| Title: | Microfluidic flow injection thermal lens microscopy for high throughput and sensitive analysis of sub-μL samples |
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| Authors: | ID Liu, Mingqiang, UNG (Author)
ID Malovrh, Sara, UNG (Author)
ID Franko, Mladen, UNG (Author) |
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| Files: |  Cr(VI).pdf (828,33 KB)
MD5: DCC2EA043B403CDADC4349647FD888D3
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| Language: | English |
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| Work type: | Not categorized |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | UNG - University of Nova Gorica
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| Abstract: | An analytical method combining microfluidic flow injection analysis (μFIA) with thermal lens microscopy (TLM) was developed for high throughput and sensitive analysis of sub-μL samples. Performance of the μFIA-TLM was validated for detection of hexavalent chromium [Cr(VI)] in water samples. At different sample injection volumes, detection positions and flow rates, influences of the reaction time and the diffusion of Cr-diphenylcarbazone (DPCO) complexes on the μFIA-TLM signal were investigated. Photodegradation of the Cr-DPCO complex was clearly observed when the absorbed photons per Cr-DPCO is above 1600. After optimization of the TLM with respect to rapid flows (up to 10 cm/s), we achieved a limit of detection of 0.6 ng/mL for Cr(VI) in a 50-μm deep channel. Impacts of interfering ions [V(V), Mo(VI), Fe(III)] on the Cr(VI) determination were found to be small. Cr(VI) in real samples from a cement factory were determined and found in good agreement with results of spectrophotometry. This μFIA-TLM shows advantages over its conventional counterpart, such as eliminating additional sample conditioning, reducing over 100 times the sample consumption to sub-μL and over 10 times the time required for one sample injection to a few seconds (up to 20 samples/min). The optimized μFIA-TLM setup can be applied for fast and sensitive analysis of nonfluoresent sub-μL samples in rapidly flowing mediums. |
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| Keywords: | Spektrometrija TLS, mikroskopija TLM, mikrofluidika, krom (VI) |
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| Publication version: | Author Accepted Manuscript |
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| Year of publishing: | 2016 |
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| Number of pages: | 8 |
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| Numbering: | 8 |
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| PID: | 20.500.12556/RUNG-2328  |
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| COBISS.SI-ID: | 4365051 |
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| DOI: | 10.1039/C6AY00932H |
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| NUK URN: | URN:SI:UNG:REP:GJHFAC3A |
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| Publication date in RUNG: | 01.06.2016 |
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| Views: | 6116 |
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| Downloads: | 277 |
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