251. Coupling of autoionizing states by a chirped laser pulseMatjaž Žitnik, Andrej Mihelič, Klemen Bučar, Mateja Hrast, Žiga Barba, Špela Krušič, Primož Rebernik Ribič, Jurij Urbančič, Barbara Ressel, Matija Stupar, David Gauthier, Giovanni De Ninno, 2020, published scientific conference contribution abstract Abstract: We have observed the autoionization of the laser-coupled 2s2p 1Po and 2p2 1Se resonances in helium. The ions were collected while varying the frequency and delay of the extreme-ultraviolet (EUV) excitation pulse with respect to the linearly chirped visible (VIS) laser pulse. From the measured frequency-delay map the Autler- Townes splitting, the EUV-VIS cross-correlation and the linear chirp parameter were extracted.
Keywords: High Harmonic Generation
Published in RUNG: 29.06.2020; Views: 3194; Downloads: 0
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252. Search for magnetically-induced signatures in the arrival directions of ultra-high-energy cosmic rays measured at the Pierre Auger ObservatoryA. Aab, Andrej Filipčič, Gašper Kukec Mezek, Samo Stanič, Marta Trini, Serguei Vorobiov, Danilo Zavrtanik, Marko Zavrtanik, Lukas Zehrer, 2020, original scientific article Keywords: ultra-high-energy cosmic rays (UHECR), Pierre Auger Observatory, UHECR arrival directions, galactic magnetic field, UHECR event multiplets
Published in RUNG: 19.06.2020; Views: 2628; Downloads: 0
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253. Implementation of high performance liquid chromatography coupled to thermal lens spectrometry (HPLC‑TLS) for quantification of pyranoanthocyanins during fermentation of Pinot Noir grapesJelena Topič Božič, Lorena Butinar, Natka Ćurko, Karin Kovačević Ganić, Branka Mozetič Vodopivec, Dorota Korte, Mladen Franko, 2020, original scientific article Abstract: In this work high performance liquid chromatography coupled to thermal lens spectrometry (HPLC-TLS) was applied for monitoring of vinylphenolic pyranoanthocyanins formation during the fermentation of Pinot Noir wines. Vinylphenolic pyranoanthocyanins are wine pigments, present in low concentrations, but very important for wine colour stability.
Fermentation process was conducted with four different yeast strains, used as starters, either in sequential fermentation of non-Saccharomyces with S. cerevisiae yeast or as single fermentation with S. cerevisiae yeasts in order to test the applicability of developed method for monitoring of selected compounds in real wine fermentation experiments. The
developed HPLC-TLS method showed higher sensitivity compared to HPLC coupled to diode array detection (DAD) technique for particular wine colour compounds. Obtained limits of detection (LODs), were 6- and 22-times lower in comparison to HPLC–DAD in gradient and isocratic elution mode, respectively, whereas limits of quantification (LOQs)
5 and 18-times lower. Lower LODs enabled earlier observation of vinylphenolic pyranoanthocyanins formation during fermentation (already at day 7) in the case of HPLC-TLS method in gradient mode, while by using HPLC–DAD in gradient elution mode the formation of vinylphenolic pyranoanthocyanins was noticed only after 12 days of fermentation.
Keywords: Thermal lens spectrometry (TLS), High performance liquid chromatography (HPLC), Pyranoanthocyanins, Wine, Yeasts
Published in RUNG: 18.06.2020; Views: 3365; Downloads: 0
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254. DEVELOPMENT OF A NOVEL PCR-BASED ASSAY FOR HIGH-RISK HUMAN PAPILLOMAVIRUS DETECTION AND GENOTYPING IN SELF COLLECTED CERVICOVAGINAL SAMPLES: A NEW POSSIBILITY FOR THE CERVICAL CANCER SCREENINGAlice Avian, 2020, doctoral dissertation Abstract: Human Papillomavirus (HPV) infection is the causative agent for the invasive cervical cancer and its precancerous lesions, furthermore, there are growing evidences of HPV being a relevant factor in other anogenital cancers as well as head and neck cancers. Most sexually active women become infected with HPV at least once in their lifetime, but less than 10% of women becomes persistently infected, and it is precisely the persistent infection that contributes to the development of cervical cancer.
The preventive effect of cervical cancer screening largely depends in the high women participation and coverage; indeed, a large number of cervical cancers diagnoses normally arise among under-screened and unscreened women. Increase in the screening coverage is essential to improve the effectiveness of cervical screening programmes.
The main purpose of this PhD project was to solve some of the most relevant problems in the cervical cancer screening programmes, as the increase of cost-effectiveness and the amelioration of the screening coverage.
My work was focused on the development and validation of the first Ulisse BioMed S.p.A. product, the HPV Selfy™ test, an innovative PCR-based kit for the direct detection and genotyping of 12 high-risk HPV types (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58 and 59) and 2 possible/probable high-risk (66 and 68), specifically optimized for the analysis of self-collected vaginal specimens.
The core of this innovative test is based on high-resolution melting (HRM) analysis, a recently developed technique for fast, high-throughput post-PCR analysis of variance in nucleic acid sequences, that characterizes the amplicons by studying thermal denaturation of double-stranded DNA. Based on this approach and through the design of different HPV type-specific primer pairs and the development of a specific master mix, unique melting peaks in a single fluorescence channel were obtained, allowing the multiple detection and genotyping of 14 HPV types in a single PCR well.
Three different clinical studies have been carried out to validate the assay on the vaginal self-collected samples with truly amazing results regarding the assay’s performance, but also for self-sampling acceptability by women. Moreover, data collected in these studies suggest a future possible use of this test for the hard-to-reach women, as an alternative of the conventional clinician-collected sample, in order to increase the cervical cancer screening coverage.
Keywords: Human Papillomavirus, HPV test, cervical cancer screening, prevention, diagnostic test, High resolution melting, HRM, genotyping, PCR, Self-sampling, clinical validation.
Published in RUNG: 17.06.2020; Views: 3562; Downloads: 106
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255. Social Impact of Outreach at the Pierre Auger ObservatoryBeatriz García, Andrej Filipčič, Gašper Kukec Mezek, Samo Stanič, Marta Trini, Serguei Vorobiov, Lili Yang, Danilo Zavrtanik, Marko Zavrtanik, Lukas Zehrer, 2019, published scientific conference contribution Keywords: ultra-high energy cosmic rays, Pierre Auger Observatory, outreach, social impact
Published in RUNG: 16.06.2020; Views: 2499; Downloads: 77
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256. The Energy Scale of the Pierre Auger ObservatoryBruce R. Dawson, Andrej Filipčič, Gašper Kukec Mezek, Samo Stanič, Marta Trini, Serguei Vorobiov, Lili Yang, Danilo Zavrtanik, Marko Zavrtanik, Lukas Zehrer, 2019, published scientific conference contribution Keywords: ultra-high energy cosmic rays, Pierre Auger Observatory, energy scale
Published in RUNG: 16.06.2020; Views: 2469; Downloads: 73
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257. Measurement of the cosmic ray flux near the second knee with the Pierre Auger ObservatoryAlan Coleman, Andrej Filipčič, Gašper Kukec Mezek, Samo Stanič, Marta Trini, Serguei Vorobiov, Lili Yang, Danilo Zavrtanik, Marko Zavrtanik, Lukas Zehrer, 2019, published scientific conference contribution Keywords: ultra-high energy cosmic rays (UHECR), UHECR energy spectrum, second knee, Pierre Auger Observatory
Published in RUNG: 16.06.2020; Views: 2660; Downloads: 79
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258. Long Term Performance of the Pierre Auger ObservatoryKoun Choi, Andrej Filipčič, Gašper Kukec Mezek, Samo Stanič, Marta Trini, Serguei Vorobiov, Lili Yang, Danilo Zavrtanik, Marko Zavrtanik, Lukas Zehrer, 2019, published scientific conference contribution Keywords: ultra-high energy cosmic rays, Pierre Auger Observatory, long term performance
Published in RUNG: 16.06.2020; Views: 2584; Downloads: 82
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259. Anisotropies of the highest energy cosmic-ray events recorded by the Pierre Auger Observatory in 15 years of operationLorenzo Caccianiga, Andrej Filipčič, Gašper Kukec Mezek, Samo Stanič, Marta Trini, Serguei Vorobiov, Lili Yang, Danilo Zavrtanik, Marko Zavrtanik, Lukas Zehrer, 2019, published scientific conference contribution Keywords: ultra-high energy cosmic rays (UHECR), UHECR anisotropies, Pierre Auger Observatory
Published in RUNG: 16.06.2020; Views: 2652; Downloads: 100
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260. Highlights from the Pierre Auger ObservatoryAntonella Castellina, Andrej Filipčič, Gašper Kukec Mezek, Samo Stanič, Marta Trini, Serguei Vorobiov, Lili Yang, Danilo Zavrtanik, Marko Zavrtanik, Lukas Zehrer, 2019, published scientific conference contribution Keywords: ultra-high energy cosmic rays, extensive air showers, Pierre Auger Observatory
Published in RUNG: 16.06.2020; Views: 3105; Downloads: 101
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