| Title: | The molecular toolbox of ectomycorrhizal-driven phosphate mineral weathering |
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| Authors: | ID Paparokidou, Christina, University of Nova Gorica (Author)
ID Jonathan R., Leake, The University of Sheffield (Author)
ID Stephen A., Rolfe, The University of Sheffield (Author)
ID David J., Beerling, The University of Sheffield (Author) |
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| Files: |
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| Language: | English |
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| Work type: | Not categorized |
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| Typology: | 1.12 - Published Scientific Conference Contribution Abstract |
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| Organization: | UNG - University of Nova Gorica
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| Abstract: | Ectomycorrhizal fungi (EMF) form symbiotic partnerships with tree roots and are able to
chemically weather inorganic phosphorus (Pi)-rich minerals, supplying Pi to their host in
return for photosynthates. The fungal-driven nutrient cycling from mineral weathering
plays a pivotal role in ecosystems and crops productivity, as well as geochemical cycles.
The aim of the study presented in this thesis is to unravel the molecular mechanisms by
which the EM fungus Paxillus involutus weathers the Pi-rich mineral hydroxyapatite
(HAP), either non-symbiotically or in symbiosis with its host tree Pinus sylvestris.
Development of an artificial system to study P. involutus responses to varying
concentration of Pi led to the identification of five high-affinity Pi transporter genes, of
which the expression varies in an inversely proportional manner to Pi availability. Using
the same system, whole-transcriptomic data from fungal hyphae unravelled the molecular
basis of the EMF ability in Pi uptake at the global gene scale, revealing that EM symbiosis
can directly affect Pi-responsive fungal genes such as the Pi transporter PiPT4. A second
artificial system was used to study HAP solubilisation driven by P. involutus, which was
confirmed by EDX spectroscopy data showing depletion of Pi from the HAP crystals,
along with secondary minerals formation. Whole-transcriptomic analysis revealed that EM
symbiosis induces a different set of HAP weathering genes in P. involutus hyphae,
compared to the fungus growing non-symbiotically, including the specific expression of
organic acid metabolic genes, which resulted in enhanced HAP solubilisation.
Metabolomic analysis led to the identification of multiple secreted metabolites enriched in
the presence of HAP in P. involutus systems grown non-symbiotically or in symbiosis with
P. sylvestris seedlings. The analysis also led to the identification of putative novel fungal
weathering agents. Results from transcriptomic and metabolomic analyses were
ultimately combined in a model of HAP weathering by P. involutus. |
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| Keywords: | Paxillus involutus, Pinus Sylvestris, ectomycorrhizal symbiosis, mineral weathering, hydroxyapatite, RNA-seq, SEM-EDS, UPLC-Q-TOF-MS |
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| Year of publishing: | 2022 |
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| PID: | 20.500.12556/RUNG-7880 |
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| COBISS.SI-ID: | 140307203 |
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| NUK URN: | URN:SI:UNG:REP:XK9CTLGI |
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| Publication date in RUNG: | 18.01.2023 |
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| Views: | 1913 |
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| Downloads: | 0 |
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