1. Measurement campaign of Icelandic volcanic ash during its arrival to Slovenia in April 2010Fei Gao, Samo Stanič, Klemen Bergant, Tanja Bolte, Franco Coren, Tingyao He, Andrej Hrabar, Ana Mladenovič, Janja Turšič, Darko Veberič, 2011, published scientific conference contribution abstract Found in: osebi Keywords: vulkanski pepel, atmosfera, daljinsko zaznavanje Published: 15.10.2013; Views: 5678; Downloads: 136
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4. MODIFIED U1 RNAs AS SPLICING CORRECTORS IN HUMAN GENETIC DISORDERSKatarzyna Rajkowska, 2018, doctoral dissertation Abstract: The experimental work of this thesis was performed at the International Centre
for Genetic Engineering and Biotechnology (ICGEB) in the Human Molecular
Genetics Group, under the scientific direction of Prof. Franco Pagani. The project
was developed during the academic years 2014-2017.
Modified U1 RNAs, also named Exon Specific U1s (ExSpeU1s) represent a novel
class of small RNA-based molecules that correct exons splicing defects. To evaluate
their therapeutic potential focused on Familial Dysautonomia (FD), a rare autosomal
recessive disorder characterized by progressive degeneration of the sensory and
autonomic nervous system. More than 99% of patients are homozygous for the T to
C transition in position 6 of the IKBKAP intron 20 (c.2204+6T>C). This substitution
modifies the exon 20 5’ splice site (5’ss) inducing exon skipping in a tissue-specific
manner and reducing the total amount of IKAP protein. The molecular mechanisms
underlying the IKBKAP mis-splicing are not completely clear and there
are no effective treatments.
In this thesis, I investigated the therapeutic potential of ExSpeU1s and the role
of cis- and trans-acting factors that regulates IKBKAP splicing. Using a splicing
functional assay, I identified ExSpeU1s that bind to intron 20 sequences and rescue
the exon 20 skipping defect. Interestingly, their rescue activity was modulated
by several splicing factors and requires a critical exonic splicing enhancer element.
Transfection experiment showed the involvement of both enhancing (TIA1, PTBP1
and PTB4) and inhibitory (SRSF3, hnRNPA1, FOX and FUS) splicing factors
in IKBKAP splicing. To better evaluate the ExSpeU1s therapeutic efficacy,
I transduced FD patient’s fibroblasts with a lentiviral vector expressing the most
active ExSpeU1. This resulted in a complete rescue of the exon skipping defect and
improvement in IKAP protein expression. Most importantly, intraperitoneal delivery
of ExSpeU1s by AAV9 into a transgenic mouse model, that recapitulates
the tissue-specific mis-splicing seen in FD patients, corrected the aberrant splicing
patterns in several tissues increasing the amount of the corresponding IKAP protein.
All together, these results identify novel regulatory splicing factors involved in the
IKBKAP exon 20 regulation and provide the proof of principle that ExSpeU1s
delivered in vivo by AAV vectors represent a novel therapeutic strategy for FD. Found in: osebi Summary of found: ...Group, under the scientific direction of Prof. Franco Pagani. The project
was developed during the academic... Keywords: Familial Dysautonomia, IKBKAP, IKAP, splicing, splicing defects, ExSpeU1, U1 snRNA, mouse model, AAV Published: 26.03.2018; Views: 3764; Downloads: 122
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5. Lotta guidata in viticolturaGiorgio Malossini, Paolo Sivilotti, M. Stocco, Giulio Franco, Carlo Frausin, 2012, other monographs and other completed works Found in: osebi Keywords: vinska trta, grozdje, škodljivci, plesni Published: 15.10.2013; Views: 6755; Downloads: 29
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6. XAS and micro-XRF analysis of mono and bi-metallic exopolysaccharide (FePd-EPS) bio-generated by K. oxytocaFranco Baldi, Katarina Vogel-Mikuš, Michele Gallo, Oreste Piccolo, Stefano Paganelli, Iztok Arčon, 2016, published scientific conference contribution abstract Abstract: Bacteria Klebsiella oxytoca (DSM 29614) secret a specific exopolysaccharide (EPS) in the presence of Fe(III)-citrate, as sole carbon and energy source, and produces after 7 days an iron gel precipitate (Fe-EPS) [1]. In the presence of palladium or other metal species, the culture of K. oxytoca can produce other mono or bimetal species (Pd-EPS, FePd-EPS) [2]. These bio-materials may be used as green biogenerated catalysts or for other biotechnological purposes. In this work we present the analysis of the morphology and the chemical state of the metals in mono and bi-metallic (Fe-EPS, Pd-EPS, FePd-EPS) complexes, by a combination of micro X-ray fluorescence, X-ray absorption spectroscopy methods (XANES and EXAFS), and transmission electron microscopy. The results show that iron in monometallic Fe-EPS and bimetalic FePd-EPS is in the mineralized form of iron oxides/hydroxides, predominantly in the form of Fe3+, with a small amount of Fe2+ in the structure, as a mixture of different nano-crystalline iron oxides and hydroxides. Palladium on the other hand is found as Pd(0) in the form of metallic nanoparticles with fcc structure in both, bi-metallic (FePd-EPS) and mono-metallic (Pd-EPS) species. In bi-metallic species Pd and Fe nanoparticles agglomerate in larger clusters, but they remain spatially separated. Access to the SR facilities at ESRF (beamline ID21, project LS-2225), DESY (beamline C) Hamburg (project I-20110511 EC) and ELETTRA (beamline XAFS, project 20115112) is acknowledged. Found in: osebi Keywords: Klebsiella oxytoca, Fe, Pd, XANES, EXAFS, exopolysaccharide, FePd-EPS Published: 28.06.2016; Views: 4905; Downloads: 0
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7. Alternative Recovery and Valorization of Metals from Exhausted Catalytic Converters in a New Smart Polymetallic CatalystOreste Piccolo, Laura Sperni, Michele Gallo, Katarina Vogel-Mikuš, Iztok Arčon, Franco Baldi, Sebastiano Tieuli, Stefano Paganelli, 2019, original scientific article Abstract: A new metals-polymeric composite, Metx-EPS (I), was prepared
to be used as catalyst in water or in two-phase aqueous
conditions. The metals source was an exhausted catalytic
converter that was grinded and treated with an acidic solution at room temperature. After filtration, the solution was concentrated, neutralized and added to a broth of Klebsiella oxytoca DSM 29614 to produce (I) where metals are embedded in a peculiar polysaccharide structure (EPS). The composite was easily recovered from the fermentation broth and purified. The process protocol was verified many times and was shown to be reproducible satisfactorily. The % recovery of metals, originally present in the converter, was good as determined by atomic
absorption. The morphology and the chemical state of main
metals in (I) were investigated by X-ray absorption spectroscopy
methods (XANES and EXAFS). No metallic alloy seems to
be evident. The catalytic activity and a possible synergic effect due to the presence of the different metals was valuated in the hydrogenation of some substrates, valuable precursors for theproduction of fine chemicals. Found in: osebi Keywords: Biogenerated polymetallic exopolysaccharide, biphasic catalysis, hydrogenation, metals-polymeric
composite, new catalyst from metallic wastes, EXAFS, XANES Published: 06.05.2019; Views: 2494; Downloads: 0
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8. A Biogenerated Polymetallic Catalyst from Society's WastesOreste Piccolo, Stefano Paganelli, Petro Zanatta, Sebastiano Tieuli, Laura Sperni, Franco Baldi, Iztok Arčon, Michele Gallo, Katarina Vogel-Mikuš, 2019, original scientific article Abstract: Aims:Preparation of the new metals - polymeric composite, Met x-EPS (I), to be used as a green catalyst in water or in two-phase aqueous conditions.
Study Design:
Recovery and valorization of polymetallic
wastes to obtain directly new catalysts
using a microorganism to explore their application in removal of difficult and dangerous chemical
pollutants present in aqueous environment Found in: osebi Keywords: Metals
-
polymeric composite, biogenerated polymetallic exopolysaccharide, new catalyst
from metallic wastes, hydrodechlorination
of PCBs in water Published: 06.05.2019; Views: 2703; Downloads: 0
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9. The first GCT camera for the Cherenkov Telescope ArrayMarko Zavrtanik, Danilo Zavrtanik, Gabrijela Zaharijas, Lili Yang, Serguei Vorobiov, Samo Stanič, Gašper Kukec Mezek, Andrej Filipčič, A. De Franco, 2015, published scientific conference contribution Found in: osebi Keywords: Cherenkov Telescope Array (CTA), small-size telescope (SST) design for CTA, dual-mirror (2M) Schwarzschild-Couder design, Gamma Cherenkov Telescope (GCT): SST-2M telescope design for CTA, imaging camera Published: 21.11.2016; Views: 3645; Downloads: 110
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10. An extracellular polymeric substance quickly chelates mercury(II) with N-heterocyclic groupsClaudia Faleri, Dario Battistel, Salvatore Daniale, Michele Gallo, Franco Baldi, Alojz Kodre, Iztok Arčon, 2017, original scientific article Abstract: A strain of Klebsiella oxytoca DSM 29614 is grown on sodium citrate in the presence of 50 mg l−1 of Hg as Hg(NO3)2. During growth, the strain produces an extracellular polymeric substance (EPS), constituted by a mixture of proteins and a specific exopolysaccharide. The protein components, derived from the outer membrane of cells, are co-extracted with the extracellular exopolysaccharide using ethanol. The extracted EPS contains 7.5% of Hg (total amount). This indicates that EPS is an excellent material for the biosorption of Hg2+, through chemical complexation with the EPS components. The binding capacity of these species towards Hg2+ is studied by cyclic voltammetry, and Hg L3-edge XANES and EXAFS spectroscopy. The results found indicate that Hg2+ is mainly bound to the nitrogen of the imidazole ring or other N-heterocycle compounds. The hydroxyl moities of sugars and/or the carboxyl groups of two glucuronic acids in the polysaccharide can also play an important role in sequestring Hg2+ ions. However, N-heterocyclic groups of proteins bind Hg2+ faster than hydroxyl and carboxyl groups of the polysaccharide. Found in: osebi Keywords: Klebsiella oxytoca, Biosorption, Proteins, Polysaccharide, Cyclic voltammetry, EXAFS analysis Published: 03.03.2017; Views: 3905; Downloads: 0
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