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Title:Determination of biogenic amines formation by autochthonous lactic acid bacteria from ‘Refošk’ grapes using different analytical methods
Authors:Topić, Jelena (Author)
Butinar, Lorena (Author)
Bergant Marušič, Martina (Author)
Korte, Dorota (Author)
Mozetič Vodopivec, Branka (Author)
Files:URL https://doi.org/10.1016/j.lwt.2021.112908
 
Language:English
Work type:Unknown ()
Tipology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:Different analytical methods were tested and optimized for the determination of four biogenic amines (BA) histamine, putrescine, cadaverine and tyramine produced by grape-associated lactic acid bacteria (LAB). Autochthonous LAB were isolated from ‘Refošk’ grapes belonging to Slovenian-Italian Karst region as they represent a potential pool of beneficial LAB starter bacteria for improving the typicality and quality of wine. Six isolated strains were screened by multiplex PCR, of which four strains were positive for BA-forming genes (MKBT-49, MKBT-282, MKBT-568, MKBT-570). The production of BA was evaluated by thin-layer chromatography (TLC), high-performance liquid chromatography coupled to diode array detection (HPLC-DAD) and enzymatic method. The HPLC-DAD results showed that strain MKBT-49 (tyrdc+) had high tyramine production (386.6 ± 0.14 mg/L), which was confirmed by TLC. The ability to produce putrescine was confirmed in strain MKBT-282 by PCR, HPLC-DAD (16.4 ± 1.72 mg/L), and TLC. Histamine-producing ability was detected in strain MKBT-570, with a concentration below the limit of detection of the HPLC-DAD (<0.2 mg/L), while the other two methods were not sensitive enough for confirmation. This study shows that the production of BA can detected in native LAB and that relatively simple method such as TLC can be used effectively for the initial screening.
Keywords:grapes, wine-like matrices, biogenic amines, autochthonous lactic acid bacteria, analytical methods
Year of publishing:2022
Number of pages:str. 1-12
Numbering:Vol. 156, 156
COBISS_ID:91673091 Link is opened in a new window
UDC:614
ISSN on article:1096-1127
URN:URN:SI:UNG:REP:VSZF6K66
DOI:10.1016/j.lwt.2021.112908 Link is opened in a new window
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Record is a part of a journal

Title:LWT - Food science and technology
Shortened title:Food sci. technol.
Publisher:Swiss Society of Food Science and Technology by Academic Press
ISSN:1096-1127
COBISS.SI-ID:49102083 New window

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