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2. Differential scanning calorimetry of proteins and the two-state model : comparison of two formulasKnarik Yeritsyan, Artem Badasyan, 2024, original scientific article Abstract: Differential Scanning Calorimetry (DSC) is a regular and powerful tool to measure the specific heat profile of various materials. In order to connect the measured profile to the properties of a particular protein, a model is required to fit. We discuss here the application of an exact two-state formula with its approximation and process the DSC experimental data on protein folding in water. The approximate formula relies on the smallness of the transition interval, which is different for each protein. With an example of the set of 33 different proteins, we show the practical validity of the approximation and the equivalence of exact and approximate two-state formulas for processing DSC data. Keywords: protein folding, differential scanning calorimetry, heat capacity, two-state model, Hawley model Published in RUNG: 21.05.2024; Views: 355; Downloads: 3
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3. Modelling water for calorimetry of proteinsKnarik Yeritsyan, Artem Badasyan, 2023, published scientific conference contribution abstract Abstract: Differential Scanning Calorimetry (DSC) is a powerful technique used to study the thermal stability and unfolding of proteins. DSC provides the excess heat capacity profile and is used to study the thermodynamics of a given protein. By fitting DSC data to the model, researchers can obtain valuable information about the thermodynamics of protein folding and unfolding, which can help them better understand protein structure, stability, and function.
Based on Hamiltonian representation of ZB model and using the solvent effects we derived an expression for heat capacity in proteins and successfuly fit it to experimental data. As we show, our model provides a better fit to experimental data, as compared to the 2-state model. The model we propose takes into account also water effects and we show that it fits better to experimental data giving inter- and intra-molecular H-bonding energies instead of reporting only one total enthalpy. Keywords: Zimm-Bragg model, water model, helix-coil transition, protein folding, differential scanning calorimetry Published in RUNG: 18.10.2023; Views: 948; Downloads: 0 This document has many files! More... |
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