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Title:Bilirubin is an Endogenous Antioxidant in Human Vascular Endothelial Cells
Authors:Žiberna, Lovro (Author)
Martelanc, Mitja (Author)
Franko, Mladen (Author)
Passamonti, Sabina (Author)
Files:.pdf srep29240.pdf (585,86 KB)
 
.zip srep29240-s1.pdf.zip (314,62 KB)
 
Language:English
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:Bilirubin is a standard serum biomarker of liver function. Inexplicably, it is inversely correlated with cardiovascular disease risk. Given the role of endothelial dysfunction in originating cardiovascular diseases, direct analysis of bilirubin in the vascular endothelium would shed light on these relationships. Hence, we used high-performance liquid chromatography coupled with thermal lens spectrometric detection and diode array detection for the determination of endogenous cellular IXα-bilirubin. To confirm the isomer IXα-bilirubin, we used ultra-performance liquid chromatography coupled with a high-resolution mass spectrometer using an electrospray ionization source, as well as tandem mass spectrometric detection. We measured bilirubin in both arterial and venous rat endothelium (0.9–1.5 pmol mg−1 protein). In the human endothelial Ea.hy926 cell line, we demonstrated that intracellular bilirubin (3–5 pmol mg−1 protein) could be modulated by either extracellular bilirubin uptake, or by up-regulation of heme oxygenase-1, a cellular enzyme related to endogenous bilirubin synthesis. Moreover, we determined intracellular antioxidant activity by bilirubin, with EC50 = 11.4 ± 0.2 nM, in the range of reported values of free serum bilirubin (8.5–13.1 nM). Biliverdin showed similar antioxidant properties as bilirubin. We infer from these observations that intra-endothelial bilirubin oscillates, and may thus be a dynamic factor of the endothelial function.
Keywords:Antioxidants, bilirubin, endothelial cells
Year of publishing:2016
Number of pages:6
Numbering:29240, 6
COBISS_ID:4431099 Link is opened in a new window
URN:URN:SI:UNG:REP:LMXAR9KU
DOI:10.1038/srep29240 Link is opened in a new window
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Downloads:244
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Record is a part of a journal

Title:Scientific Reports
Shortened title:Sci. Rep.
Publisher:Nature Publishing Group: Open Access Journals - Option C
ISSN:2045-2322
Year of publishing:2016

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