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2. Nanobodies: towards rational design of immune-reagentsArio De Marco, 2017, published scientific conference contribution abstract (invited lecture) Abstract: Antibodies are irreplaceable reagents in both research and clinical practice. Despite their relevance, the structural complexity of conventional mono- and polyclonal antibodies (IgG) has always been a limit for their engineering towards reagents optimized for specific applications, such as in vivo diagnostics and therapy. Furthermore, their isolation is time consuming, their production expensive, and their functionalization results often in heterogeneous macromolecule populations. These drawbacks promoted the search for both innovative antibody isolation strategies and alternative scaffolds. In vitro panning of pre-immune collections of recombinant antibody fragments allows for the simple and fast recovery of binders. Since they did not undergo somatic maturation, their affinity for targets can be insufficient but on the other hand they can be rapidly mutated by standard molecular biology techniques to generate second-generation antibodies among which to identify clones with improved characteristics. Both stochastic and rational methods have been proposed for the optimization process. Random mutagenesis followed by panning at stringent conditions has been successful used to select binders with improved physical characteristics. Rational methods try to identify in silico key residues involved in the regulation of specific antibody features, such as stability or binding affinity. The accuracy of these methods usually depends on the calculation resources. In this perspective, smaller molecules can be analyzed “better” than larger because of their restricted number of residues. Nanobodies small dimensions have been long appreciated since enable better tissue penetration, shorter clearance time, higher yields. Now it becomes evident that this characteristic makes them also optimal objects for modeling.
Found in: ključnih besedah
Summary of found: ...recombinant antibody modeling, nanobody engineering, molecular dynamics and docking...
Keywords: recombinant antibody modeling, nanobody engineering, molecular dynamics and docking
Published: 21.03.2018; Views: 3027; Downloads: 0
 Fulltext (638,20 KB) |
3. Identification of New DR5 Agonistic Nanobodies and Generation of Multivalent Nanobody Constructs for Cancer TreatmentGolnaz Sadeghnezhad, Ema Romao, Robert Alvin Bernedo-Navarro, Sam Massa, Khosro Khajeh, Serge Muyldermans, Sadegh Hassania, 2019, original scientific article Found in: ključnih besedah
Keywords: death receptor 5, nanobody, TRAIL, cancer therapy, apoptosis
Published: 01.10.2019; Views: 1762; Downloads: 0
Fulltext (2,89 MB) |
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5. Effect of Humanizing Mutations on the Stability of the Llama Single-Domain Variable RegionMiguel Soler, Barbara Medagli, Jiewen Wang, Sandra Oloketuyi, Gregor Bajc, He Huang, Sara Fortuna, Ario de Marco, 2021, original scientific article Found in: ključnih besedah
Summary of found: ... nanobody framework, modeling, nanobody humanization, CDR grafting...
Keywords: nanobody framework, modeling, nanobody humanization, CDR grafting
Published: 27.01.2021; Views: 1080; Downloads: 33
Fulltext (2,00 MB) |
6. Research progress and applications of multivalent, multispecific and modified nanobodies for disease treatmentArio De Marco, He Huang, Xiaocang Cao, Haibin Yuan, Guangbo Kang, Jiewen Wang, 2022, original scientific article Found in: ključnih besedah
Summary of found: ... nanobody multimers, immunomodulation, intrabodies, imaging, nanobody functionalization...
Keywords: nanobody multimers, immunomodulation, intrabodies, imaging, nanobody functionalization
Published: 18.01.2022; Views: 372; Downloads: 6
 Fulltext (0,00 KB)
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