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4842. Determination of petrophysical properties of sedimentary rocks by optical methodsDorota Korte, Dejan Kaukler, Mattia Fanetti, Humberto Cabrera, Efrain Daubronte, Mladen Franko, 2017, original scientific article Keywords: sedimentary rocks, beam deflection spectroscopy, grain size distribution, petrophysical
properties, chemical composition
Published in RUNG: 06.02.2017; Views: 4285; Downloads: 0
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4843. Didaktični model učne verige odlomkov: na primeru novele Tantadruj Cirila KosmačaZoran Božič, 2016, original scientific article Abstract: Članek obravnava primer šolske interpretacije srednje dolgega pripovednega besedila, in sicer po modelu učne verige odlomkov z vprašanji za tesno branje. Ta didaktični pristop se je sicer na Slovenskem uveljavljal že pred drugo svetovno vojno, bolj sistematično uresničitev pa je doživel z izidom delovnih zvezkov za domače branje Zlati poljub in Poljub zlata leta 1998. Po skoraj dveh desetletjih šolske rabe je čas za natančnejšo presojo pristopa oz. njegove uporabnosti, tudi s pomočjo primerjave dveh učnih verig, nastalih po znani Kosmačevi noveli Tantadruj. Članek zaključuje primer učne verige, ki Tantadrujevo iskanje sreče
povezuje s pripovedovalčevim iskanjem ustvarjalnega navdiha.
Keywords: Ciril Kosmač, učna veriga, tesno branje, domače branje, didaktika književnosti
Published in RUNG: 06.02.2017; Views: 4814; Downloads: 0
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4846. Electron Microscopic Visualization of Complementary Labeled DNA With Platinum-Containing Guanine DerivativeAlexandre Loukanov, Chavdar Filipov, Polina Mladenova, Svetlin TOSHEV, Saim Emin, 2016, original scientific article Abstract: The object of the present report is to provide a method for a visualization of DNA in TEM by complementary labeling of cytosine with guanine derivative, which contains platinum as contrast-enhanced heavy element. The stretched single-chain DNA was obtained by modifying double-stranded DNA. The labeling method comprises the following steps: (i) stretching and adsorption of DNA on the support film of an electron microscope grid (the hydrophobic carbon film holding negative charged DNA); (ii) complementary labeling of the cytosine bases from the stretched single-stranded DNA pieces on the support film with platinum containing guanine derivative to form base-specific hydrogen bond; and (iii) producing a magnified image of the base-specific labeled DNA. Stretched single-stranded DNA on a support film is obtained by a rapid elongation of DNA pieces on the surface between air and aqueous buffer solution. The attached platinum-containing guanine derivative serves as a high-dense marker and it can be discriminated from the surrounding background of support carbon film and visualized by use of conventional TEM observation at 100 kV accelerated voltage. This method allows examination of specific nucleic macromolecules through atom-by-atom analysis and it is promising way toward future DNA-sequencing or molecular diagnostics of nucleic acids by electron microscopic observation.
Keywords: DNA stretching and visualization, contrast-enhanced Pt-containing guanine, DNA sequences
Published in RUNG: 02.02.2017; Views: 4848; Downloads: 0
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4847. Biotinylated Vanadium and Chromium Sulfide Nanoparticles as Probes for Colocalization of Membrane ProteinsAlexandre Loukanov, Saim Emin, 2016, original scientific article Abstract: ABSTRACT We report the microemulsion synthesis of vanadium and chromium sulfide nanoparticles (NPs) and their biological application as nanoprobes for colocalization of membrane proteins. Spherical V2S3 and Cr2S3 NPs were prepared in reverse microemulsion droplets, as nanoreactors, obtained by the surfactant sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in nonpolar organic phase (heptane). Electron microscopic data indicated that the size distribution of the nanoparticles was uniform with an average diameter between 3 4 5 nm. The prepared hydrophobic nanocrystals were transferred in aqueous phase by surface cap exchange of AOT with biotindihydrolipoic ligands. This substitution allows the nanoparticles solubility in aqueous solutions and confer their bioactivity. In addition, we report the conjugation procedure between a-Lipoic acid (LA) and biotin (abbreviated as biotin-LA). The biotin-LA structure was characterized by 1D and 2D NMR spectroscopy. The biotinylated vanadium and chromium sulfide nanoparticles were tested as probes for colocalization of glutamate receptors on sodium-dodecyl-sulfate-digested replica prepared from rat hippocampus. The method suggests their high labeling efficiency for study of membrane biological macromolecules.
Keywords: biotin-dihydrolipoic conjugate, vanadium and chromium sulfide nanoparticles, membrane proteins
Published in RUNG: 02.02.2017; Views: 5294; Downloads: 0
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4849. Studies towards an understanding of global array pointing for the Cherenkov Telescope ArrayStefan Eschbach, Andrej Filipčič, Gašper Kukec Mezek, Samo Stanič, Serguei Vorobiov, Lili Yang, Gabrijela Zaharijas, Danilo Zavrtanik, Marko Zavrtanik, 2015, published scientific conference contribution Keywords: Cherenkov Telescope Array (CTA), CTA global array pointing, point-source location accuracy, Monte Carlo simulations
Published in RUNG: 26.01.2017; Views: 3979; Downloads: 247
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4850. Design of a prototype device to calibrate the Large Size Telescope camera of the Cherenkov Telescope ArrayM. Iori, Andrej Filipčič, Gašper Kukec Mezek, Samo Stanič, Serguei Vorobiov, Lili Yang, Gabrijela Zaharijas, Danilo Zavrtanik, Marko Zavrtanik, 2015, published scientific conference contribution Keywords: Cherenkov Telescope Array (CTA), large-size telescope (LST) design for CTA, LST camera calibration
Published in RUNG: 26.01.2017; Views: 3566; Downloads: 212
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