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1.
NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies
Jean-Christophe Rain, Laetitia Ligat, Ario de Marco, Emilie Lemesre, Laura Keller, Virginie Bernard, Nicolas Bery, Sandrine Moutel, Gilles Fevre, Aurelien Olichon, Franck Perez, 2016, original scientific article

Abstract: In vitro selection of antibodies allows to obtain highly functional binders, rapidly and at lower cost. Here, we describe the first fully synthetic phage display library of humanized llama single domain antibody (NaLi-H1: Nanobody Library Humanized 1). Based on a humanized synthetic single domain antibody (hs2dAb) scaffold optimized for intracellular stability, the highly diverse library provides high affinity binders without animal immunization. NaLi-H1 was screened following several selection schemes against various targets (Fluorescent proteins, actin, tubulin, p53, HP1). Conformation antibodies against active RHO GTPase were also obtained. Selected hs2dAb were used in various immunoassays and were often found to be functional intrabodies, enabling tracking or inhibition of endogenous targets. Functionalization of intrabodies allowed specific protein knockdown in living cells. Finally, direct selection against the surface of tumor cells produced hs2dAb directed against tumor-specific antigens further highlighting the potential use of this library for therapeutic applications.
Found in: ključnih besedah
Summary of found: ...In vitro selection of antibodies allows to obtain highly...
Keywords: nanobodies, synthetic phage display library, in vitro panning
Published: 17.08.2016; Views: 1890; Downloads: 149
.pdf Fulltext (4,32 MB)

2.
Nanobody technology: principles & applications
Ario De Marco, invited lecture at foreign university

Abstract: Antibodies possess unattainable capacity to bind selectively and at high affinity their cognates. For this reason they have been largely used in applications which rely on specific molecular recognition. Biosensors, nanoparticles, and even cells can be functionalized with antibodies for improving sensitivity and target specificity. However, conventional antibodies (IgGs) are large molecules (150 kDa) that are difficult to engineer. In the last years, antibody fragments have become more and more popular as an effective alternative and specifically nanobodies raised enthusiasm because of their minimal mass (14 kDa), high stability, relative similarity to human sequences, and simplified mutagenesis. Pre-immune nanobody libraries have the further advantage of enabling blind selection for antigens that can be used to discriminate between subpopulations of cells and vesicles. The panning can be performed directly on intact cells and the resulting binders are specific for the native antigen conformation
Found in: ključnih besedah
Summary of found: ...Nanobodies, in vitro panning, cancer biomarkers, recombinant antibody engineering...
Keywords: Nanobodies, in vitro panning, cancer biomarkers, recombinant antibody engineering
Published: 03.05.2017; Views: 2025; Downloads: 0
.pdf Fulltext (37,92 KB)

3.
Nanobodies: flexible structures for biotechnological applications
Ario De Marco, unpublished invited conference lecture

Found in: ključnih besedah
Summary of found: ...nanobodies, in vitro panning, extracellular vesicles, oncology, toxic algae...
Keywords: nanobodies, in vitro panning, extracellular vesicles, oncology, toxic algae
Published: 16.03.2018; Views: 968; Downloads: 0
.pdf Fulltext (436,52 KB)

4.
Pre-immune nanobody collections and their biotechnological applications
Ario De Marco, invited lecture at foreign university

Found in: ključnih besedah
Summary of found: ...phage libraries, nanobodies, in vitro antibody panning...
Keywords: phage libraries, nanobodies, in vitro antibody panning
Published: 17.09.2018; Views: 586; Downloads: 0
.pdf Fulltext (54,45 KB)

5.
In vitro and in silico approaches to obtain tailored reagents – Nanobodies as model
Ario De Marco, invited lecture at foreign university

Found in: ključnih besedah
Summary of found: ...Immunoreagents, nanobodies, in vitro panning, recombinant antibody production...
Keywords: Immunoreagents, nanobodies, in vitro panning, recombinant antibody production
Published: 21.09.2018; Views: 585; Downloads: 0
.pdf Fulltext (979,15 KB)

6.
Nanobodies: How to Exploit Pre-Immune Libraries and Recombinant Antibody Fragments
Ario De Marco, unpublished invited conference lecture

Found in: ključnih besedah
Summary of found: ...nanobodies, in vitro panning, phage display...
Keywords: nanobodies, in vitro panning, phage display
Published: 23.08.2019; Views: 436; Downloads: 0
.pdf Fulltext (188,41 KB)

7.
Pre-immune libraries of recombinant nanobodies: outcome from 15-year experience
Ario De Marco, invited lecture at foreign university

Found in: ključnih besedah
Summary of found: ...Nanobodies, in vitro panning, protein functionalization...
Keywords: Nanobodies, in vitro panning, protein functionalization
Published: 14.02.2020; Views: 243; Downloads: 0
.pdf Fulltext (126,22 KB)

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