1.
Laser ablation-single-particle-inductively coupled plasma mass spectrometry as a multimodality bioimaging tool in nano-based omicsDino Metarapi,
Johannes Teun van Elteren,
Martin Šala,
Katarina Vogel-Mikuš,
Iztok Arčon,
Vid Simon Šelih,
Mitja Kolar,
Samo B. Hočevar, 2021, original scientific article
Abstract: Advanced data processing and visualization techniques were applied to laser ablation-single particleinductively
coupled plasma mass spectrometry (LA-sp-ICPMS) data obtained from imaging of roots of
sunflower (Helianthus annuus L.) which were exposed to ionic silver (Ag+). Highly multiplexed images with
tissue level resolution were generated, showing the uptake and transformation of Ag+ to silver
nanoparticles (AgNPs) in whole root cross-sections, and revealing that the size of biosynthesized AgNPs is
predisposed by the reducing power of particular root compartments. Various Ag+ and AgNP visualization
strategies are shown with special emphasis on spatially resolved information associated with the number
and spherical-equivalent size of individual AgNPs in user-selected root cross-sectional regions, including
statistical analysis of the AgNP distribution. Normally, this multimodality information can only be obtained
by combining the results of techniques from several complementary platforms.
Keywords: Laser ablation, Ag XANES, bioimaging
Published in RUNG: 03.06.2021; Views: 3130; Downloads: 51
Link to full text
This document has many files! More...
