| Title: | Nanobodies against surface biomarkers enable the analysis of tumor genetic heterogeneity in uveal melanoma Patient Derived Xenografts |
|---|
| Authors: | ID Crepin, Ronan, Institut Curie (Author)
ID Gentien, David, Institut Curie (Author)
ID Duche, Angeline, Institut Curie (Author)
ID Rapinat, Audrey, Institut Curie (Author)
ID Reyes, Cecile, Institut Curie (Author)
ID Nemati, Fariba, Institut Curie (Author)
ID Massonnet, Gerald, Institut Curie (Author)
ID Deacaudin, Didier, Institut Curie (Author)
ID Djander, Selma, Institut Curie (Author)
ID Moutel, Sandrine, Institut Curie (Author)
ID Desrumeaux, Klervi Even, Institut Curie (Author)
ID Cassoux, Nathalie, Institut Curie (Author)
ID Piperno-Neumann, Sophie, Institut Curie (Author)
ID Amigorena, Sebastian, Institut Curie (Author)
ID Perez, Franck, Institut Curie (Author)
ID Roman-Roman, Sergio, Institut Curie (Author)
ID De Marco, Ario, UNG (Author) |
|---|
| Files: |
This document has no files that are freely available to the public. This document may have a physical copy in the library of the organization, check the status via COBISS.  |
|---|
| Language: | English |
|---|
| Work type: | Not categorized |
|---|
| Typology: | 1.01 - Original Scientific Article |
|---|
| Organization: | UNG - University of Nova Gorica
|
|---|
| Abstract: | Monoclonal antibodies specific for biomarkers expressed on the surface of uveal melanoma (UM) cells would simplify the immune-capture and genomic characterization of heterogeneous tumor cells originated from patient derived xenografts (PDXs). Antibodies against four independent tumor antigens were isolated by panning a nanobody synthetic library. Such antibodies enabled flow-cytometry-based sorting of distinct cell sub-populations from UM PDXs and to analyze their genomic features. The complexity and specificity of the biochemical and genomic biomarker combinations mirrored the UM tumor polyclonality. The data showed that MUC18 is highly and universally displayed at the surface of UM cells with different genetic background and consequently represents a reliable pan-biomarker for their identification and purification. In contrast, the other three biomarkers were detected in very variable combinations in UM PDX cells. The availability of the identified nanobodies will be instrumental in developing clone-specific drug evaluation and rational clinical strategies based on accurate genomic profiling |
|---|
| Keywords: | nanobodies, uveal melanoma, patient derived xenografts, MUC18, membrane surface biomarkers, panning, tumor polyclonality |
|---|
| Publication version: | Version of Record |
|---|
| Year of publishing: | 2017 |
|---|
| Number of pages: | 11 |
|---|
| Numbering: | 30 |
|---|
| PID: | 20.500.12556/RUNG-3064-55912669-33a0-6520-c8d7-18453b1b60b4 |
|---|
| COBISS.SI-ID: | 4768763 |
|---|
| DOI: | 10.1111/pcmr.12577 |
|---|
| NUK URN: | URN:SI:UNG:REP:TFTBEVVZ |
|---|
| Publication date in RUNG: | 19.04.2017 |
|---|
| Views: | 6341 |
|---|
| Downloads: | 0 |
|---|
| Metadata: |    |
|---|
|
:
|
Copy citation |
|---|
| | | | Average score: | (0 votes) |
|---|
| Your score: | Voting is allowed only for logged in users. |
|---|
| Share: |  |
|---|
Hover the mouse pointer over a document title to show the abstract or click
on the title to get all document metadata. |
