5331. Application of high-performance liquid chromatography combined with ultra-sensitive thermal lens spectrometric detection for simultaneous biliverdin and bilirubin assessment at trace levels in human serumMitja Martelanc, Lovro Žiberna, Sabina Passamonti, Mladen Franko, 2016, original scientific article Abstract: We present the applicability of a new ultra sensitive analytical method for the simultaneous determination of biliverdin and bilirubin in human serum. The method comprises isocratic reversed-phase(RP) C18 high-performance liquid chromatography (HPLC) and thermal lens spectrometric detection (TLS)based on excitation by a krypton laser emission line at 407 nm. This method enables the separation of IX-α biliverdin and IX-α bilirubin in 11 min. with limit of detection (LOD) and limit of quantitation (LOQ) for
biliverdin of 1.2 nM and 3 nM, and 1nM and 2.8 nM for bilirubin,respectively.In addition, a step-gradient elution was set up, by changing the mobile phase composition, in order to further enhance the sensitivity for bilirubin determination with LOD and LOQ of 0.5 nM and 1.5 nM, respectively. In parallel, an isocraticHPLC-DAD method was developed for benchmarking against HPLC-TLS methods. The LOD
and LOQ forbiliverdin were 6 nM and 18 nM, and 2.5 nM and 8nM for bilirubin,respectively. Additionally, both isocratic methods were applied for measuring biliverdin and free bilirubin in human serum samples (from 2 male and 2 female healthy donors). Combining isocratic HPLC method with TLS
detector was crucial for first ever biliverdin determination in serum together with simultaneous free bilirubin determination. We showed for the first time the concentration ratio of free bilirubin versus unbound biliverdin in human serum samples.
Keywords: Bilirubin, biliverdin, serum, HPLC, TLS
Published in RUNG: 29.03.2016; Views: 5648; Downloads: 0
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5333. FlashCam: a fully-digital camera for the medium-sized telescopes of the Cherenkov Telescope ArrayG. Pühlhofer, Andrej Filipčič, Gašper Kukec Mezek, Samo Stanič, Serguei Vorobiov, Lili Yang, Gabrijela Zaharijas, Danilo Zavrtanik, Marko Zavrtanik, 2015, published scientific conference contribution Keywords: Cherenkov Telescope Array (CTA), medium-sized telescopes (MST), imaging camera, FlashCam fully-digital readout system
Published in RUNG: 24.03.2016; Views: 4525; Downloads: 195
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5334. NectarCAM : a camera for the medium size telescopes of the Cherenkov Telescope ArrayJ-F. Glicenstein, Andrej Filipčič, Gašper Kukec Mezek, Samo Stanič, Serguei Vorobiov, Lili Yang, Gabrijela Zaharijas, Danilo Zavrtanik, Marko Zavrtanik, 2015, published scientific conference contribution Keywords: Cherenkov Telescope Array (CTA), medium-sized telescopes (MST), imaging camera, NectarCAM design, NECTAr ASIC, Switched Capacitor Array
Published in RUNG: 24.03.2016; Views: 4680; Downloads: 237
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5335. Development of the photomultiplier tube readout system for the first Large-Sized Telescope of the Cherenkov Telescope ArrayShu Masuda, Andrej Filipčič, Gašper Kukec Mezek, Samo Stanič, Serguei Vorobiov, Lili Yang, Gabrijela Zaharijas, Danilo Zavrtanik, Marko Zavrtanik, 2015, published scientific conference contribution Keywords: Cherenkov Telescope Array (CTA), Large-Sized Telescope (LST), imaging camera, readout system
Published in RUNG: 24.03.2016; Views: 4670; Downloads: 310
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5336. Na Kanalskem velike možnosti za pridelavo kakovostnega sadjaZoran Božič, 2016, interview Abstract: Dr. Zoran Božič je profesor na SETŠ Nova Gorica in predavatelj na Univerzi v Novi Gorici, v zadnjem mandatu je bil izvoljen v državni zbor kot predstavnik vzgoje in izobraževanja. V prostem času, ki ga sicer ni ravno veliko, se ukvarja z vzgojo starih sort sadnega drevja.
Keywords: sadjarstvo, stare sadne sorte, Goriška, sadovnjak Pod skalco, ekologija
Published in RUNG: 24.03.2016; Views: 5420; Downloads: 238
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5337. Asymmetric WIMP Dark Matter in the presence of DM/anti-DM OscillationsGabrijela Zaharijas, 2012, published scientific conference contribution abstract Abstract: The class of ‘Asymmetric Dark Matter’ scenarios relies on the existence of a primordial
particle/anti-particle asymmetry in the dark sector related to the baryon asymmetry as a
way to address the observed similarity between the baryonic and dark matter energy densities
today. Focusing on this framework we calculate the evolution of the dark matter relic
abundance in the presence of particle/anti-particle oscillations. We show how oscillations reopen
the parameter space of asymmetric dark matter models, in particular in the direction of
allowing large (WIMP-scale) DM masses. Finally, we constrain the parameter space in this
framework by applying up-to-date bounds from indirect detection signals on annihilating DM.
Keywords: dark matter, early universe
Published in RUNG: 23.03.2016; Views: 4641; Downloads: 0
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5338. Empirical Research: How to Improve Reception of Classical PoetryZoran Božič, 2015, original scientific article Abstract: An empirical study of understanding The Baptism at the Savica showed that Slovenian high school students had notable difficulties in decoding the basic meanings of Prešeren’s poem. In literature, didactics offer three methods of facilitating
reception of complex classical poetry: linguistic modernisation, addition of notes,and transmission into prose. Due to the cult status of Prešeren’s poetry, modernizations can only be limited, while commenting hinders a spontaneous reading experience;
as a result, only prosification entirely solves the problems of pronounced archaization, inverted word order and abundant metaphors in Prešeren’s lines.This was confirmed by an empirical study in which high school students, who read the prozification together with the verses from The Baptism at the Savica, attested
a comprehension which was twice as good as comprehension of students who only read the poetry.
Keywords: empirical research, reception, France Prešeren, The Baptism at the
Savica, prosification
Published in RUNG: 23.03.2016; Views: 4928; Downloads: 226
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5339. Development of Slow Control Boards for the Large Size Telescopes of the Cherenkov Telescope ArrayDaniela Hadasch, Andrej Filipčič, Gašper Kukec Mezek, Samo Stanič, Serguei Vorobiov, Lili Yang, Gabrijela Zaharijas, Danilo Zavrtanik, Marko Zavrtanik, published scientific conference contribution Keywords: Cherenkov Telescope Array (CTA), Large Size Telescopes (LST), imaging camera, slow control board (SCB)
Published in RUNG: 21.03.2016; Views: 4408; Downloads: 197
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5340. The Optical System for the Large Size Telescope of the Cherenkov Telescope ArrayM. Hayashida, Andrej Filipčič, Gašper Kukec Mezek, Samo Stanič, Serguei Vorobiov, Lili Yang, Gabrijela Zaharijas, Danilo Zavrtanik, Marko Zavrtanik, published scientific conference contribution Keywords: Cherenkov Telescope Array (CTA), Large Size Telescopes (LST), optical system, active mirror control system
Published in RUNG: 21.03.2016; Views: 4550; Downloads: 224
Full text (1,10 MB) |
