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5413. Reducing treatments in cattle superovulation protocols by combining a pituitary extract with a 5% hyaluronan solution: Is it able to diminish activation of the hypothalamic pituitary adrenal axis compared to the traditional protocol?Andrea Biancucci, Tatiana Sbaragli, Antonella Comin, Lakamy Sylla, Maurizio Monaci, Tanja Peric, Giuseppe Stradaioli, 2016, izvirni znanstveni članek Opis: Traditional superovulation protocols that include multiple gonadotropin treatments are time-consuming and labor intensive. These protocols require multiple handling and restraining of embryo donors. This will likely increase the risks of injuries in both animals and humans and induce stress that may lead to a reduced superovulatory response. These are more evident when working with cattle that are rarely handled or raised on extensive grazing. The objectives of this experiment were to compare the efficacy of a split-injection protocol of porcine pituitary-derived porcine FSH (pFSH) preparation (slow release [SR] group) to the traditional 4-day treatment with pFSH administered twice daily (C group) and to determine the concentrations of cortisol in the hair as a marker of activation of the hypothalamic-pituitary-adrenal (HPA) axis during the two superovulatory treatments. Thirty-two heifers were stimulated twice in a 2 × 2 crossover design and compared for ovarian response and numbers and characteristics of recovered ova-embryo among treatments. No differences between SR and C groups were found in terms of percentage of responsive animals (100% vs. 93.8%) and ovulation rate (83.7 ± 1.1 vs. 79.5 ± 1.0%). A positive correlation was found between the number of follicles responsive to pFSH (2-8 mm) at the beginning of treatments and the superovulatory response, and no differences were found in these follicular populations between the two treatment groups. The numbers of CLs, ova-embryos, fertilized ova, transferable and freezable embryos recovered per cow were found to be significantly higher in SR compared with C group (14.0 ± 1.6 vs. 10.6 ± 1.0, 12.1 ± 1.6 vs. 7.6 ± 1.0, 11.1 ± 1.1 vs. 7.3 ± 1.0, 9.6 ± 1.4 vs. 6.6 ± 1.0, and 9.4 ± 1.4 vs. 6.0 ± 1.0 for SR and C group, respectively). The SR group produced also a significantly greater number of excellent- and/or good-quality embryos compared with the C group. The concentrations of cortisol in the hair at Days 14 and 21 were significantly greater in the C compared with the SR group (P < 0.05). In conclusion, these results indicate that the dilution of gonadotropin in a 5% hyaluronan solution, reducing the administration frequency, improves the quantitative and qualitative superovulatory response of Marchigiana heifers. Further studies using other breeds of cattle are needed to verify the results herein obtained and to confirm the lower activation of the hypothalamic-pituitary-adrenal axis caused in the donor by the split-injection protocol.
Ključne besede: Beef heifer, Bovine, Hair cortisol, Hyaluronic acid, Slow release, Superovulation
Objavljeno v RUNG: 01.03.2016; Ogledov: 4609; Prenosov: 312
 Celotno besedilo (403,15 KB)
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5414. Prototype muon detectors for the AMIGA component of the Pierre Auger ObservatoryA. Aab, Andrej Filipčič, Gašper Kukec Mezek, Ahmed Saleh, Samo Stanič, Marta Trini, Serguei Vorobiov, Lili Yang, Danilo Zavrtanik, Marko Zavrtanik, 2015, izvirni znanstveni članek Ključne besede: detektor mionov, detektor Čerenkova, AMIGA, P. Auger Observatory
Objavljeno v RUNG: 29.02.2016; Ogledov: 5419; Prenosov: 0
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5416. Control of the Polarization of a Vacuum-Ultraviolet, High-Gain, Free-Electron LaserEnrico Allaria, Giovanni De Ninno, Primož Rebernik Ribič, David Gauthier, C. Grazioli, Barbara Ressel, 2014, izvirni znanstveni članek Ključne besede: laser na proste elektrone, relativistični elektroni, polarizacija
Objavljeno v RUNG: 29.02.2016; Ogledov: 5014; Prenosov: 443
Celotno besedilo (1,92 MB) |
5417. Experimental Demonstration of Electron Longitudinal-Phase-Space Linearization by Shaping the Photoinjector Laser PulseG. Penco, M. Danailov, A. Demidovich, Enrico Allaria, Giovanni De Ninno, S. Di Mitri, W. Fawley, E. Ferrari, L. Giannessi, M. Trovó, 2014, izvirni znanstveni članek Ključne besede: laser na proste elektrone, linearizacija, relativistični elektroni
Objavljeno v RUNG: 29.02.2016; Ogledov: 4584; Prenosov: 0
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5420. 2FHL : THE SECOND CATALOG OF HARD FERMI-LAT SOURCESM. Ackermann, Gabrijela Zaharijas, Collaboration Fermi LAT, 2016, izvirni znanstveni članek Opis: We present a catalog of sources detected above 50 GeV by the Fermi-Large Area Telescope (LAT) in 80 months of data. The newly delivered Pass8 event-level analysis allows the detection and characterization of sources in the 50 GeV–2 TeV energy range. In this energy band, Fermi-LAT has detected 360 sources, which constitute the second catalog of hard Fermi-LAT sources (2FHL). The improved angular resolution enables the precise localization of point sources (∼1 7 radius at 68% C.L.) and the detection and characterization of spatially extended sources. We find that 86% of the sources can be associated with counterparts at other wavelengths, of which the majority (75%) are active galactic nuclei and the rest (11%) are Galactic sources. Only 25% of the 2FHL sources have been previously detected by Cherenkov telescopes, implying that the 2FHL provides a reservoir of candidates to be followed up at very high energies. This work closes the energy gap between the observations performed at GeV energies by Fermi-LATon orbit and the observations performed at higher energies by Cherenkov telescopes from the ground.
Ključne besede: catalogs – gamma-rays
Objavljeno v RUNG: 25.02.2016; Ogledov: 5258; Prenosov: 0
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