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Title:Headspace volatile organic compounds from bacteria implicated in ventilator-associated pneumonia analysed by TD-GC/MS
Authors:Lawal, Oluwasola (Author)
Muhamadali, Howbeer (Author)
Ahmed, Waqar M (Author)
White, Iain R (Author)
Nijsen, Tamara M E (Author)
Goodacre, Roy (Author)
Fowler, Stephen J (Author)
et al.
Files:This document has no files. This document may have a phisical copy in the library of the organization, check the status via COBISS. Link is opened in a new window
Language:English
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:Ventilator-associated pneumonia (VAP) is a healthcare-acquired infection arising from the invasion of the lower respiratory tract by opportunistic pathogens in ventilated patients. The current method of diagnosis requires the culture of an airway sample such as bronchoalveolar lavage, which is invasive to obtain and may take up to seven days to identify a causal pathogen, or indeed rule out infection. While awaiting results, patients are administered empirical antibiotics; risks of this approach include lack of effect on the causal pathogen, contribution to the development of antibiotic resistance and downstream effects such as increased length of intensive care stay, cost, morbidity and mortality. Specific biomarkers which could identify causal pathogens in a timely manner are needed as they would allow judicious use of the most appropriate antimicrobial therapy. Volatile organic compound (VOC) analysis in exhaled breath is proposed as an alternative due to its non-invasive nature and its potential to provide rapid diagnosis at the patient's bedside. VOCs in exhaled breath originate from exogenous, endogenous, as well as microbial sources. To identify potential markers, VAP-associated pathogens Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus were cultured in both artificial sputum medium and nutrient broth, and their headspaces were sampled and analysed for VOCs. Previously reported volatile markers were identified in this study, including indole and 1-undecene, alongside compounds that are novel to this investigation, cyclopentanone and 1-hexanol. We further investigated media components (substrates) to identify those that are essential for indole and cyclopentanone production, with potential implications for understanding microbial metabolism in the lung.
Keywords:bacteria, exhaled breath, infection, ventilator-associated pneumonia, volatile organic compounds
Year of publishing:2018
Number of pages:11
Numbering:12, 2
COBISS_ID:5418747 Link is opened in a new window
URN:URN:SI:UNG:REP:TTXG02BJ
DOI:10.1088/1752-7163/aa8efc Link is opened in a new window
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Record is a part of a journal

Title:Journal of Breath Research
Shortened title:JBR
Publisher:Institute of Physics Publishing
ISSN:1752-7155

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