11. Cider yeasts associated with Hardanger cider during fermentation processUrban Česnik, Mitja Martelanc, Branka Mozetič Vodopivec, Ingunn Ovsthus, Lorena Butinar, 2022, published scientific conference contribution abstract Abstract: In the Hardanger area in Western Norway, the production of cider has a long tradition that goes back to the 12th century, when monks introduced apple growing in this area. Nowadays, this is also the main area of fruit production in Norway. Despite the strict regulation of the alcoholic beverage production in Norway, traditional cider is still produced on some farms in this area. Therefore, our aim was to study the ecology and biodiversity of the yeasts associated with the cider production in the Hardanger area during fermentation process; especially of traditional cider, which is produced by a spontaneous fermentation of apple juice, performed by naturally occurring indigenous yeasts that originate from the fruit or the surfaces of the processing equipment.
In our study, samples of fermenting juice/cider were taken during fermentation process from 12 producers, located in 12 different locations in Hardanger region. Classical cultivation methods using WL (Wallerstein Laboratories) agar medium with added chloramphenicol enable us to isolate a total of 530 yeast isolates that were stored in in-house yeast collection at the NIBIO and included also at the Wine Research Centre collection. Based on the sequencing of the D1/D2 domain of the 26S rDNA we managed to identify 357 isolates and distinguished 27 different yeast species as follows: Aureobasidium pullulans, Candida californica, C. oleophila, C, sake, Hanseniaspora meyeri, H. uvarum, H. valbyensis. Kregervanrija fluxuum, Kregervanrija sp., Metschnikowia andauensis, M. chrysoperlae, M. fructicola, M. pulcherrima, Metschnikowia sp, Pichia fermentans, P. kluyveri, P. membranifaciens, P. nakasei, Piskurozyma capsuligena, Rhodotorula nothofagi, Saccharomyces bayanus, S. cerevisiae, S. paradoxus, S. pastorianus, Saccharomyces sp., S. uvarum and Torulaspora delbrueckii.
Even though we were not able to obtain samples in three different fermentation stages (beginning, middle and at the end of fermentation) from all producers, we could observe yeast succession during fermentation progress. Yeast diversity was higher at the beginning comparing to the middle of fermentation, when mostly different non-Saccharomyces yeast species prevailed, while in the middle of fermentation 11 species were detected (Candida californica, H. uvarum, H. valbyensis, Kregervanrija sp., K. fluxuum, Pichia membranifaciens, Metschnikowia pulcherrima, Saccharomyces sp, S. bayanus, S. uvarum and S. cerevisie). On the other hand, at the end of fermentation mainly Saccharomyces species with high ethanol tolerance were present (Saccharomyces sp., S. cerevisiae, bayanus, S. uvarum and P. fermentans).
In samples that were collected from three producers in all three fermentation stages also quality parameters were determined (ethanol, organic acids, sugars, biogenic amines) with in-house developed methods using HPLC-UV/RID. The most important sugars in ciders were fructose and glucose, as expected. Two producers added sugar to increase the level of ethanol in the middle of fermentation, which is a common procedure in the Hardanger area. Ethanol and organic acid analysis indicated that fermentations went in the right direction, since all parameters were within normal limits. Including the acetic acid level, an indicator of low cider quality, was very low (average around 0,06 g/L). The alcohol incised from the beginning to end fermentation in all samples analysed and minimum concentration was 2,71 g/L. In ciders we detected four biogenic amines (putrescin,
cadaverine, histamine and tyramine). The average amount was 32 mg/L and the most abundant was tyramine. Keywords: indigenous yeasts, biodiversity, spontaneous fermentation, cider-making Published in RUNG: 18.10.2022; Views: 1288; Downloads: 0 This document has many files! More... |
12. Gaia Early Data Release 3 : Gaia photometric science alertsSimon Hodgkin, D. L. Harrison, E. Breedt, T. Wevers, Guy Rixon, A. Delgado, A. Yoldas, Z. Kostrzewa-Rutkowska, Mitja Fridman, Andreja Gomboc, 2021, original scientific article Keywords: Gaia satellite, transients, alerts Published in RUNG: 02.11.2021; Views: 1715; Downloads: 8 Link to full text This document has many files! More... |
13. The influence of selected indigenous yeasts on Pinot Noir wine colour propertiesJelena Topić, Lorena Butinar, Guillaume Antalick, Melita Sternad Lemut, Mitja Martelanc, Alen Albreht, Dorota Korte, Branka Mozetič Vodopivec, 2021, original scientific article Keywords: Pinot Noir, wine colour, yeasts, non-Saccharomyces, sensory Published in RUNG: 07.07.2021; Views: 2198; Downloads: 0 This document has many files! More... |
14. Laser ablation-single-particle-inductively coupled plasma mass spectrometry as a multimodality bioimaging tool in nano-based omicsDino Metarapi, Johannes Teun van Elteren, Martin Šala, Katarina Vogel-Mikuš, Iztok Arčon, Vid Simon Šelih, Mitja Kolar, Samo B. Hočevar, 2021, original scientific article Abstract: Advanced data processing and visualization techniques were applied to laser ablation-single particleinductively
coupled plasma mass spectrometry (LA-sp-ICPMS) data obtained from imaging of roots of
sunflower (Helianthus annuus L.) which were exposed to ionic silver (Ag+). Highly multiplexed images with
tissue level resolution were generated, showing the uptake and transformation of Ag+ to silver
nanoparticles (AgNPs) in whole root cross-sections, and revealing that the size of biosynthesized AgNPs is
predisposed by the reducing power of particular root compartments. Various Ag+ and AgNP visualization
strategies are shown with special emphasis on spatially resolved information associated with the number
and spherical-equivalent size of individual AgNPs in user-selected root cross-sectional regions, including
statistical analysis of the AgNP distribution. Normally, this multimodality information can only be obtained
by combining the results of techniques from several complementary platforms. Keywords: Laser ablation, Ag XANES, bioimaging Published in RUNG: 03.06.2021; Views: 2139; Downloads: 50 Link to full text This document has many files! More... |
15. Preparation of porous [alpha]-Fe[sub]2O[sub]3 thin films for efficient photoelectrocatalytic degradation of basic blue 41 dyeManel Machreki, Takwa Chouki, Mitja Martelanc, Lorena Butinar, Branka Mozetič Vodopivec, Saim Emin, 2021, original scientific article Abstract: A novel method was developed for the preparation of porous hematite (α-Fe2O3) thin films. First, a solution containing iron precursor was spin-coated on fluorine-doped tin oxide substrate and later short heat-treated at 750 °C. The prepared α-Fe2O3 thin films were applied as dual-function catalyst in photoelectrochemical (PEC) water oxidation and textile dye degradation studies. For the first time, α-Fe2O3 thin films were used in efficient PEC degradation of a textile dye (Basic Blue 41 – B41) using in-situ generated reactive chlorine species. In comparison with photocatalytic and electrocatalytic approaches, the PEC technique allows faster degradation of B41 dye at an applied bias potential of 1.5 V versus reversible hydrogen electrode and under visible light illumination. In the presence of Cl− using the PEC approach the degradation of B41 reaches 99.8%. High-performance liquid chromatography coupled with UV–VIS system confirmed the degradation of B41 dye using PEC. Gas-chromatography coupled to mass spectrometry was used to study the by-products obtained during PEC degradation. Chemical oxygen demand analyses confirmed that the mineralization level of B41 is in the order of 68%. The α-Fe2O3 films developed in this study give a higher level of PEC degradation efficiency compared to other iron oxide-based systems. Keywords: thin films, photoelectrocatalysis, kinetics, visible light, degradation, textile dye Published in RUNG: 10.05.2021; Views: 2430; Downloads: 12 Link to full text This document has many files! More... |
16. Temporal and spatial patterns of zinc and iron accumulation during barley (Hordeum vulgare L.) grain development. Journal of agricultural and food chemistry.Amelie Detterbeck, Paula Pongrac, Daniel Persson, Katarina Vogel-Mikuš, Mitja Kelemen, Primož Vaupetič, Primož Pelicon, Iztok Arčon, Søren Husted, Jan Kofod Shjoerring, Stephan Clemens, 2020, original scientific article Abstract: Breeding and engineering of biofortified crops will benefit from a better understanding of bottlenecks controlling
micronutrient loading within the seeds. However, few studies have addressed the changes in micronutrient concentrations,
localization, and speciation occurring over time. Therefore, we studied spatial patterns of zinc and iron accumulation during grain
development in two barley lines with contrasting grain zinc concentrations. Microparticle-induced-X-ray emission and laser ablationinductively
coupled plasma mass spectrometry were used to determine tissue-specific accumulation of zinc, iron, phosphorus, and
sulfur. Differences in zinc accumulation between the lines were most evident in the endosperm and aleurone. A gradual decrease in
zinc concentrations from the aleurone to the underlying endosperm was observed, while iron and phosphorus concentrations
decreased sharply. Iron co-localized with phosphorus in the aleurone, whereas zinc co-localized with sulfur in the sub-aleurone. We
hypothesize that differences in grain zinc are largely explained by the endosperm storage capacity. Engineering attempts should be
targeted accordingly. Keywords: barley (Hordeum vulgare L.), biofortification, grain development, grain loading, LA-ICP-MS, μ-PIXE Published in RUNG: 20.10.2020; Views: 2812; Downloads: 0 This document has many files! More... |
17. Povečanje izkoristka sončne elektrarne z uporabo optimizatorjev močiMitja Brumen, 2020, undergraduate thesis Abstract: Diplomsko delo je bilo pripravljeno z namenom ugotoviti smotrnost investicije v sončno elektrarno z vgradnjo razsmernikov z optimizatorji moči.
Diplomsko delo predstavi konkreten primer sončne elektrarne, katero namerava investitor postaviti na strehi garaže, ki stoji ob stanovanjski hiši. Streha stanovanjske hiše tako ob določenih delih dneva senči površino, na katero bodo nameščeni fotonapetostni moduli, dodatno pa bodo osenčeni tudi zaradi drevesa, ki stoji ob stavbi. S pomočjo programa PV*SOL premium smo preverili predvideno letno sončno obsevanje na dotični lokaciji, dobljene podatke pa upoštevali kot podlago pri izračunu predvidene proizvodnje električne energije. Primerjali smo predvideno količino proizvodnje z vgradnjo razsmernika brez optimizatorjev moči ter z optimizatorji moči. Dodatno smo opravili ekonomski izračun investicije obeh primerov ter po primerjavi ugotovili, da je investicija v sončno elektrarno z vgradnjo razsmernika z optimizatorji moči kljub začetni višji investiciji smiselna. Keywords: Fotovoltaika, sončna elektrarna, sončno sevanje, osenčenost, razsmernik, optimizator moči Published in RUNG: 30.06.2020; Views: 4930; Downloads: 195 Full text (2,72 MB) |
18. Recent advances in 2D imaging of element distribution in Plants by focused beam techniquesKatarina Vogel-Mikuš, Johannes Teun van Elteren, Marjana Regvar, Jitrin Chaiprapa, Boštjan Jenčič, Iztok Arčon, Alojz Kodre, Peter Kump, Anja Kavčič, Mitja Kelemen, Dino Metarapi, Marijan Nečemer, Primož Vavpetič, Primož Pelicon, Paula Pongrac, 2019, independent scientific component part or a chapter in a monograph Keywords: 2D Imiging, XRF, XANES, synchrotron light Published in RUNG: 05.09.2019; Views: 3297; Downloads: 0 This document has many files! More... |
19. Efficient traffic regulation based on urban Black Carbon measurements and prediction modelAsta Gregorič, Luka Drinovec, Griša Močnik, Anja Barle, Matija Marolt, Jernej Henigman, Borut Šuštar, Mitja Ferlan, Andrej Pangeršič, 2018, published scientific conference contribution abstract Keywords: air quality, black carbon Published in RUNG: 29.10.2018; Views: 3684; Downloads: 0 This document has many files! More... |
20. Cadmium localization and chemical environment in a cadmium bioindicator Gomphrena clausseniiPaula Pongrac, Mina T. Villafort Carvalho, Tânia S. Serra, Hiram Castillo Michel, Katarina Vogel-Mikuš, Iztok Arčon, Luka Jeromel, Mitja Kelemen, Boštjan Jenčič, Anja Kavčič, Primož Pelicon, Mark G. M. Aarts, 2018, published scientific conference contribution abstract Keywords: cadmium Published in RUNG: 12.09.2018; Views: 3657; Downloads: 0 This document has many files! More... |