| Title: | Chelating effect in short polymers for the design of bidentate binders of increased affinity and selectivity |
|---|
| Authors: | ID Fortuna, Sara (Author)
ID Fogolari, Federico (Author)
ID Scoles, Giacinto (Author) |
|---|
| Files: |  12_Fortuna_NHP.pdf (1,68 MB)
MD5: 1DDED2BCD79D84588E0D83C9C713C70C
|
|---|
| Language: | English |
|---|
| Work type: | Not categorized |
|---|
| Typology: | 1.01 - Original Scientific Article |
|---|
| Organization: | UNG - University of Nova Gorica
|
|---|
| Abstract: | The design of new strong and selective binders is a key step towards the development of new sensing devices and effective drugs. Both affinity and selectivity can be increased through chelation and here we theoretically explore the possibility of coupling two binders through a flexible linker. We prove the enhanced ability of double binders of keeping their target with a simple model where a polymer composed by hard spheres interacts with a spherical macromolecule, such as a protein, through two sticky spots. By Monte Carlo simulations and thermodynamic integration we show the chelating effect to hold for coupling polymers whose radius of gyration is comparable to size of the chelated particle. We show the binding free energy of flexible double binders to be higher than that of two single binders and to be maximized when the binding sites are at distances comparable to the mean free polymer end-to-end distance. The affinity of two coupled binders is therefore predicted to increase non linearly and in turn, by targeting two non-equivalent binding sites, this will lead to higher selectivity. |
|---|
| Keywords: | chelation, polymer, multivalency, bidentate, free energy, thermodynamic integration, Monte Carlo |
|---|
| Year of publishing: | 2015 |
|---|
| Number of pages: | 7 |
|---|
| Numbering: | 5 |
|---|
| PID: | 20.500.12556/RUNG-2692  |
|---|
| COBISS.SI-ID: | 4535035 |
|---|
| DOI: | 10.1038/srep15633 |
|---|
| NUK URN: | URN:SI:UNG:REP:KIFILVJK |
|---|
| Publication date in RUNG: | 11.10.2016 |
|---|
| Views: | 4822 |
|---|
| Downloads: | 155 |
|---|
| Metadata: |    |
|---|
|
:
|
Copy citation |
|---|
| | | | Average score: | (0 votes) |
|---|
| Your score: | Voting is allowed only for logged in users. |
|---|
| Share: |  |
|---|
Hover the mouse pointer over a document title to show the abstract or click
on the title to get all document metadata. |
