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31.
Urban tracer dispersion experiments during the second DAPPLE field campaign in London 2004
Damien Martin, Catheryn S Price, Iain R. White, Graham Nickless, K Fredrik Petersson, Rex E Britter, Alan G Robins, Stephen E Belcher, Janet F Barlow, Marie Neophytou, Samantha J Arnold, Alan S Tomlin, Robert J Smalley, Dudley E. Shallcross, 2010, original scientific article

Abstract: As part of the DAPPLE programme two large scale urban tracer experiments using multiple simultaneous releases of cyclic perfluoroalkanes from fixed location point sources was performed. The receptor concentrations along with relevant meteorological parameters measured are compared with a three screening dispersion models in order to best predict the decay of pollution sources with respect to distance. It is shown here that the simple dispersion models tested here can provide a reasonable upper bound estimate of the maximum concentrations measured with an empirical model derived from field observations and wind tunnel studies providing the best estimate. An indoor receptor was also used to assess indoor concentrations and their pertinence to commonly used evacuation procedures.
Keywords: Dapple, dispersion
Published in RUNG: 18.07.2019; Views: 2826; Downloads: 0
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32.
Dispersion experiments in central London: The 2007 DAPPLE project
Curtis R Wood, Samantha J Arnold, Ahmed A Balogun, Janet F Barlow, Stephen E Belcher, Rex E Britter, Hong Cheng, Adrian Dobre, Justin J N Lingard, Damien Martin, Marina K Neophytou, Fredrik K Petersson, Alan G Robins, Dudley E. Shallcross, Robert J Smalley, James E Tate, Alison S Tomlin, Iain R. White, 2009, original scientific article

Abstract: In the event of a release of toxic gas in the center of London, emergency services personnel would need to determine quickly the extent of the area contaminated. The transport of pollutants by turbulent flow within the complex streets and building architecture of London, United Kingdom, is not straightforward, and we might wonder whether it is at all possible to make a scientifically reasoned decision. Here, we describe recent progress from a major U.K. project, Dispersion of Air Pollution and its Penetration into the Local Environment (DAPPLE; information online at www.dapple.org.uk). In DAPPLE, we focus on the movement of airborne pollutants in cities by developing a greater understanding of atmospheric flow and dispersion within urban street networks. In particular, we carried out full-scale dispersion experiments in central London from 2003 through 2008 to address the extent of the dispersion of tracers following their release at street level. These measurements complemented previous studies because 1) our focus was on dispersion within the first kilometer from the source, when most of the material was expected to remain within the street network rather than being mixed into the boundary layer aloft; 2) measurements were made under a wide variety of meteorological conditions; and 3) central London represents a European, rather than North American, city geometry. Interpretation of the results from the full-scale experiments was supported by extensive numerical and wind tunnel modeling, which allowed more detailed analysis under idealized and controlled conditions. In this article, we review the full-scale DAPPLE methodologies and show early results from the analysis of the 2007 field campaign data.
Keywords: Air quality, Atmospheric thermodynamics, Dispersions, Experiments
Published in RUNG: 18.07.2019; Views: 3999; Downloads: 0
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34.
Heptapeptides as potential neutralizers for Shiga toxin 1 cytotoxicity.
Robert Alvin Bernedo-Navarro, Mayara Mayele Miyachiro, Yano Tomomasa, unpublished conference contribution

Keywords: Shiga toxins, peptide phage display, neutralizers
Published in RUNG: 05.06.2019; Views: 3614; Downloads: 0
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35.
Functional and structural characterization of nanobodies neutralizing Shiga toxin 2
Robert Alvin Bernedo Navarro, Ema Romao, Tomomasa Yano, Yann Sterckx, Serge Muyldermans, unpublished conference contribution

Keywords: Shiga toxins, nanobodies, Stx2
Published in RUNG: 05.06.2019; Views: 3234; Downloads: 0
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36.
Nanoanticorpos neutralizantes das toxinas de Shiga
Bernedo Navarro Robert Alvin, unpublished invited conference lecture

Keywords: Nanobodies, Shiga toxins
Published in RUNG: 05.06.2019; Views: 2972; Downloads: 0
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37.
Phosphorylation of HPV-16 L2 Contributes To Efficient Virus Infectious Entry
Justyna Broniarczyk, Paola Massimi, David Pim, Martina Bergant Marušič, Michael P. Myers, Robert L. Garcea, Lawrence Banks, 2019, original scientific article

Abstract: The Human Papillomavirus (HPV) capsid comprises two viral proteins, L1 and L2, with the L2 component being essential to ensure efficient endocytic transport of incoming viral genomes. Several studies have previously reported that L1 and L2 are post-translationally modified, but it is uncertain whether these modifications affect HPV infectious entry. Using a proteomic screen, we identified a highly conserved phospho-acceptor site on the HPV-16 and BPV-1 L2 proteins. The phospho-modification of L2, and its presence in HPV pseudovirions (PsVs), was confirmed using anti-phospho L2-specific antibodies. Mutation of the phospho-acceptor sites of both HPV-16 and BPV-1 L2 resulted in the production of infectious virus particles, with no differences in efficiency of packaging the reporter DNA. However, these mutated PsVs showed marked defects in infectious entry. Further analysis revealed a defect in uncoating, characterized by a delay in the exposure of a conformational epitope on L1 that indicates capsid uncoating. This uncoating defect was accompanied by a delay in the proteolysis of both L1 and L2 in mutated HPV-16 PsVs. Taken together, these studies indicate that phosphorylation of L2 during virus assembly plays an important role in optimal uncoating of virions during infection, suggesting that phosphorylation of the viral capsid proteins contributes to infectious entry.
Keywords: HPV, L2, infection, protein phosphorylation
Published in RUNG: 05.06.2019; Views: 3433; Downloads: 0
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