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Naslov:The molecular toolbox of ectomycorrhizal-driven phosphate mineral weathering
Avtorji:ID Paparokidou, Christina, University of Nova Gorica (Avtor)
ID Jonathan R., Leake, The University of Sheffield (Avtor)
ID Stephen A., Rolfe, The University of Sheffield (Avtor)
ID David J., Beerling, The University of Sheffield (Avtor)
Datoteke: Gradivo nima datotek, ki so prostodostopne za javnost. Gradivo je morda fizično dosegljivo v knjižnici fakultete, zalogo lahko preverite v COBISS-u. Povezava se odpre v novem oknu
Jezik:Angleški jezik
Vrsta gradiva:Delo ni kategorizirano
Tipologija:1.12 - Objavljeni povzetek znanstvenega prispevka na konferenci
Organizacija:UNG - Univerza v Novi Gorici
Opis:Ectomycorrhizal fungi (EMF) form symbiotic partnerships with tree roots and are able to chemically weather inorganic phosphorus (Pi)-rich minerals, supplying Pi to their host in return for photosynthates. The fungal-driven nutrient cycling from mineral weathering plays a pivotal role in ecosystems and crops productivity, as well as geochemical cycles. The aim of the study presented in this thesis is to unravel the molecular mechanisms by which the EM fungus Paxillus involutus weathers the Pi-rich mineral hydroxyapatite (HAP), either non-symbiotically or in symbiosis with its host tree Pinus sylvestris. Development of an artificial system to study P. involutus responses to varying concentration of Pi led to the identification of five high-affinity Pi transporter genes, of which the expression varies in an inversely proportional manner to Pi availability. Using the same system, whole-transcriptomic data from fungal hyphae unravelled the molecular basis of the EMF ability in Pi uptake at the global gene scale, revealing that EM symbiosis can directly affect Pi-responsive fungal genes such as the Pi transporter PiPT4. A second artificial system was used to study HAP solubilisation driven by P. involutus, which was confirmed by EDX spectroscopy data showing depletion of Pi from the HAP crystals, along with secondary minerals formation. Whole-transcriptomic analysis revealed that EM symbiosis induces a different set of HAP weathering genes in P. involutus hyphae, compared to the fungus growing non-symbiotically, including the specific expression of organic acid metabolic genes, which resulted in enhanced HAP solubilisation. Metabolomic analysis led to the identification of multiple secreted metabolites enriched in the presence of HAP in P. involutus systems grown non-symbiotically or in symbiosis with P. sylvestris seedlings. The analysis also led to the identification of putative novel fungal weathering agents. Results from transcriptomic and metabolomic analyses were ultimately combined in a model of HAP weathering by P. involutus.
Ključne besede:Paxillus involutus, Pinus Sylvestris, ectomycorrhizal symbiosis, mineral weathering, hydroxyapatite, RNA-seq, SEM-EDS, UPLC-Q-TOF-MS
Leto izida:2022
PID:20.500.12556/RUNG-7880 Novo okno
COBISS.SI-ID:140307203 Novo okno
NUK URN:URN:SI:UNG:REP:XK9CTLGI
Datum objave v RUNG:18.01.2023
Število ogledov:1283
Število prenosov:0
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Gradivo je del monografije

Naslov:SEVENTH INTERNATIONAL SCIENTIFIC CONFERENCE JUNE 5th – WORLD ENVIRONMENT DAY
Uredniki:Merima Toromanovic
Kraj izida:University of Nova Gorica
Leto izida:2022
Prireditelj konference:The University of Bihać

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