1. Biological applications of synthetic binders isolated from a conceptually new adhiron libraryClaudia D'Ercole, Matteo De March, Gianluca Veggiani, Sandra Oloketuyi, Rossella Svigelj, Ario De Marco, 2023, izvirni znanstveni članek Ključne besede: synthetic library, adhirons, affimers, C-reactive protein, SpyCatcher Objavljeno v RUNG: 17.10.2023; Ogledov: 643; Prenosov: 3 Celotno besedilo (2,23 MB) Gradivo ima več datotek! Več... |
2. Covalent organic frameworks for fluorescent imaging of hypoxiaTina Škorjanc, Dinesh Shetty, Gregor Mali, Damjan Makuc, Martina Bergant Marušič, Matjaž Valant, 2023, objavljeni povzetek znanstvenega prispevka na konferenci Ključne besede: hypoxia, covalent organic frameworks, imaging, fluorescence, post-synthetic modification Objavljeno v RUNG: 06.06.2023; Ogledov: 944; Prenosov: 2 Povezava na datoteko Gradivo ima več datotek! Več... |
3. Structural basis of human clamp sliding on DNAMatteo De March, N. Merino, S. Barrera-Vilarmau, R. Crehuet, S. Onesti, Francisco J. Blanco-Silva, A. De Biasio, 2018, zaključena znanstvena zbirka raziskovalnih podatkov Ključne besede: DNA binding protein, Homo sapiens, synthetic constructs, Escherichia coli Objavljeno v RUNG: 17.03.2021; Ogledov: 2349; Prenosov: 10 Povezava na celotno besedilo |
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5. Development of encapsulation techniques for the production and conservation of synthetic seeds in ornamental plantsYelda Ozden-Tokatli, Anna De Carlo, Fusun Gumusel, Sara Pignattelli, Maurizio Lambardi, 2008, izvirni znanstveni članek Opis: The synthetic seed ("synseed") technology, initially developed through the encapsulation of somatic embryos, is now available for the use with non-embryogenic in vitro-derived explants, such as buds and nodal segments. For ornamental plants, to date there are few reports dealing with the production and the conservation of synseeds. Hence, the present study was conducted to develop an efficient encapsulation protocol for apical and axillary buds from various ornamental shrubs, i.e., oleander (Nerium oleander L.), photinia (Photinia fraseri Dress.), and lilac (Syringa vulgaris L.). For encapsulation, apical and axillary buds were excised, directly immersed in a Na-alginate solution, and then released dropwise in 100 mM CaCl2 center dot 2H(2)O for bead hardening. With photinia, best results were achieved when the synseeds were prepared using 3% Na-alginate, beads hardened for 30 min and germinated on hormone-free or BA-containing gelled MS medium. Thus, up to 92% of synseeds germinated and converted to shoots in a period of 10-11 days. The synthetic seeds of lilac, containing apical buds, showed a shorter germination time when 10-30 g 1(-1) sucrose was included in their "artificial endosperm". Successful medium-term conservation was then achieved with the storage of synthetic seeds at 4 degrees C in the dark on gelled MS medium, where up to 75% (oleander) and and 9 1 % (photinia) synseeds still germinated after 2 or 3 months of cold storage, respectively. Moreover, promising results have been obtained with the cryopreservation of photinia apical buds by means of the technique of "encapsulation-dehydration". Ključne besede: conservation, cryopreservation, encapsulation, ornamentals, synthetic seeds Objavljeno v RUNG: 20.04.2020; Ogledov: 2751; Prenosov: 0 Gradivo ima več datotek! Več... |
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8. NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodiesSandrine Moutel, Nicolas Bery, Virginie Bernard, Laura Keller, Emilie Lemesre, Ario De Marco, Laetitia Ligat, Jean-Christophe Rain, Gilles Fevre, Aurelien Olichon, Franck Perez, 2016, izvirni znanstveni članek Opis: In vitro selection of antibodies allows to obtain highly functional binders, rapidly and at
lower cost. Here, we describe the first fully synthetic phage display library of humanized llama
single domain antibody (NaLi-H1: Nanobody Library Humanized 1). Based on a humanized synthetic
single domain antibody (hs2dAb) scaffold optimized for intracellular stability, the highly diverse
library provides high affinity binders without animal immunization. NaLi-H1 was screened following
several selection schemes against various targets (Fluorescent proteins, actin, tubulin, p53, HP1).
Conformation antibodies against active RHO GTPase were also obtained. Selected hs2dAb were
used in various immunoassays and were often found to be functional intrabodies, enabling tracking
or inhibition of endogenous targets. Functionalization of intrabodies allowed specific protein
knockdown in living cells. Finally, direct selection against the surface of tumor cells produced
hs2dAb directed against tumor-specific antigens further highlighting the potential use of this
library for therapeutic applications. Ključne besede: nanobodies, synthetic phage display library, in vitro panning Objavljeno v RUNG: 17.08.2016; Ogledov: 5109; Prenosov: 243 Celotno besedilo (4,32 MB) |