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3422. ДОСЛІДЖЕННЯ КІНЕТИКИ РЕАКЦІЙ РОЗПАДУ ФТАЛІМІД-N-ОКСИЛЬНИХ РАДИКАЛІВГанна Степаненко, Ольга Кущ, Катерина Новікова, Михайло Компанець, Йосип Опейда, 2016, published scientific conference contribution abstract Keywords: N-гідроксифталімід, фталімід-N-оксильні радикали, кінетика, розпад, константа швидкості, каталіз, УФ/vis-спектроскопія
Published in RUNG: 30.07.2019; Views: 1618; Downloads: 1
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3423. СПОНТАННИЙ РОЗПАД ФТАЛІМІД-N-ОКСИЛЬНИХ РАДИКАЛІВ РІЗНОЇ СТРУКТУРИГанна Степаненко, Олексій Андрєєв, Юрій Літвінов, Михайло Компанець, Ольга Кущ, Йосип Опейда, 2017, published scientific conference contribution abstract Keywords: N-гідроксифталімід, фталімід-N-оксильні радикали, кінетика, розпад, УФ/vis-спектроскопія
Published in RUNG: 30.07.2019; Views: 1447; Downloads: 0
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3427. Breathomics and its Application for Disease Diagnosis: A Review of Analytical Techniques and ApproachesDavid J Beale, Oliver A H Jones, Avinash V Karpe, Ding Y Oh, Iain R. White, Konstantinos A Kouremenos, Enzo A Palombo, 2018, independent scientific component part or a chapter in a monograph Abstract: The application of metabolomics to an ever-greater variety of sample types is a key focus of systems biology research. Recently, there has been a strong focus on applying these approaches toward the rapid analysis of metabolites found in non-invasively acquired samples, such as exhaled breath (also known as ‘breathomics’). The sampling process involved in collecting exhaled breath is nonintrusive and comparatively low-cost. It uses a series of globally approved methods and provides researchers with easy access to the metabolites secreted by the human body. Owing to its accuracy and rapid nature, metabolomic analysis of breath is a rapidly growing field that has proven effective in detecting and diagnosing the early stages of numerous diseases and infections. This review discusses the various collection and analysis methods currently applied in breathomics research. Some of the salient research completed in this field to date is also assessed and discussed in order to provide a basis for possible future scientific directions.
Keywords: Metabolomics, breath research, VOCs, breathomics
Published in RUNG: 22.07.2019; Views: 3182; Downloads: 0
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3428. Capturing and Storing Exhaled Breath for Offline AnalysisIain R. White, Stephen J Fowler, 2019, independent scientific component part or a chapter in a monograph Abstract: In this chapter we will summarize and discuss methods for the capture and storage of exhaled breath, prior to offline (and indirect online) analysis. We will detail and compare methods currently in use, including their applications, key strengths, and limitations. In synthesizing the best features of each technique, we will propose an ideal standardized breath sampling solution, and give a personal vision on the next steps to be taken in this exciting area of breath research.
Keywords: Breath analysis, Breath sampling, Offline analysis, Thermal desorption, Gas chromatography-mass spectrometry
Published in RUNG: 22.07.2019; Views: 3404; Downloads: 0
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3429. The effect of serum withdrawal on the protein profile of quiescent human dermal fibroblasts in primary cell culture.Boraldi Federica, Annovi Giulia, Paolinelli Devincenzi Chiara, Tiozzo Roberta, Quaglino Daniela, 2008, original scientific article Abstract: The effect of serum deprivation on proliferating cells is well known, in contrast its role on primary
cell cultures, at confluence, has not been deeply investigated. Therefore, in order to explore
the response of quiescent cells to serum deprivation, ubiquitous mesenchymal cells, as normal
human dermal fibroblasts, were grown, for 48 h after confluence, in the presence or absence of
10% FBS. Fibroblast behaviour (i.e. cell morphology, cell viability, ROS production and elastin
synthesis) was evaluated morphologically and biochemically. Moreover, the protein profile was
investigated by 2-DE and differentially expressed proteins were identified by MS. Serum withdrawal
caused cell shrinkage but did not significantly modify the total cell number. ROS production,
as evaluated by the dihydroethidium (DH2) probe, was increased after serum deprivation,
whereas elastin synthesis, measured by a colorimetric method, was markedly reduced in the
absence of serum. By proteome analysis, 41 proteins appeared to significantly change their
expression, the great majority of protein changes were related to the cytoskeleton, the stress response
and the glycolytic pathway. Data indicate that human dermal fibroblasts in primary cell
culture can adapt themselves to environmental changes, without significantly altering cell viability,
at least after a few days of treatment, even though serum withdrawal represents a stress
condition capable to increase ROS production, to influence cell metabolism and to interfere with
cell behaviour, favouring the expression of several age-related features.
Keywords: Dermal fibroblasts / Primary cell culture / ROS production / Serum withdrawal
Published in RUNG: 22.07.2019; Views: 3347; Downloads: 0
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3430. Hypoxia influences the cellular cross-talk of human dermal fibroblasts. A proteomic approach.Boraldi Federica, Annovi Giulia, Carraro Fabio, Naldini Antonella, Tiozzo Roberta, Sommer Pascal, Quaglino Daniela, 2007, original scientific article Abstract: The ability of cells to respond to changes in oxygen availability is critical for many physiological and pathological processes (i.e. development,
aging, wound healing, hypertension, cancer). Changes in the protein profile of normal human dermal fibroblasts were investigated in vitro after
96 h in 5% CO2 and 21% O2 (pO2=140 mm Hg) or 2% O2 (pO2=14 mm Hg), these parameters representing a mild chronic hypoxic exposure
which fibroblasts may undergo in vivo. The proliferation rate and the protein content were not significantly modified by hypoxia, whereas
proteome analysis demonstrated changes in the expression of 56 proteins. Protein identification was performed by mass spectrometry. Data
demonstrate that human fibroblasts respond to mild hypoxia increasing the expression of hypoxia inducible factor (HIF1a) and of the 150-kDa
oxygen-regulated protein. Other differentially expressed proteins appeared to be related to stress response, transcriptional control, metabolism,
cytoskeleton, matrix remodelling and angiogenesis. Furthermore, some of them, like galectin 1, 40S ribosomal protein SA, N-myc-downstream
regulated gene-1 protein, that have been described in the literature as possible cancer markers, significantly changed their expression also in
normal hypoxic fibroblasts. Interestingly, a bovine fetuin was also identified that appeared significantly less internalised by hypoxic fibroblasts. In
conclusion, results indicate that human dermal fibroblasts respond to an in vitro mild chronic hypoxic exposure by modifying a number of
multifunctional proteins. Furthermore, data highlight the importance of stromal cells in modulating the intercellular cross-talk occurring in
physiological and in pathologic conditions.
Keywords: Human fibroblast, Primary cell culture, Hypoxia, Connective tissue, Proteome, 2D gel electrophoresis, Mass-spectrometry
Published in RUNG: 22.07.2019; Views: 3402; Downloads: 0
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