1. Laser ablation-single-particle-inductively coupled plasma mass spectrometry as a multimodality bioimaging tool in nano-based omicsDino Metarapi, Johannes Teun van Elteren, Martin Šala, Katarina Vogel-Mikuš, Iztok Arčon, Vid Simon Šelih, Mitja Kolar, Samo B. Hočevar, 2021, original scientific article Abstract: Advanced data processing and visualization techniques were applied to laser ablation-single particleinductively
coupled plasma mass spectrometry (LA-sp-ICPMS) data obtained from imaging of roots of
sunflower (Helianthus annuus L.) which were exposed to ionic silver (Ag+). Highly multiplexed images with
tissue level resolution were generated, showing the uptake and transformation of Ag+ to silver
nanoparticles (AgNPs) in whole root cross-sections, and revealing that the size of biosynthesized AgNPs is
predisposed by the reducing power of particular root compartments. Various Ag+ and AgNP visualization
strategies are shown with special emphasis on spatially resolved information associated with the number
and spherical-equivalent size of individual AgNPs in user-selected root cross-sectional regions, including
statistical analysis of the AgNP distribution. Normally, this multimodality information can only be obtained
by combining the results of techniques from several complementary platforms. Keywords: Laser ablation, Ag XANES, bioimaging Published in RUNG: 03.06.2021; Views: 3472; Downloads: 51
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2. Localization, ligand environment, bioavailability and toxicity of mercury in Boletus spp. and Scutiger pes-caprae mushroomsAnja Kavčič, Klemen Mikuš, Marta Debeljak, Johannes Teun van Elteren, Iztok Arčon, Alojz Kodre, Peter Kump, Andreas-Germanos Karydas, Alessandro Migliori, Mateusz Czyzycki, Katarina Vogel-Mikuš, 2019, original scientific article Abstract: This study provides information on mercury (Hg) localization, speciation and ligand environment in edible mushrooms: Boletus edulis, B. aereus and Scutiger pes-caprae collected at non-polluted and Hg polluted sites, by LA-ICP-MS, SR-μ-XRF and Hg L3-edge XANES and EXAFS. Mushrooms (especially young ones) collected at Hg polluted sites can contain more than 100 μg Hg g−1 of dry mass. Imaging of the element distribution shows that Hg accumulates mainly in the spore-forming part (hymenium) of the cap. Removal of hymenium before consumption can eliminate more than 50% of accumulated Hg.
Mercury is mainly coordinated to di-thiols (43–82%), followed by di-selenols (13–35%) and tetra-thiols (12–20%). Mercury bioavailability, as determined by feeding the mushrooms to Spanish slugs (known metal bioindicators owing to accumulation of metals in their digestive gland), ranged from 4% (S. pes-caprae) to 30% (B. aereus), and decreased with increasing selenium (Se) levels in the mushrooms. Elevated Hg levels in mushrooms fed to the slugs induced toxic effects, but these effects were counteracted with increasing Se concentrations in the mushrooms, pointing to a protective role of Se against Hg toxicity through HgSe complexation. Nevertheless, consumption of the studied mushroom species from Hg polluted sites should be avoided. Keywords: edible mushrooms, HgSe complex, imaging of elemental distribution, LA-ICP-MS, alpha-XRF, XAS Published in RUNG: 24.10.2019; Views: 5841; Downloads: 0 This document has many files! More... |
3. Recent advances in 2D imaging of element distribution in Plants by focused beam techniquesKatarina Vogel-Mikuš, Johannes Teun van Elteren, Marjana Regvar, Jitrin Chaiprapa, Boštjan Jenčič, Iztok Arčon, Alojz Kodre, Peter Kump, Anja Kavčič, Mitja Kelemen, Dino Metarapi, Marijan Nečemer, Primož Vavpetič, Primož Pelicon, Paula Pongrac, 2019, independent scientific component part or a chapter in a monograph Keywords: 2D Imiging, XRF, XANES, synchrotron light Published in RUNG: 05.09.2019; Views: 4367; Downloads: 0 This document has many files! More... |
4. Biotransformation of copper oxide nanoparticles by the pathogenic fungus Botrytis cinereaEva Kovačec, Marjana Regvar, Johannes Teun van Elteren, Iztok Arčon, Tamás Papp, Darko Makovec, Katarina Vogel-Mikuš, 2017, original scientific article Abstract: Two plant pathogenic fungi, Botrytis cinerea and Alternaria alternata, isolated from crop plants, were
exposed to Cu in ionic (Cu2þ), microparticulate (MP, CuO) or nanoparticulate (NP, Cu or CuO) form, in
solid and liquid culturing media in order to test fungal response and toxic effects of the mentioned
compounds for the potential use as fungicides. B. cinerea has shown pronounced growth and lower levels
of lipid peroxidation compared to A. alternata. Its higher resistance/tolerance is attributed mainly to
biotransformation of CuO and Cu NPs and CuO MPs into a blue compound at the fungal/culturing media
interface, recognized by Cu K-edge EXAFS analysis as Cu-oxalate complex. The pronounced activity of
catechol-type siderophores and organic acid secretion in B. cinerea induce leaching and mobilization of
Cu ions from the particles and their further complexation with extracellularly secreted oxalic acid. The
ability of pathogenic fungus to biotransform CuO MPs and NPs hampers their use as fungicides. However
the results show that B. cinerea has a potential to be used in degradation of Cu(O) nanoparticles in
environment, copper extraction and purification techniques. Keywords: copper, metal oxide nanoparticles, detoxification mechanisms, metal pollution, Cu-oxalate Published in RUNG: 23.08.2017; Views: 5506; Downloads: 0 This document has many files! More... |
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