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21.
Regulation and controlling the motility properties of Pseudomonas aeruginosa
Fazlurrahman Khan, Dung Thuy Nguyen Pham, Sandra Oloketuyi, Young-Mog Kim, 2020, pregledni znanstveni članek

Opis: Chronic infections caused by Pseudomonas aeruginosa have been a major concern as their spread and mortality continue to be on the rise. These infections are majorly attributed to biofilm formation via sequential steps where motility plays an essential role in initial attachment of bacterial cells onto biotic and abiotic surfaces, thereby contributing to multi-drug resistance among pathogens. Therefore, attenuating motility properties can be considered as highly potential for controlling P. aeruginosa biofilm formation. This strategy has employed the use of various natural and chemically synthesized compounds. The present review article explained the importance and regulation of different types of motilities properties. Furthermore, it also covered several important alternative approaches using anti-motility agents which could be helpful for controlling P. aeruginosa biofilm-associated infections. Further studies are required for in-depth understandings about the mechanisms of motilities controlling of these molecules at molecular levels.
Ključne besede: Biofilm, Motility, Attenuation, Antibiofilm drugs, Pseudomonas aeruginosa
Objavljeno v RUNG: 12.01.2021; Ogledov: 2549; Prenosov: 0
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22.
FUNCTIONALIZED NANOBODIES AS DIAGNOSTIC REAGENTS FOR THE DETECTION OF TOXIC MICROALGAE
Sandra Oloketuyi, 2020, doktorska disertacija

Opis: Early detection and monitoring of toxic microalgae remains a challenge that will be necessary to overcome for understanding harmful algal bloom events and their consequent ecological and environmental impacts. Although there are diagnostic techniques based on microscopy, fluorescence in situ hybridization (FISH), DNA microarrays, real-time PCR, sandwich hybridization assays employed for the detection of harmful algal species, such techniques are not suitable for field monitoring and especially for the quantification of Alexandrium minutum due to abundance of cryptic species that cannot be differentiated based on morphology or genomic traits. These approaches are also time-consuming and laborious, hence there is need for alternative simple, rapid and cost-effective methods. In this study, our aim was to develop different nanobody-based capture strategies for the detection of A. minutum. Nanobodies were successfully displayed on the surface of Escherichia coli to facilitate detection of A. minutum. The method is simple and cost effective as no antibody purification step is necessary. As an alternative, A. minutum was quantified by exploiting the peroxidase activity of a G-quadruplex DNAzyme covalently bound to nanobodies and by means of a label free electrochemical immunosensor which exploit nanobodies bound via an irreversible SpyTag-SpyCatcher system as the specific immunocapture element. Such immunosensor was prepared by immobilizing the SpyTagged anti-A. minutum nanobody (SpyTagged C1) by means of a selfassembled monolayer (SAMs) of L- cysteine (L-Cys) displayed onto a gold nanoparticle-modified glassy carbon electrode. Electrochemical Impedance Spectroscopy (EIS) was performed to measure the electrical response of the circuit as the function of A. minutum immunocaptured cells. The results of EIS studies confirmed that the sensing chip detected A. minutum selectively, exhibited a wide dynamic range spanning from 103 to 109 cell L-1 and a limit of detection of 3.1× 103 cell L-1. The immunosensor data can be recorded and then analyzed with a portable potentiostat. Such device can be interfaced with a smart phone, a condition suitable for the rapid A. minutum quantification in situ.
Ključne besede: Alexandrium minutum, nanobodies, bioreagents, immunosensor, toxic microalgae
Objavljeno v RUNG: 17.06.2020; Ogledov: 3982; Prenosov: 231
.pdf Celotno besedilo (2,95 MB)

23.
Peroxidase zymograms obtained by agarose native gel electrophoresis have unmet resolution and completeness
Sandra Oloketuyi, Giulia Annovi, Ario De Marco, 2020, izvirni znanstveni članek

Ključne besede: zymograms, peroxidase, agarose gel, forest tree, biochemical biomarkers
Objavljeno v RUNG: 22.04.2020; Ogledov: 2820; Prenosov: 0
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Bacterial surface display of nanobodies against cancer and toxic micoalgal cells
Sandra Oloketuyi, Carina Dilkaute, Elisa Mazzega, Joachim Jose, Ario De Marco, 2019, objavljeni povzetek znanstvenega prispevka na konferenci

Ključne besede: bacteria surface display, nanobodies, HER2, A. minutum
Objavljeno v RUNG: 18.11.2019; Ogledov: 3269; Prenosov: 99
.pdf Celotno besedilo (12,18 KB)

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A compact nanobody-DNAzyme conjugate enables antigen detection and signal amplification
Giulio Bernardinelli, Sandra Oloketuyi, Franziska Werner, Elisa Mazzega, Bjorn Hogberg, Ario De Marco, 2020, izvirni znanstveni članek

Ključne besede: DNAzyme, nanobodies, diagnostics, A. minutum
Objavljeno v RUNG: 10.11.2019; Ogledov: 3248; Prenosov: 0
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Purification-independent immunoreagents obtained by displaying nanobodies on bacteria surface
Sandra Oloketuyi, Carina Dilkaute, Elisa Mazzega, Joachim Jose, Ario De Marco, 2019, izvirni znanstveni članek

Ključne besede: Bacterial display, immunocapture, diagnostics, nanobodies
Objavljeno v RUNG: 18.04.2019; Ogledov: 2828; Prenosov: 0
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