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Title:Whole-cell biopanning with a synthetic phage display library of nanobodies enabled the recovery of follicle-stimulating hormone receptor inhibitors
Authors:Crepin, Ronan (Author)
Veggiani, Gianluca (Author)
Djender, Selma (Author)
Beugnet, Anne (Author)
Planeix, Francois (Author)
Pichon, Christophe (Author)
Moutel, Sandrine (Author)
Amigorena, Sebastian (Author)
Pérez, Franck (Author)
Ghinea, Nicolae (Author)
De Marco, Ario (Author)
et al.
Files:This document has no files. This document may have a phisical copy in the library of the organization, check the status via COBISS. Link is opened in a new window
Language:English
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:Antibodies are essential reagents that are increasingly used in diagnostics and therapy. Their specificity and capacity to recognize their native antigen are critical characteristics for their in vivo application. Follicle-stimulating hormone receptor is a GPCR protein regulating ovarian follicular maturation and spermatogenesis. Recently, its potentiality as a cancer biomarker has been demonstrated but no antibody suitable for in vivo tumor targeting and treatment has been characterized so far. In this paper we describe the first successful attempt to recover recombinant antibodies against the FSHR and that: i) are directly panned from a pre-immune library using whole cells expressing the target receptor at their surface; ii) show inhibitory activity towards the FSH-induced cAMP accumulation; iii) do not share the same epitope with the natural binder FSH; iv) can be produced inexpensively as mono- or bivalent functional molecules in the bacterial cytoplasm. We expect that the proposed biopanning strategy will be profitable to identify useful functional antibodies for further members of the GPCR class.
Keywords:nanobodies, GPCR proteins, FSHR, panning, pre-immune antibody library
Year of publishing:2017
Number of pages:1567-1572
Numbering:493
COBISS_ID:4933627 Link is opened in a new window
URN:URN:SI:UNG:REP:4WYPVXVK
License:CC BY-NC-ND 4.0
This work is available under this license: Creative Commons Attribution Non-Commercial No Derivatives 4.0 International
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Record is a part of a journal

Title:Biochemical Biophysical Research Communications
ISSN:0006-291X

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