Repository of University of Nova Gorica

Search the repository
A+ | A- | Help | SLO | ENG

Query: search in
search in
search in
search in
* old and bologna study programme

Options:
  Reset


121 - 130 / 142
First pagePrevious page6789101112131415Next pageLast page
121.
122.
Nanobodies as biotechnological tools
Ario De Marco, invited lecture at foreign university

Keywords: nanobodies, pre-immune phage library, recombinant expression, biomarkers, tumors
Published in RUNG: 17.05.2017; Views: 4595; Downloads: 0
This document has many files! More...

123.
Biotechnological applications of nanobody pre-immune libraries
Ario De Marco, invited lecture at foreign university

Keywords: nanobodies, pre-immune libraries, in vitro selection, biomarkers, recombinant proteins
Published in RUNG: 16.05.2017; Views: 4846; Downloads: 0
This document has many files! More...

124.
Nanobody technology: principles & applications
Ario De Marco, invited lecture at foreign university

Abstract: Antibodies possess unattainable capacity to bind selectively and at high affinity their cognates. For this reason they have been largely used in applications which rely on specific molecular recognition. Biosensors, nanoparticles, and even cells can be functionalized with antibodies for improving sensitivity and target specificity. However, conventional antibodies (IgGs) are large molecules (150 kDa) that are difficult to engineer. In the last years, antibody fragments have become more and more popular as an effective alternative and specifically nanobodies raised enthusiasm because of their minimal mass (14 kDa), high stability, relative similarity to human sequences, and simplified mutagenesis. Pre-immune nanobody libraries have the further advantage of enabling blind selection for antigens that can be used to discriminate between subpopulations of cells and vesicles. The panning can be performed directly on intact cells and the resulting binders are specific for the native antigen conformation
Keywords: Nanobodies, in vitro panning, cancer biomarkers, recombinant antibody engineering
Published in RUNG: 03.05.2017; Views: 5962; Downloads: 0
This document has many files! More...

125.
Nanobodies against surface biomarkers enable the analysis of tumor genetic heterogeneity in uveal melanoma Patient Derived Xenografts
Ronan Crepin, David Gentien, Angeline Duche, Audrey Rapinat, Cecile Reyes, Fariba Nemati, Gerald Massonnet, Didier Deacaudin, Selma Djander, Sandrine Moutel, Klervi Even Desrumeaux, Nathalie Cassoux, Sophie Piperno-Neumann, Sebastian Amigorena, Franck Perez, Sergio Roman-Roman, Ario De Marco, 2017, original scientific article

Abstract: Monoclonal antibodies specific for biomarkers expressed on the surface of uveal melanoma (UM) cells would simplify the immune-capture and genomic characterization of heterogeneous tumor cells originated from patient derived xenografts (PDXs). Antibodies against four independent tumor antigens were isolated by panning a nanobody synthetic library. Such antibodies enabled flow-cytometry-based sorting of distinct cell sub-populations from UM PDXs and to analyze their genomic features. The complexity and specificity of the biochemical and genomic biomarker combinations mirrored the UM tumor polyclonality. The data showed that MUC18 is highly and universally displayed at the surface of UM cells with different genetic background and consequently represents a reliable pan-biomarker for their identification and purification. In contrast, the other three biomarkers were detected in very variable combinations in UM PDX cells. The availability of the identified nanobodies will be instrumental in developing clone-specific drug evaluation and rational clinical strategies based on accurate genomic profiling
Keywords: nanobodies, uveal melanoma, patient derived xenografts, MUC18, membrane surface biomarkers, panning, tumor polyclonality
Published in RUNG: 19.04.2017; Views: 6566; Downloads: 0
This document has many files! More...

126.
Nanobody technology: principles & applications
Ario De Marco, unpublished invited conference lecture

Keywords: nanobodies, nanotechnologies, biomarker identification
Published in RUNG: 29.03.2017; Views: 5454; Downloads: 0
This document has many files! More...

127.
Is there an approach for optimizing the specific binding of antibodies to target cells?
Ario De Marco, invited lecture at foreign university

Keywords: Nanobodies, avidity, modeling, off-target accumulation
Published in RUNG: 31.01.2017; Views: 5258; Downloads: 0
This document has many files! More...

128.
Assessment of the actual antibody-antigen binding conditions at the surface of biological membranes
Ario De Marco, 2016, published scientific conference contribution abstract (invited lecture)

Keywords: Exosomes, avidity effect, AFM
Published in RUNG: 30.11.2016; Views: 5713; Downloads: 0
This document has many files! More...

129.
Molecular dynamics simulations and docking enable to explore the biophysical factors controlling the yields of engineered nanobodies
Miguel Soler, Ario De Marco, Sara Fortuna, 2016, original scientific article

Abstract: Nanobodies (VHHs) have proved to be valuable substitutes of conventional antibodies for molecular recognition. Their small size represents a precious advantage for rational mutagenesis based on modelling. Here we address the problem of predicting how Camelidae nanobody sequences can tolerate mutations by developing a simulation protocol based on all-atom molecular dynamics and wholemolecule docking. The method was tested on two sets of nanobodies characterized experimentally for their biophysical features. One set contained point mutations introduced to humanize a wild type sequence, in the second the CDRs were swapped between single-domain frameworks with Camelidae and human hallmarks. The method resulted in accurate scoring approaches to predict experimental yields and enabled to identify the structural modifications induced by mutations. This work is a promising tool for the in silico development of single-domain antibodies and opens the opportunity to customize single functional domains of larger macromolecules
Keywords: nanobodies, molecular dynamics, modeling, antibody solubility
Published in RUNG: 11.10.2016; Views: 5652; Downloads: 249
.pdf Full text (1,95 MB)

130.
Nanobodies for research & clinical applications
Ario De Marco, invited lecture at foreign university

Abstract: Nanobody technology evolved rapidly in the last years and now is available for applications in basic sciences as well as in diagnostics and therapy
Keywords: nanobodies, antibody functionalization, phage display panning, recombinant antibody production
Published in RUNG: 11.10.2016; Views: 5961; Downloads: 0
This document has many files! More...

Search done in 0.04 sec.
Back to top