11. Nanobodies against surface biomarkers enable the analysis of tumor genetic heterogeneity in uveal melanoma Patient Derived XenograftsRonan Crepin, David Gentien, Angeline Duche, Audrey Rapinat, Cecile Reyes, Fariba Nemati, Gerald Massonnet, Didier Deacaudin, Selma Djander, Sandrine Moutel, Klervi Even Desrumeaux, Nathalie Cassoux, Sophie Piperno-Neumann, Sebastian Amigorena, Franck Perez, Sergio Roman-Roman, Ario de Marco, 2017, original scientific article Abstract: Monoclonal antibodies specific for biomarkers expressed on the surface of uveal melanoma (UM) cells would simplify the immune-capture and genomic characterization of heterogeneous tumor cells originated from patient derived xenografts (PDXs). Antibodies against four independent tumor antigens were isolated by panning a nanobody synthetic library. Such antibodies enabled flow-cytometry-based sorting of distinct cell sub-populations from UM PDXs and to analyze their genomic features. The complexity and specificity of the biochemical and genomic biomarker combinations mirrored the UM tumor polyclonality. The data showed that MUC18 is highly and universally displayed at the surface of UM cells with different genetic background and consequently represents a reliable pan-biomarker for their identification and purification. In contrast, the other three biomarkers were detected in very variable combinations in UM PDX cells. The availability of the identified nanobodies will be instrumental in developing clone-specific drug evaluation and rational clinical strategies based on accurate genomic profiling
Found in: osebi
Keywords: nanobodies, uveal melanoma, patient derived xenografts, MUC18, membrane surface biomarkers, panning, tumor polyclonality
Published: 19.04.2017; Views: 4262; Downloads: 0
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15. Quantification of Circulating Cancer Biomarkers via Sensitive Topographic Measurements on Single Binder NanoarraysElena Ambrosetti, Pamela Paoletti, Alessandro Bosco, Pietro Parisse, Denis Scaini, Elda Tagliabue, Ario de Marco, Loredana Casalis, 2017, original scientific article Found in: osebi
Keywords: Biosensors, immuno-capture, nanobodies, HER2, cancer diagostics
Published: 22.06.2017; Views: 2778; Downloads: 145
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20. Urinary Extracellular Vesicle Biomarkers in Urological Cancers: From Discovery Towards Clinical ImplementationBert Dhondt, Jan Van Deun, Silke Vermaerke, Ario de Marco, Nicolaas Lumen, Olivier De Wever, An Hendrix, 2018, review article Found in: osebi
Keywords: Extracellular vesicles, cancer biomarkers, diagnostics
Published: 04.05.2018; Views: 2573; Downloads: 0
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