| Title: | XAS and micro-XRF analysis of mono and bi-metallic exopolysaccharide (FePd-EPS) bio-generated by K. oxytoca |
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| Authors: | ID Arčon, Iztok (Copyright holder)
ID Arčon, Iztok, Univerza v Novi Gorici (Author)
ID Paganelli, Stefano, Università Ca’ Foscari Venezia, Calle Larga Santa Marta 2137, 30123 Venezia, Italy (Author)
ID Piccolo, Oreste, Università Ca’ Foscari Venezia, Calle Larga Santa Marta 2137, 30123 Venezia, Italy (Author)
ID Gallo, Michele, Università Ca’ Foscari Venezia, Calle Larga Santa Marta 2137, 30123 Venezia, Italy (Author)
ID Vogel-Mikuš, Katarina, Biotechnical Faculty, University of Ljubljana, Večna pot 111, 1000 Ljubljana, Slovenia (Author)
ID Baldi, Franco, Università Ca’ Foscari Venezia, Calle Larga Santa Marta 2137, 30123 Venezia, Italy (Author) |
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| Files: |
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| Language: | English |
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| Work type: | Not categorized |
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| Typology: | 1.12 - Published Scientific Conference Contribution Abstract |
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| Organization: | UNG - University of Nova Gorica
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| Abstract: | Bacteria Klebsiella oxytoca (DSM 29614) secret a specific exopolysaccharide (EPS) in the presence of Fe(III)-citrate, as sole carbon and energy source, and produces after 7 days an iron gel precipitate (Fe-EPS) [1]. In the presence of palladium or other metal species, the culture of K. oxytoca can produce other mono or bimetal species (Pd-EPS, FePd-EPS) [2]. These bio-materials may be used as green biogenerated catalysts or for other biotechnological purposes. In this work we present the analysis of the morphology and the chemical state of the metals in mono and bi-metallic (Fe-EPS, Pd-EPS, FePd-EPS) complexes, by a combination of micro X-ray fluorescence, X-ray absorption spectroscopy methods (XANES and EXAFS), and transmission electron microscopy. The results show that iron in monometallic Fe-EPS and bimetalic FePd-EPS is in the mineralized form of iron oxides/hydroxides, predominantly in the form of Fe3+, with a small amount of Fe2+ in the structure, as a mixture of different nano-crystalline iron oxides and hydroxides. Palladium on the other hand is found as Pd(0) in the form of metallic nanoparticles with fcc structure in both, bi-metallic (FePd-EPS) and mono-metallic (Pd-EPS) species. In bi-metallic species Pd and Fe nanoparticles agglomerate in larger clusters, but they remain spatially separated. Access to the SR facilities at ESRF (beamline ID21, project LS-2225), DESY (beamline C) Hamburg (project I-20110511 EC) and ELETTRA (beamline XAFS, project 20115112) is acknowledged. |
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| Keywords: | Klebsiella oxytoca, Fe, Pd, XANES, EXAFS, exopolysaccharide, FePd-EPS |
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| Publication status: | Published |
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| Year of publishing: | 2016 |
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| Number of pages: | 1 |
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| PID: | 20.500.12556/RUNG-2468 |
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| COBISS.SI-ID: | 4416507 |
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| NUK URN: | URN:SI:UNG:REP:HAJODXEC |
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| Publication date in RUNG: | 28.06.2016 |
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| Views: | 6396 |
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| Downloads: | 0 |
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