| Title: | Heparan Sulfate Affects Elastin Deposition in Fibroblasts Cultured from Donors of Different Ages |
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| Authors: | ID Annovi, Giulia, Università di Modena e Reggio Emilia (Author)
ID Boraldi, Federica, Università di Modena e Reggio Emilia (Author)
ID Moscarelli, Pasquale, Università della Basilicata (Author)
ID Guerra, Deanna, Università di Modena e Reggio Emilia (Author)
ID Tiozzo, Roberta, Università di Modena e Reggio Emilia (Author)
ID Parma, Bruna, Opocrin (Author)
ID Sommer, Pascal, Institut de Biologie et Chimie des Prote´ines, CNRS– Universite´ Lyon 1 (Author)
ID Quaglino, Daniela, Università di Modena e Reggio Emilia (Author) |
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| Files: |
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| Language: | English |
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| Work type: | Not categorized |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | UNG - University of Nova Gorica
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| Abstract: | Heparan sulfate (HS), due to its presence on the cell surface and in the extracellular milieu and its ability to
modulate cell signaling, has a fundamental role in both physiological and pathological conditions. For decades
we have demonstrated the occurrence of interactions between glycosaminoglycans (GAGs) and elastic fibers. In
particular, we have recently shown that HS is present inside elastic fibers and plays a role in the assembly and
stability of elastin coacervates. Elastin represents, within the extracellular matrix, the component most severely
affected during aging, and changes in the synthesis and posttranslational modifications of HS have been described,
possibly influencing cellular behavior and protein interactions. Thus, the present study has investigated,
in two different in vitro experimental models, the role of HS on elastin deposition and assembly. Results
demonstrate that: (1) Biological effects of HS are partly dependent on the physicochemical characteristics of the
GAGs; (2) HS does not affect attachment, viability, and growth of human dermal fibroblasts; (3) HS does not
modify elastin gene expression nor elastin synthesis, but favors a-elastin aggregation and, independently from
the age of donors, elastin assembly; (4) HS significantly increases the expression of fibulin 5, and these effects are
especially evident in fibroblasts isolated from aging donors. These data provide a better understanding of the
biological role of HS and offer new perspectives regarding the possibility of restoring and/or preserving the
elastic component with aging. |
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| Keywords: | Heparan sulfate, Elastin, Fibroblasts |
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| Year of publishing: | 2012 |
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| Number of pages: | 22-31 |
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| Numbering: | 15 |
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| PID: | 20.500.12556/RUNG-4697-5731c1ce-7354-e26d-b6fa-16d34a1895da |
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| COBISS.SI-ID: | 5441275 |
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| DOI: | 10.1089/rej.2011.1182 |
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| NUK URN: | URN:SI:UNG:REP:9HFVVJV2 |
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| Publication date in RUNG: | 23.08.2019 |
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| Views: | 2926 |
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| Downloads: | 0 |
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