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Nanobodies against surface biomarkers enable the analysis of tumor genetic heterogeneity in uveal melanoma Patient Derived Xenografts
Ronan Crepin, David Gentien, Angeline Duche, Audrey Rapinat, Cecile Reyes, Fariba Nemati, Gerald Massonnet, Didier Deacaudin, Selma Djander, Sandrine Moutel, Klervi Even Desrumeaux, Nathalie Cassoux, Sophie Piperno-Neumann, Sebastian Amigorena, Franck Perez, Sergio Roman-Roman, Ario de Marco, 2017, original scientific article

Abstract: Monoclonal antibodies specific for biomarkers expressed on the surface of uveal melanoma (UM) cells would simplify the immune-capture and genomic characterization of heterogeneous tumor cells originated from patient derived xenografts (PDXs). Antibodies against four independent tumor antigens were isolated by panning a nanobody synthetic library. Such antibodies enabled flow-cytometry-based sorting of distinct cell sub-populations from UM PDXs and to analyze their genomic features. The complexity and specificity of the biochemical and genomic biomarker combinations mirrored the UM tumor polyclonality. The data showed that MUC18 is highly and universally displayed at the surface of UM cells with different genetic background and consequently represents a reliable pan-biomarker for their identification and purification. In contrast, the other three biomarkers were detected in very variable combinations in UM PDX cells. The availability of the identified nanobodies will be instrumental in developing clone-specific drug evaluation and rational clinical strategies based on accurate genomic profiling
Found in: osebi
Keywords: nanobodies, uveal melanoma, patient derived xenografts, MUC18, membrane surface biomarkers, panning, tumor polyclonality
Published: 19.04.2017; Views: 4262; Downloads: 0
.pdf Fulltext (1,11 MB)

Nanobodies as biotechnological tools
Ario de Marco, invited lecture at foreign university

Found in: osebi
Keywords: nanobodies, pre-immune phage library, recombinant expression, biomarkers, tumors
Published: 17.05.2017; Views: 2586; Downloads: 0
.pdf Fulltext (290,82 KB)

Acting of folding effectors to improve recombinant protein yields and functional quality
Ario de Marco, 2017, independent scientific component part or a chapter in a monograph

Found in: osebi
Keywords: recombinant proteins, chaperones, osmolytes, protein aggregation
Published: 24.05.2017; Views: 2964; Downloads: 0
.pdf Fulltext (9,50 MB)

Nuovi modelli di ricerca: oncologia comparativa per lo studio dei sarcomi
Ario de Marco, unpublished invited conference lecture

Found in: osebi
Keywords: Comparative oncology, sarcoma, canine model, immune-reagents
Published: 10.10.2017; Views: 2499; Downloads: 0
.pdf Fulltext (173,81 KB)

A caccia di proteine usando gli anticorpi come esca
Ario de Marco, unpublished invited conference lecture

Found in: osebi
Keywords: Fluorescent proteins, antibodies, microscopy, diagnostics
Published: 13.02.2018; Views: 2377; Downloads: 0
.pdf Fulltext (215,78 KB)

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