathias Hobisch, Heike M. A. Ehmann, Nadiia Pastukhova, Egon Pavlica, Gvido Bratina, Illie Hanzu, Shuguang Wen, Renqiang Yang, Gregor Trimmel, Thomas Rath, 2021, original scientific article Abstract: The recent success of non-fullerene acceptors in organic photovoltaics also entails a change in the requirements to the polymer donor in terms of optical and morphological properties leading to a demand for novel conjugated polymers. Herein, we report on the synthesis of a 1,4-bis-(thiophene-2-yl)-pyrrolopyridazinedione based copolymer with 2-ethylhexyl substituents on the pyrrolopyridazinedione moiety. A 2D conjugated benzodithiophene (BDT) was chosen as comonomer. The resulting copolymer T-EHPPD-T-EHBDT showed a molecular weight of 10.2 kDa, an optical band gap of 1.79 eV, a hole mobility of 1.8 × 10−4 cm2 V−1 s−1 and a preferred face-on orientation with regard to the substrate. The comparably wide band gap as well as the determined energy levels (HOMO: −5.47 eV, LUMO: −3.68 eV) match well with the narrow band gap non-fullerene acceptor ITIC-F, which was used as the acceptor phase in the bulk heterojunction absorber layers in the investigated solar cells. The solar cells, prepared in inverted architecture, revealed power conversion efficiencies up to 7.4% using a donor:acceptor ratio of 1 : 1 in the absorber layer.
Keywords: non-fullerene solar cells, charge transport, charge mobility, power conversion efficiency
Published in RUNG: 27.01.2021; Views: 2658; Downloads: 0
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2197. Effect of Humanizing Mutations on the Stability of the Llama Single-Domain Variable RegionMiguel Soler, Barbara Medagli, Jiewen Wang, Sandra Oloketuyi, Gregor Bajc, He Huang, Sara Fortuna, Ario De Marco, 2021, original scientific article Keywords: nanobody framework, modeling, nanobody humanization, CDR grafting
Published in RUNG: 27.01.2021; Views: 2430; Downloads: 65
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2198. Design, upgrade and characterization of the silicon photomultiplier front-end for the AMIGA detector at the Pierre Auger ObservatoryA. Aab, Danilo Zavrtanik, 2021, original scientific article Keywords: Observatorij Pierre Auger, kalibracija detektorjev, detektorji UV fotonov, diode PIN, Si-PMT, CCD
Published in RUNG: 26.01.2021; Views: 2341; Downloads: 74
Full text (17,36 MB) |
2199. Magnetic performance of SrFe12O19-Zn0.2Fe2.8O4 hybrid magnets prepared by Spark Plasma SinteringPetra Jenuš, Aleksander Učakar, S. Repše, Claudio Sangregorio, Mikkele Petrecca, M Albino, Cesar de Julian Fernandez, Blaž Belec, 2021, original scientific article Keywords: magnets, hybrid magnets, ferrites
Published in RUNG: 26.01.2021; Views: 2542; Downloads: 0
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2200. Real-time characterization and source apportionment of fine particulate matter in the Delhi megacity area during late winterVipul Lalchandan, Varun Kumar, Anna Tobler, M.T. Navaneeth, Suneeti Mishra, J. G. Slowik, Deepika Bhattu, Pragati Rai, Rangu Satish, Dilip Ganguly, Tiwari Tiwari, Neeraj Rastogi, Tiwari Sashi, Griša Močnik, André S. H. Prévôt, Sachchida Tripathi, 2021, original scientific article Abstract: National Capital Region (NCR) encompassing New Delhi is one of the most polluted urban metropolitan areas in the world.
Real-time chemical characterization of fine particulate matter (PM1 and PM2.5) was carried out using three aerosol mass
spectrometers, two aethalometers, and one single particle soot photometer (SP2) at two sites in Delhi (urban) and one site located
~40 km downwind of Delhi, during January-March, 2018. The campaign mean PM2.5 (NR-PM2.5 + BC) concentrations at the two
urban sites were 153.8±109.4 μg.m-3 and 127.8±83.2 μg.m-3, respectively, whereas PM1 (NR-PM1 + BC) was 72.3 ± 44.0 μg.m-3
at the downwind site. PM2.5 particles were composed mostly of organics (43-44)% followed by chloride (14-17)%, ammonium
(9-11)%, nitrate (9%), sulfate (8-10)%, and black carbon (11-16)%, whereas PM1 particles were composed of 47% organics,
13% sulfate as well as ammonium, 11% nitrate as well as chloride, and 5% black carbon. Organic aerosol (OA) source
apportionment was done using positive matrix factorization (PMF), solved using an advanced multi-linear engine (ME-2) model.
Highly mass-resolved OA mass spectra at one urban and downwind site were factorized into three primary organic aerosol
(POA) factors including one traffic-related and two solid-fuel combustion (SFC), and three oxidized OA (OOA) factors.
Whereas unit mass resolution OA at the other urban site was factorized into two POA factors related to traffic and SFC, and one
OOA factor. OOA constituted a majority of the total OA mass (45-55)% with maximum contribution during afternoon hours
~(70-80)%. Significant differences in the absolute OOA concentration between the two urban sites indicated the influence of local emissions on the oxidized OA formation. Similar PM chemical composition, diurnal and temporal variations at the three
sites suggest similar type of sources affecting the particulate pollution in Delhi and adjoining cities, but variability in mass concentration suggest more local influence than regional.
Keywords: source apportionment, air pollution, particulate matter, Delhi
Published in RUNG: 25.01.2021; Views: 3436; Downloads: 0
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