Title: | Laser ablation-single-particle-inductively coupled plasma mass spectrometry as a multimodality bioimaging tool in nano-based omics |
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Authors: | ID Metarapi, Dino (Author) ID Elteren, Johannes Teun van (Author) ID Šala, Martin (Author) ID Vogel-Mikuš, Katarina (Author) ID Arčon, Iztok (Author) ID Šelih, Vid Simon (Author) ID Kolar, Mitja (Author) ID Hočevar, Samo B. (Author) |
Files: | https://pubs.rsc.org/en/Content/ArticleLanding/2021/EN/D0EN01134G#!divAbstract
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Language: | English |
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Work type: | Unknown |
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Typology: | 1.01 - Original Scientific Article |
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Organization: | UNG - University of Nova Gorica
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Abstract: | Advanced data processing and visualization techniques were applied to laser ablation-single particleinductively
coupled plasma mass spectrometry (LA-sp-ICPMS) data obtained from imaging of roots of
sunflower (Helianthus annuus L.) which were exposed to ionic silver (Ag+). Highly multiplexed images with
tissue level resolution were generated, showing the uptake and transformation of Ag+ to silver
nanoparticles (AgNPs) in whole root cross-sections, and revealing that the size of biosynthesized AgNPs is
predisposed by the reducing power of particular root compartments. Various Ag+ and AgNP visualization
strategies are shown with special emphasis on spatially resolved information associated with the number
and spherical-equivalent size of individual AgNPs in user-selected root cross-sectional regions, including
statistical analysis of the AgNP distribution. Normally, this multimodality information can only be obtained
by combining the results of techniques from several complementary platforms. |
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Keywords: | Laser ablation, Ag XANES, bioimaging |
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Year of publishing: | 2021 |
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Number of pages: | str. 647-656 |
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Numbering: | Vol. 8, iss. 3 |
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PID: | 20.500.12556/RUNG-6567 |
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COBISS.SI-ID: | 50633731 |
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UDC: | 54 |
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ISSN on article: | 2051-8153 |
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DOI: | 10.1039/D0EN01134G |
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NUK URN: | URN:SI:UNG:REP:YRIZCYB0 |
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Publication date in RUNG: | 03.06.2021 |
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Views: | 3071 |
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Downloads: | 51 |
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