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34. PREVENTION AND MANAGEMENT OF LEGIONELLA SPP. SPREAD IN HOSPITAL WATER SYSTEM (ESTABLISHING AN EFFECTIVE SYSTEM WITHOUT USING CHEMICALS IN UNIVERSITY CLINIC OF RESPIRATORY AND ALLERGIC DISEASES GOLNIK)Mojca Novak, 2016, master's thesis Abstract: The hospital water system can be a causative agent for acquiring hospital-acquired infection. Many microorganisms can be detected in potable water and one of this is Legionella spp. These bacteria live also in nature, but are usually present in low numbers. When entering into the buildings and their water supply, Legionella finds ideal conditions to multiply, especially if this systems are extensive and old. To control the conditions in the levels that cannot harm humans, many procedures – physical and chemical – have been established. All of them have advantages as well as limitations. According to these facts, in University Clinic of Respiratory and Allergic Diseases Golnik we tried to reestablish the protocols that would keep us on the safe side and be environmentally and health friendly as well. We have been trying to use the protocol without added chemicals – just with maintaining proper temperatures, heat disinfection and flushing for many years now. With this study we wanted to extend our knowledge of our hospital water system, so we intensified the sampling and introduced extended measures. We found out that the presence of Legionella in water system is connected to moderate temperatures and it is present more frequently in high risk zones (inappropriate temperatures, dead legs, water stagnation). With constant measures, the Legionella can be controlled but not eradicated. To contain it on a safe level, the group of experts must work together and introduce procedures that will provide safe environment for the patients and employees.
Keywords: Legionella spp., hospital water system, prevention, temperature, flushing.
Published in RUNG: 30.09.2016; Views: 6156; Downloads: 276
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35. Poetični jezik in ponavljanjeMojca Pretnar, 2016, master's thesis Abstract: Delo Poetični jezik in ponavljanje se loteva razmerja med poetičnim jezikom in navadnim jezikom skozi psihoanalitične koncepte. Poetični jezik, kakor ga vidi psihoanaliza, je neločljivi del jezika in temelji na prepletu Lacanovih treh registrov: simbolnega, imaginarnega in realnega - tudi poetični jezik strukturo boromejskega vozla. Poetični jezik, ki ga najdemo v poetičnih besedilih, v primerjavi z navadnim jezikom pomeni spremembo v kodu, ustvarja kršitve in se od navadnega jezika loči po večjem deležu realnega, ki proizvaja različne učinke, zaradi katerih posegamo po poetičnih besedilih.
Jezik se konstituira skozi ponavljanje in ponavljanje je moč jezika. Ponavljanje je vse prej kot zaviralna sila, ravno ponavljanje ponuja možnost, da se ustvarja novo. Pretresanje poetičnega jezika skozi dva različna koncepta ponavljanja, ponavljanje, kakor ga vidita Lacanova psihoanaliza in Deleuzova filozofija, potrdi, da se poetični jezik konstituira skozi vse plati ponavljanja. Če poetični jezik proizvaja realne učinke in stvori umetniško poetično besedilo, sestoji iz ponavljanja v vseh treh registrih po Lacanovi tipologiji ali mora biti sklop vseh treh sintez po Deleuzovi tipologiji. To vednost mora v zakup vzeti tudi prevajanje poetičnih besedil, ki je tudi en primer ponavljanja.
Keywords: poetični jezik, jejezik, boromejski vozel, ponavljanje v psihoanalizi, Deleuzov koncept ponavljanja, objekt a, literarno prevajanje
Published in RUNG: 28.09.2016; Views: 7147; Downloads: 275
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36. Italijanski vrabec (Passer italiae) v Vipavski doliniMojca Grmek, 2016, undergraduate thesis Abstract: V diplomskem delu smo raziskovali prisotnost odraslih samcev domačega vrabca (Passer domesticus Linnaeus, 1758) in italijanskega vrabca (Passer italiae Vieillot, 1817) ter križancev po naseljih v Vipavski dolini. Zanimalo nas je trenutno stanje, s pomočjo literature smo opisali tudi trend populacije italijanskih vrabcev v Vipavski dolini. Ugotovili smo, da delež odraslih samcev italijanskega vrabca v primerjavi z odraslimi samci domačega vrabca po naseljih od zahoda proti vzhodu Vipavske doline upada in da se je trend širjenja populacije italijanskih vrabcev po Vipavski dolini stabiliziral. Prav tako smo prišli do zaključka, da domači in italijanski vrabci med kmečkimi in mestnimi zgradbami ne izbirajo, temveč jih poseljujejo v skladu z razmerjem v naseljih.
Keywords: italijanski vrabec (Passer italiae), domači vrabec (Passer domesticus), populacijska dinamika, Vipavska dolina
Published in RUNG: 07.09.2016; Views: 5318; Downloads: 291
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37. Determination of biogenic amines by thermal lens microscopic detection of enzymatically released ammoniumMojca Žorž, Mladen Franko, 2016, published scientific conference contribution abstract Abstract: Biogenic amines (BAs) are organic amines present in meat, fish, dairy produce and wine due to the breakdown of amino acids, catalysed by microbial decarboxylases. BAs determination in food is important not only because of possible toxicological effects such as nausea, sweating and headache but also due to their possible role as indicators of food spoilage. Chromatographic methods are traditionally applied for determination of BAs in food [1], which usually require preliminary operations for sample pre-treatment that are laborious and difficult to automate. On the other hand, screening analytical systems provide simple, low cost and rapid analysis with the possibility of subjecting high number of samples to the screening system in a short time [2]. In this work we present a novel method for screening determination of BAs using a microfluidic system with the detection by highly sensitive thermal lens microscope (μFIA-TLM).
Four biogenic amines (putrescine, cadaverine, histamine and tyramine) were subjected to enzymatic catalysis by transglutaminase, where ammonia was released as a product of acyl transfer reaction between the peptide bound glutamine (Gln) and the amino group of BAs. Ammonia was further transformed into indophenol blue by the Berthelot reaction. The coloured product was detected in batch (static) mode in a 100 μm sample cell or in μFIA (flowing) mode in a microchip with the same optical path length. The detection was performed on a TLM system applying a solid-state diode as an excitation source (660 nm). Organic solvents were tested for signal enhancement.
For evaluation of the sensitivity and determination of LOD values (S/N = 3 basis), the NH4Cl standard solution was applied in Berthelot reaction with further detection on TLM system. The LODs for NH4+ in batch mode and in μFIA were 24 μg/L and 109 μg/L, respectively. Both LOD values are lower than the LOD achieved with conventional spectrophotometry (180 μg/L). When mixtures of the indophenol standard solutions and EtOH in the ratio of 1:1 were prepared the LOD in batch mode was improved to 3 μg/L, achieving 60-times improvement compared to spectrophotometry.
Keywords: Biogenic amines, Thermal lens microscopy, microfluidics, translgutaminase
Published in RUNG: 05.07.2016; Views: 5160; Downloads: 0
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40. Decision support in the implementation of sustainable development in protected areas regarding environmental education and ecotourism : dissertationMojca Stubelj Ars, 2014, doctoral dissertation Keywords: podpora pri odločanju, zavarovana območja, trajnost, okoljska vzgoja, ekoturizem, okolju prijazno vedenje, varstvo narave, modeli, disertacije
Published in RUNG: 25.04.2014; Views: 5344; Downloads: 431
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