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Title:Biotinylated Vanadium and Chromium Sulfide Nanoparticles as Probes for Colocalization of Membrane Proteins
Authors:Loukanov, Alexandre (Author)
Emin, Saim (Author)
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Language:Slovenian
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:ABSTRACT We report the microemulsion synthesis of vanadium and chromium sulfide nanoparticles (NPs) and their biological application as nanoprobes for colocalization of membrane proteins. Spherical V2S3 and Cr2S3 NPs were prepared in reverse microemulsion droplets, as nanoreactors, obtained by the surfactant sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in nonpolar organic phase (heptane). Electron microscopic data indicated that the size distribution of the nanoparticles was uniform with an average diameter between 3 4 5 nm. The prepared hydrophobic nanocrystals were transferred in aqueous phase by surface cap exchange of AOT with biotindihydrolipoic ligands. This substitution allows the nanoparticles solubility in aqueous solutions and confer their bioactivity. In addition, we report the conjugation procedure between a-Lipoic acid (LA) and biotin (abbreviated as biotin-LA). The biotin-LA structure was characterized by 1D and 2D NMR spectroscopy. The biotinylated vanadium and chromium sulfide nanoparticles were tested as probes for colocalization of glutamate receptors on sodium-dodecyl-sulfate-digested replica prepared from rat hippocampus. The method suggests their high labeling efficiency for study of membrane biological macromolecules.
Keywords:biotin-dihydrolipoic conjugate, vanadium and chromium sulfide nanoparticles, membrane proteins
Year of publishing:2016
Number of pages:79
Numbering:DOI: 10.1002/jemt.22701, 2016
COBISS_ID:4655099 Link is opened in a new window
URN:URN:SI:UNG:REP:U8CKUTQW
DOI:10.1002/jemt.22701 Link is opened in a new window
License:CC BY 4.0
This work is available under this license: Creative Commons Attribution 4.0 International
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Title:MICROSCOPY RESEARCH AND TECHNIQUE
Year of publishing:2016

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