| Naslov: | Microfluidic flow injection thermal lens microscopy for high throughput and sensitive analysis of sub-μL samples |
|---|
| Avtorji: | Liu, Mingqiang (Avtor)
Malovrh, Sara (Avtor)
Franko, Mladen (Avtor) |
|---|
| Datoteke: |  Cr(VI).pdf (828,33 KB)
|
|---|
| Jezik: | Angleški jezik |
|---|
| Vrsta gradiva: | Delo ni kategorizirano (r6) |
|---|
| Tipologija: | 1.01 - Izvirni znanstveni članek |
|---|
| Organizacija: | UNG - Univerza v Novi Gorici |
|---|
| Opis: | An analytical method combining microfluidic flow injection analysis (μFIA) with thermal lens microscopy (TLM) was developed for high throughput and sensitive analysis of sub-μL samples. Performance of the μFIA-TLM was validated for detection of hexavalent chromium [Cr(VI)] in water samples. At different sample injection volumes, detection positions and flow rates, influences of the reaction time and the diffusion of Cr-diphenylcarbazone (DPCO) complexes on the μFIA-TLM signal were investigated. Photodegradation of the Cr-DPCO complex was clearly observed when the absorbed photons per Cr-DPCO is above 1600. After optimization of the TLM with respect to rapid flows (up to 10 cm/s), we achieved a limit of detection of 0.6 ng/mL for Cr(VI) in a 50-μm deep channel. Impacts of interfering ions [V(V), Mo(VI), Fe(III)] on the Cr(VI) determination were found to be small. Cr(VI) in real samples from a cement factory were determined and found in good agreement with results of spectrophotometry. This μFIA-TLM shows advantages over its conventional counterpart, such as eliminating additional sample conditioning, reducing over 100 times the sample consumption to sub-μL and over 10 times the time required for one sample injection to a few seconds (up to 20 samples/min). The optimized μFIA-TLM setup can be applied for fast and sensitive analysis of nonfluoresent sub-μL samples in rapidly flowing mediums. |
|---|
| Ključne besede: | Spektrometrija TLS, mikroskopija TLM, mikrofluidika, krom (VI) |
|---|
| Leto izida: | 2016 |
|---|
| Št. strani: | 8 |
|---|
| Številčenje: | 8 |
|---|
| COBISS_ID: | 4365051  |
|---|
| URN: | URN:SI:UNG:REP:GJHFAC3A |
|---|
| DOI: | 10.1039/C6AY00932H |
|---|
| Število ogledov: | 4413 |
|---|
| Število prenosov: | 261 |
|---|
| Metapodatki: |     |
|---|
| Področja: | Gradivo ni uvrščeno v področja. |
|---|
|
:
|
|
|---|
| | | | Skupna ocena: | (0 glasov) |
|---|
| Vaša ocena: | Ocenjevanje je dovoljeno samo prijavljenim uporabnikom. |
|---|
Postavite miškin kazalec na naslov za izpis povzetka. Klik na naslov izpiše
podrobnosti ali sproži prenos. |